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Your Position: Home > Kits > S protein > TAS-K007

Anti-SARS-CoV-2 Antibody IgG Titer Serologic Assay kit (S protein trimer)

For research use only.
Materials Provided
IDCompositionSize
TAS001-C01High-bind Plate96 tests
TAS007-C02SARS-CoV-2 S protein30 μg
TAS007-C03Anti-SARS-CoV-2 Antibody (Control, IgG)10 μg
TAS001-C04HRP-Anti-Human IgG10 μg
TAS001-C05Blocking / Dilution Buffer60mL
  • Background
    The newly identified Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) has posed a serious threat to human health. A rapid and effective assay kit detecting the levels of anti-SARS-CoV-2 in human serum can facilitate research on characterization of antibodies produced in response to SARS-CoV-2 infection.
  • Application
    This kit is developed for serologic test for IgG titer of Anti-SARS-CoV-2 antibody in serum/plasma in vitro.
    It is for research use only.
  • Reconstitution
    Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
  • Storage

    Upon receipt, please store the lyophilized products at -20℃. After reconstitution, the stock solution should be kept at -70℃. Upon receipt, please store the plate at Room Temperature, and please store the buffer at -20℃.

    It is recommended not to freeze thaw more than 3 times.

    This product is stable under storage conditions: 12 months in sealed state; Used within 3 months after opening.
  • Assay Principles
    This assay kit employs a standard indirect-ELISA format, providing a rapid detection of anti-SARS-CoV-2 IgG in serum by SARS-CoV-2 S Protein. The kit consists of High-bind Plate, S Protein, an Anti-SARS-CoV-2 Antibody (Control, IgG), an HRP-Anti-Human IgG secondary antibody and Blocking/Dilution buffer.

    Your experiment will include 6 simple steps:

    a) Coat the plate with SARS-CoV-2 S Protein.

    b) Wash the plate with universal ELISA buffer and block the plate with the blocking buffer.

    c) Add your sample to the plate, take the Anti-SARS-CoV-2 antibody as Control sample. The samples and Control sample are diluted by Blocking / Dilution Buffer.

    d) Add a diluted Secondary antibody HRP-Anti-Human IgG to the plate. The Secondary antibody is diluted by Blocking / Dilution Buffer.

    e) Wash the plate and add TMB or other colorimetric HRP substrate.

    f) Stop the substrate reaction by add diluted acid. Absorbance (OD) is calculate as the absorbance at 450 nm minus the absorbance at 650 nm to remove background prior to statistical analysis. The OD Value reflects the amount of antibody bound.

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Price(USD) : $480.00

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