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Product Details
Synonyms
IL7Ra, CD127
Source
Human IL-7 R alpha Protein, His Tag (IL7-H52H7) is expressed from human 293 cells (HEK293). It contains AA Glu 21 - Asp 239 (Accession # P16871-1).
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 27.3 kDa. The protein migrates as 38-48 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Human IL-7 R alpha Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
Bioactivity-ELISA
Immobilized Human IL-7, Fc Tag (Cat. No. IL7-H5253) at 5 μg/mL (100 μL/well) can bind Human IL-7 R alpha Protein, His Tag (Cat. No. IL7-H52H7) with a linear range of 0.002-0.313 μg/mL (QC tested).
Protocol
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Background
Recent Advances
Drug Development Progress- English Name：
Interleukin-7 receptor subunit alpha
- Category：
- Approved Drugs：
0 Details
- Drugs in Clinical Trials：
8 Details
- Highest Development Stage：
Phase 2 Clinical
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