Product Details
Synonyms
Streptavidin, SA
Source
Recombinant Streptavidin Protein (STN-N5113) is expressed from E. coli cells. It contains AA Ala 37 - Ser 163 (Accession # P22629-1).
Predicted N-terminus: Met
Request for sequenceMolecular Characterization

Other Tags and Version Biotin & Other Labeled Version
This protein carries no "tag".
The protein has a calculated MW of 13.4 kDa. The protein migrates as 11-12 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).
The protein is designed as a tetramer.
Endotoxin
Less than 0.1 EU per μg by the LAL method / rFC method.
Sterility
Negative
Purity
>90% as determined by SDS-PAGE.
>95% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in 0.02% NaCl, pH7.3.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE

Recombinant Streptavidin Protein on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS

The purity of Recombinant Streptavidin Protein (Cat. No. STN-N5113) is more than 95% and the molecular weight of this protein is around 45-65 kDa verified by SEC-MALS.
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Bioactivity-ELISA

Immobilized Recombinant Streptavidin Protein (Cat. No. STN-N5113) can bind Biotinylated Human PVRIG, Fc,Avitag (Cat. No. PVG-H82F4) at 1 μg/mL (100 μL/well) with a linear range of 0.2-6 ng/mL (QC tested).
Protocol
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