Immobilized Biotinylated Human CD73, His,Avitag (Cat. No. CD3-H82E3) at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate, can bind Monoclonal Anti-Human CD73 Antibody, Human IgG1 with a linear range of 0.3-2 ng/mL (Routinely tested).
5'-nucleotidase (5'-NT), also known as ecto-5'-nucleotidase or CD73 (cluster of differentiation 73), is an enzyme that is encoded by the NT5E gene. CD73 commonly serves to convert AMP to adenosine. Ecto-5-prime-nucleotidase (5-prime-ribonucleotide phosphohydrolase) catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate. Rare allelic variants are associated with a syndrome of adult-onset calcification of joints and arteries (CALJA) affecting the iliac, femoral, and tibial arteries reducing circulation in the legs and the joints of the hands and feet causing pain.
Triple negative breast cancer, Non small cell lung cancer (NSCLC), Renal cell carcinoma, Endometrial cancer, Squamous cell carcinoma of head and neck cancer (SCCHN), Colorectal cancer, Metastatic castration-resistant prostate cancer (mCRPC), Sarcoma, Bladder cancer, Ovarian cancer, Cervical carcinoma, Pancreatic cancer