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Your Position: Home > All Other Proteins > Integrin alpha 4 beta 1 > IT1-H52W1

Human Integrin alpha 4 beta 1 (ITGA4&ITGB1) Heterodimer Protein, His Tag&Tag Free

  • Synonym
    Integrin alpha 4 beta 1,ITGA4&ITGB1
  • Source
    Human ITGA4&ITGB1 Heterodimer Protein, His Tag&Tag Free (IT1-H52W1) is expressed from human 293 cells (HEK293). It contains AA Tyr 34 - Thr 977 (ITGA4) & Gln 21 - Asp 728 (ITGB1) (Accession # P13612-1 (ITGA4) & P05556-1 (ITGB1)).
    Predicted N-terminus: Tyr 34 & Ser 592 (ITGA4) & Gln 21 (ITGB1)
  • Molecular Characterization
    Online(Tyr 34 - Thr 977 (ITGA4) & Gln 21 - Asp 728 (ITGB1)) P13612-1 (ITGA4) & P05556-1 (ITGB1)

    Human ITGA4&ITGB1 Heterodimer Protein, His Tag&Tag Free, produced by co-expression of ITGA4 and ITGB1, has a calculated MW of 111.4 kDa (ITGA4) and 83.7 kDa (ITGB1). Subunit ITGA4 is fused with an acidic tail at the C-terminus and followed by a polyhistidine tag and subunit ITGB1 contains no tag but a basic tail at the C-terminus. The ITGA4 subunit is composed of a heavy chain (Tyr 34 - Arg 591, calculated MW 61.1 kDa) and a light chain (Ser 592 - Thr 977, calculated MW 50.1 kDa). Consequently ITGA4 migrates as 60-70 kDa, 80-85 kDa and 135-150 kDa, and ITGB1 as 100-133 kDa respectively under reducing (R) condition due to cleavage and glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Human ITGA4&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-H52W1) SDS-PAGE gel

Human ITGA4&ITGB1 Heterodimer Protein, His Tag&Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
Human ITGA4&ITGB1 Heterodimer Protein, His Tag&Tag FreeHuman ITGA4&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-H52W1) ELISA bioactivity

Immobilized Natalizumab at 1 μg/mL (100 μL/well) can bind Human ITGA4&ITGB1 Heterodimer Protein, His Tag&Tag Free (Cat. No. IT1-H52W1) with a linear range of 1-16 ng/mL (QC tested).

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Price(USD) : $2880.00

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