Save Product
Product Details
Synonyms
IL13, ALRH, BHR1, MGC116786, MGC116788, MGC116789, P600, Interleukin-13
Source
Biotinylated Human IL-13, His,Avitag (IL3-H82E5) is expressed from human 293 cells (HEK293). It contains AA Gly 21 - Asn 132 (Accession # AAK53823.1).
Predicted N-terminus: Gly 21
Request for sequenceMolecular Characterization
Other Tags and Version Biotin & Other Labeled Version
This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).
The protein has a calculated MW of 15.9 kDa. The protein migrates as 14-15 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Labeling
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Protein Ratio
Passed as determined by the HABA assay / binding ELISA.
Purity
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Storage
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
ACRO Quality Management System
Customers Also Viewed
Performance Data
SDS-PAGE
Biotinylated Human IL-13, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).
SEC-MALS
The purity of Biotinylated Human IL-13, His,Avitag (Cat. No. IL3-H82E5) is more than 90% and the molecular weight of this protein is around 14-20 kDa verified by SEC-MALS.
Report
Bioactivity-ELISA
Immobilized Human IL-13 R alpha 2, Fc Tag (Cat. No. IL2-H5256) at 5 μg/mL (100 μL/well) can bind Biotinylated Human IL-13, His,Avitag (Cat. No. IL3-H82E5) with a linear range of 0.02-1 ng/mL (QC tested).
Protocol
Immobilized Biotinylated Human IL-13, His,Avitag (Cat. No. IL3-H82E5) at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate can bind Monoclonal Anti-Human IL-13 Antibody, Human IgG4 with a linear range of 0.2-2 ng/mL (Routinely tested).
Protocol
Bioactivity-SPR
Human IL-13 R alpha 2 Protein, Fc Tag (Cat. No. IL2-H5256) captured on Protein A Chip can bind Biotinylated Human IL-13, His,Avitag (Cat. No. IL3-H82E5) with an affinity constant of 10.1 pM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Monoclonal Anti-Human IL-13 Antibody, Human IgG4 captured on Protein A Chip can bind Biotinylated Human IL-13, His,Avitag (Cat. No. IL3-H82E5) with an affinity constant of 0.106 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Human IL-13 R alpha 1 Protein, His Tag (Cat. No. IL1-H5224) immobilized on CM5 Chip can bind Biotinylated Human IL-13, His,Avitag (Cat. No. IL3-H82E5) with an affinity constant of 52.7 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
Protocol
Bioactivity-CELL BASE
Biotinylated Human IL-13, His,Avitag (Cat. No. IL3-H82E5) induced STAT6 Reporter activity in HEK293 cells. The EC50 value of this effect is 0.027 μg/mL (Routinely tested).
Protocol
Customer Reviews Writing Reviews
Background
Recent Advances
Drug Development Progress- English Name：
Interleukin-13
- Category：
- Approved Drugs：
4 Details
- Drugs in Clinical Trials：
27 Details
- Highest Development Stage：
Approved
Contact Us
[North America]: +1 800-810-0816
[Switzerland]: +41 800 040 012
[Asia & Pacific]: +86 400-682-2521
Drug Candidate Licensing
