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Your Position: Home > Viral Proteins > Spike S2 > S2N-C52H2

SARS-CoV-2 (COVID-19) S2 protein, His Tag

This product is specifically designed for serological test development.
Please contact us if you are interested in another version which is designed for antibody drug development.
INTRODUCTION
QC & Assay Protocol
Citation & Background
  • Synonym
    Spike,S2 protein,Spike glycoprotein Subunit2,S glycoprotein Subunit2,Spike protein S2
  • Source
    SARS-CoV-2 S2 protein, His Tag (S2N-C52H2) is expressed from human 293 cells (HEK293).
    Request for sequence
  • Molecular Characterization

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 60.0 kDa. The protein migrates as 65-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Supplied as 0.2 μm filtered solution in 20 mM PB, 300 mM NaCl, pH7.4 with glycerol as protectant.

    Contact us for customized product form or formulation.

  • Storage

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -70°C or lower upon receipt;
    2. -70°C for 3 months under sterile conditions.
  • Shipping
    This product is supplied and shipped as sterile liquid solution with dry ice, please inquire the shipping cost.
SDS-PAGE
SARS-CoV-2 S2 protein, His Tag (Cat. No. S2N-C52H2) SDS-PAGE gel

SARS-CoV-2 S2 protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
SARS-CoV-2 S2 protein, His TagSARS-CoV-2 S2 protein, His Tag (Cat. No. S2N-C52H2) ELISA bioactivity

Immobilized SARS-CoV-2 S2 protein, His Tag (Cat. No. S2N-C52H2) at 1 μg/mL (100 μL/well) can bind SARS-CoV-2 Spike S2 Antibody, Human IgG1 with a linear range of 0.1-3 ng/mL (QC tested).

Protocol
  • Citations
  • Background
    It's been reported that SARS-CoV-2 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
  • Clinical and Translational Updates
      

  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

  • Purchaser Notification
    Important Licensing Information: This product may be covered by Limited Use Label License(s). By use of this product, you accept the terms and conditions of all applicable Limited Use Label License(s).
  • Limited Use Label License No. 18: Engineered Coronavirus Spike Proteins
    This product and its use is the subject of U.S. Provisional Patent Application No. 63/032,502 and sold under a license from The University of Texas at Austin. Rights to use this product are limited to research and clinical diagnostics, and expressly exclude the right to use in human therapeutics, vaccines, and any use involving the administration of this product to humans.

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