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Your Position: Home > Kits > SARS-CoV-2 RBD > TAS-K022

Anti-SARS-CoV-2 Neutralizing Antibody Titer Serologic Assay Kit

For research use only.
Materials Provided
IDComponentsSize
TAS022-C01Pre-coated with Human ACE2 Protein Microplate1 plate
TAS022-C02Anti-SARS-CoV-2 Neutralizing Antibody10 μg
TAS022-C03HRP-SARS-CoV-2 Spike RBD10 μg
TAS022-C0410xWashing Buffer50 mL
TAS022-C05Dilution Buffer50 mL
TAS022-C06Substrate Solution12 mL
TAS022-C07Stop Solution7 mL
  • Background
    The newly identified Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) poses continual threat to human health due to rapid transmission worldwide. The Anti SARS-CoV-2 Neutralizing Antibody Titer Serologic Assay Kit to test the protective neutralizing antibody levels in post-vaccination serum or convalescent serum.
  • Application
    This assay kit is used to measure the levels of Anti-SARS-CoV-2 neutralizing antibody which interfere ACE2/Spike RBD interaction.
    It is for research use only.
  • Reconstitution
    Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
  • Storage

    The unopened kit is stable for at least 1 year from the date of manufacture if stored at 2°C to 8°C, and the opened kit is stable for up to 1 month from the date of opening at 2°C to 8°C.

    It is recommended not to freeze thaw more than 3 times for lyophilized proteins.

  • Assay Principles
    The Anti-SARS-CoV-2 Neutralizing Antibody Titer Serologic Assay kit is a competitive ELISA. Immobilized Human ACE2 protein on the microplate are incubated with samples and Spike RBD-HRP. Presence of anti-SARS-CoV-2 neutralizing antibody in the samples competes with immobilized Human ACE2 protein to bind Spike RBD-HRP. The intensity of assay signal decreases proportionally to the presence of Anti-SARS-CoV-2 neutralizing antibody.

    The kit analysis includes 5 simple steps:

    a) All reagents were returned to room temperature(20℃-25℃) before use.

    b) Series of sample and control antibody dilution are prepared with dilution buffer. Solution of Spike RBD-HRP is prepared with dilution buffer.

    c) The sample dilution is added to plate followed by Spike RBD-HRP solution. For semiquantitative analysis, dilution of control neutralizing antibody is add to the plate followed by Spike RBD-HRP solution.

    d) Wash the plate and add TMB or other colorimetric HRP substrate.

    e) The substrate reaction is stopped by add diluted acid. Absorbance (OD) is calculated as the absorbance at 450 nm minus the absorbance at 650 nm to remove background noise prior to statistical analysis. The OD Value reflects the amount of protein bound.

Typical Data
Anti-SARS-CoV-2 neutralizing antibody titer serologic assay kitAnti-SARS-CoV-2 neutralizing antibody titer serologic assay kit (Cat. No. TAS-K022) ELISA bioactivity

Inhibition of Human ACE2: SARS-CoV-2 Spike RBD (HRP) binding by Anti-SARS-CoV-2 Neutralizing Antibody measured by competitive ELISA Assay.
Serial dilutions of Anti-SARS-CoV-2 Neutralizing Antibody (Cat. No. TAS022-C02) (1:2 serial dilutions, from 1.25 μg/mL to 0.0012 μg/mL) was added into Human ACE2: SARS-CoV-2 Spike RBD (HRP) binding reactions. The assay was performed according to the above described protocol. Background was subtracted from data points prior to log transformation and curve fitting. A representative result showed that the IC50 was calculated to be 0.06 μg/mL (QC tested).

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Price(USD) : $480.00

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