|TAS022-C01||Pre-coated with Human ACE2 Protein Microplate||1 plate|
|TAS022-C02||Anti-SARS-CoV-2 Neutralizing Antibody||10 μg|
|TAS022-C03||HRP-SARS-CoV-2 Spike RBD||10 μg|
|TAS022-C04||10xWashing Buffer||50 mL|
|TAS022-C05||Dilution Buffer||50 mL|
|TAS022-C06||Substrate Solution||12 mL|
|TAS022-C07||Stop Solution||7 mL|
The unopened kit is stable for at least 1 year from the date of manufacture if stored at 2°C to 8°C, and the opened kit is stable for up to 1 month from the date of opening at 2°C to 8°C.
It is recommended not to freeze thaw more than 3 times for lyophilized proteins.
The kit analysis includes 5 simple steps:
a) All reagents were returned to room temperature(20℃-25℃) before use.
b) Series of sample and control antibody dilution are prepared with dilution buffer. Solution of Spike RBD-HRP is prepared with dilution buffer.
c) The sample dilution is added to plate followed by Spike RBD-HRP solution. For semiquantitative analysis, dilution of control neutralizing antibody is add to the plate followed by Spike RBD-HRP solution.
d) Wash the plate and add TMB or other colorimetric HRP substrate.
e) The substrate reaction is stopped by add diluted acid. Absorbance (OD) is calculated as the absorbance at 450 nm minus the absorbance at 650 nm to remove background noise prior to statistical analysis. The OD Value reflects the amount of protein bound.
Inhibition of Human ACE2: SARS-CoV-2 Spike RBD (HRP) binding by Anti-SARS-CoV-2 Neutralizing Antibody measured by competitive ELISA Assay.
Serial dilutions of Anti-SARS-CoV-2 Neutralizing Antibody (Cat. No. TAS022-C02) (1:2 serial dilutions, from 1.25 μg/mL to 0.0012 μg/mL) was added into Human ACE2: SARS-CoV-2 Spike RBD (HRP) binding reactions. The assay was performed according to the above described protocol. Background was subtracted from data points prior to log transformation and curve fitting. A representative result showed that the IC50 was calculated to be 0.06 μg/mL (QC tested).
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High quality SARS-CoV-2 S protein, S1 protein and human ACE2 protein based on the human cell expression platform of HEK293.
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