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Biotinylated Cynomolgus VEGF-C Protein, His,Avitag™ (MALS verified)

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VEC-C82E4-25ug
$350.00
VEC-C82E4-200ug
$1380.00
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Product Details

  • Synonyms

    VEGFC, Flt4-L, VRP

  • Source

    Biotinylated Cynomolgus VEGF-C Protein, His,Avitag (VEC-C82E4) is expressed from human 293 cells (HEK293). It contains AA Thr 104 - Arg 228 (Accession # A0A2K5WBI9).

    Predicted N-terminus: Thr 104

    Request for sequence
  • Molecular Characterization

    VEGF-C Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    The protein has a calculated MW of 17.6 kDa. The protein migrates as 25-30 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

    The protein is designed as a dimer.

  • Labeling

    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

  • Protein Ratio

    Passed as determined by the HABA assay / binding ELISA.

  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    VEGF-C SDS-PAGE

    Biotinylated Cynomolgus VEGF-C Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

  • SEC-MALS

    VEGF-C SEC-MALS

    The purity of Biotinylated Cynomolgus VEGF-C Protein, His,Avitag (Cat. No. VEC-C82E4) is more than 90% and the molecular weight of this protein is around 40-55 kDa verified by SEC-MALS.

    Report
  • Bioactivity-ELISA

     VEGF-C ELISA

    Immobilized Human VEGF R2, Fc Tag (Cat. No. VE2-H5255) at 5 μg/mL (100 μL/well) can bind Biotinylated Cynomolgus VEGF-C Protein, His,Avitag (Cat. No. VEC-C82E4) with a linear range of 0.2-5 ng/mL (QC tested).

    Protocol

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Background

Vascular endothelial growth factor C is also known as VEGFC, Flt4-L and VRP, it contains the C-terminal propeptide which has an unusual structure with tandemly repeated cysteine-rich motifs. Upon biosynthesis, VEGFC is secreted as a non-covalent momodimer in an anti-parellel fashion. VEGFC is a member of the platelet-derived growth factor/vascular endothelial growth factor (PDGF/VEGF) family, is active in angiogenesis, lymphangiogenesis and endothelial cell growth and survival, and can also affect the permeability of blood vessels. This secreted protein undergoes a complex proteolytic maturation, generating multiple processed forms that bind and activate VEGFR-3 receptors. Only the fully processed form can bind and activate VEGFR-2 receptors. The structure and function of this protein is similar to those of vascular endothelial growth factor D (VEGF-D). VEGFC may function in angiogenesis of the venous and lymphatic vascular systems during embryogenesis, and also in the maintenance of differentiated lymphatic endothelium in adults. Overexpression of VEGF-C causes lymphatics to enlarge possibly facilitates metastasis.

Recent Advances

 
Drug Development Progress
  • English Name:

    Vascular endothelial growth factor C

  • Category:

  • Approved Drugs:

    0 Details

  • Drugs in Clinical Trials:

    2 Details

  • Highest Development Stage:

    Phase 2 Clinical

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