Product Details
Synonyms
IL-23 alpha & IL-12 beta
Source
Human IL-23A&Alpaca IL-12B Heterodimer Protein, His Tag&Tag Free (ILB-HA52W3) is expressed from human 293 cells (HEK293). It contains AA Arg 20 - Pro 189 & Ile 23 - Ser 330 (Accession # Q9NPF7 & A0A6I9IGP5).
Predicted N-terminus: Arg 20 & Ile 23
Request for sequenceMolecular Characterization

Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 20.6 kDa & 34.8 kDa. The protein migrates as 22-23 kDa and 38-41 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
The protein is designed as a heterodimer.
Endotoxin
Less than 1.0 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE

Human IL-23A&Alpaca IL-12B Heterodimer Protein, His Tag&Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS

The purity of Human IL-23A&Alpaca IL-12B Heterodimer Protein, His Tag&Tag Free (Cat. No. ILB-HA52W3) is more than 90% and the molecular weight of this protein is around 50-65 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA

Immobilized Human IL-23 R, Fc Tag (Cat. No. ILR-H5254) at 5 μg/mL (100 μL/well) can bind Human IL-23A&Alpaca IL-12B Heterodimer Protein, His Tag&Tag Free (Cat. No. ILB-HA52W3) with a linear range of 2-156 ng/mL (QC tested).
Protocol
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Recent Advances
- English Name:
Interleukin-12 subunit beta
- Category:
- Approved Drugs:
7 Details
- Drugs in Clinical Trials:
2 Details
- Highest Development Stage:
Approved
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