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Your Position: Home > Cell > Fc gamma RIIA / CD32a > SCCHO-ATP061M

CHO/Human CD32a Stable Cell Line (Medium Expression) Development Service

  • Description
    The CHO/Human CD32a Stable Cell Line was engineered to express full length human CD32a receptor (Gene ID: 2212), with different levels of CD32a expression (High, Medium, Low), which can be used to test agonist antibody whether in a CD32a-dependent manner to strengthen the agonistic activity. When co-cultured with Human 4-1BB (Luc) HEK293 Reporter Cell (Cat.No.CHEK-ATF073) and anti-4-1BB agonist antibody, the anti-4-1BB antibody can be crosslinked, thereby strengthening 4-1BB pathway-activated luminescence.
  • Application

    • Useful for cell-based CD32a binding assay

    • Useful for CD32a-mediated crosslinking in reporter gene assay

     Fc gamma RIIA / CD32a Assay Principles

  • Growth Properties
  • Selection Marker
    Hygromycin (20 μg/mL)
  • Culture Medium
    F-12K+ 10% FBS
  • Freeze Medium
    Serum-free cell cryopreservation medium
  • Quantity
    1 vial contains at least 5×10^6 cells in 1 mL serum-free cryopreservation medium
  • Storage
    Frozen in liquid nitrogen.
  • Instructions for Use
    See data sheet for detailed culturing and assay protocol.
Receptor Assay
 Fc gamma RIIA / CD32a FACS

Expression analysis of human CD32a on CHO/Human CD32a Stable Cell Line by FACS.
Cell surface staining using PE-labeled anti-human CD32a antibody was performed on CHO/Human CD32a Stable Cell Line with different expression levels: CHO/Human CD32a Stable Cell Line (Low Expression); CHO/Human CD32a Stable Cell Line (Medium Expression); CHO/Human CD32a Stable Cell Line (High Expression).


Bioactivity analysis of anti-human 4-1BB antibody through CHO/Human CD32a Stable Cell Line (Medium Expression) crosslinking to test whether in a CD32a-dependent manner to strengthen the agonistic activity.
The EC50 of anti-human 4-1BB antibody is approximately 0.05 μg/mL through CHO/Human CD32a Stable Cell Line (Medium Expression) crosslinking.

  • Background
    Receptors for the Fc region of IgG (Fc γ R) are members of the Ig superfamily that function in the activation or inhibition of immune responses. Three classes of human Fc γ Rs: RI (CD64), RII (CD32), and RIII (CD16), which generate multiple isoforms, are recognized. There are three genes for human Fcγ RII /CD32 (A, B, and C) and one for mouse Fcγ RII B (CD32B). CD32 is a low affinity receptor for IgG. The activating isoform, CD32A, is expressed on monocytes, neutrophils, platelets and dendritic cells. CD32A is expressed on many immune cell types (macrophage, neutrophil, eosinophils, platelets, dendritic cells and Langerhan cells), where inhibitory ITIM­bearing receptors may also be coexpressed and co­engaged by specific ligands. CD32A delivers an activating signal upon ligand binding, and results in the initiation of inflammatory responses including cytolysis, phagocytosis, degranulation and cytokine production. The responses can be modulated by signals from the coexpressed inhibitory receptors such as CD32B, and the strength of the signal is dependent on the ratio of expression of the activating and inhibitory receptors.
  • Clinical and Translational Updates
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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ETD of in-stock products: 2 business days

Price(USD) : $3150.00

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Drug Development Status

  • Number of Launched Drugs:0 Details
  • Number of Drugs in Clinical Trials:1 Details
  • Latest Research Phase:Phase 1 Clinical

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