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Product Details
Synonyms
Integrin alpha 4 beta 1, ITGA4&ITGB1
Source
Human ITGA4&ITGB1 Heterodimer Protein, His Tag&Tag Free (IT1-H52W1) is expressed from human 293 cells (HEK293). It contains AA Tyr 34 - Thr 977 (ITGA4) & Gln 21 - Asp 728 (ITGB1) (Accession # P13612-1 (ITGA4) & P05556-1 (ITGB1)).
Predicted N-terminus: Tyr 34 & Ser 592 (ITGA4) & Gln 21 (ITGB1)
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
Human ITGA4&ITGB1 Heterodimer Protein, His Tag&Tag Free, produced by co-expression of ITGA4 and ITGB1, has a calculated MW of 111.5 kDa (ITGA4) and 83.7 kDa (ITGB1). Subunit ITGA4 is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB1 contains no tag but a basic tail at the C-terminus. The ITGA4 subunit is composed of a heavy chain (Tyr 34 - Arg 591, calculated MW 61.1 kDa) and a light chain (Ser 592 - Thr 977, calculated MW 50.4 kDa). Consequently ITGA4 migrates as 60-75 kDa, and ITGB1 as 110-140 kDa when calibrated against Star Ribbon Pre-stained Protein Marker respectively under non-reducing (NR) condition due to cleavage and glycosylation.
Endotoxin
Less than 0.01 EU per μg by the LAL method / rFC method.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
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Performance Data
SDS-PAGE
Human ITGA4&ITGB1 Heterodimer Protein, His Tag&Tag Free on SDS-PAGE under non-reducing (NR) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
Bioactivity-ELISA
Immobilized Natalizumab at 1 μg/mL (100 μL/well) can bind Human ITGA4&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-H52W1) with a linear range of 1-16 ng/mL (QC tested).
Protocol
Bioactivity-SPR
Human VCAM-1, His Tag (Cat. No. VC1-H5224) immobilized on CM5 Chip can bind Human ITGA4&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-H52W1) with an affinity constant of 51.3 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
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FAQ
- product
Can lyophilized proteins remain stable during room-temperature shipping or temporary room-temperature exposure?
In general, lyophilized proteins remain stable during room-temperature shipping or temporary exposure to room-temperature conditions.
Many ACROBiosystems recombinant proteins are supplied in lyophilized (freeze-dried) form. The lyophilization process effectively removes moisture from the product, helping reduce degradation-related reactions and improve protein stability during transportation and storage.
To evaluate the stability of lyophilized proteins under normal temperature conditions, ACROBiosystems conducted a stability study on 11 representative lyophilized protein products at 37°C. The study demonstrated that these proteins maintained good quality stability after storage at 37°C for approximately 20 days. Based on these validation results, temporary room-temperature exposure during shipping or handling is generally not expected to have a significant impact on product quality or downstream application performance.
To ensure optimal long-term stability, products should be stored according to the storage conditions specified in the product Certificate of Analysis (COA) upon receipt.
Supporting Document
Background
Recent Advances
Drug Development Progress- English Name：
Integrin alpha-4/beta-1
- Category：
- Approved Drugs：
3 Details
- Drugs in Clinical Trials：
6 Details
- Highest Development Stage：
Approved
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