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Your Position: Home > Kits > - > RIPO-HWM002K

Human iPSC-Derived Cardiac Organoid differentiation Kit

For research use only.

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Materials Provided
Box A(2-8°C)
IDComponentsSize
RIPO-HWM002K-C01Basal medium A5.4ml
RIPO-HWM002K-C02Basal medium B22.5ml
RIPO-HWM002K-C03Basal medium C27ml
RIPO-HWM002K-C04Basal medium M-M22.5ml
 
Box B(-20°C)
IDComponentsSize
RIPO-HWM002K-1-C01Medium F3ml
RIPO-HWM002K-1-C02Supplement A0.6ml
RIPO-HWM002K-1-C03Supplement B2.5ml
RIPO-HWM002K-1-C04Supplement C3ml
RIPO-HWM002K-1-C05Supplement M-M2.5ml
  • Product Details
    Human iPSC-Derived Cardiac Organoid differentiation Kit (Catalog No#: RIPO-HWM002K) allows hESC or iPSC to differentiate into cardiac organoids. Cardiac organoids are three-dimensional in vitro models with a cellular composition and structural organization that is representative of the human heart. This kit can produce 24 cardiac organoids in four steps. Differentiation was carried out by forming EBs from PSC at an ultra-low adsorption interface, and then changing the medium according to the instructions. A spontaneously beating cardiac organoid with a cavity can be seen between days 9 and 13 of the onset of differentiation. Cardiomyocyte, key cellular component of cardiac organoid, are responsible for the beating of the cardiac organoids. The beating rate of the formed cardiac organoids increases dramatically when stimulated with isoproterenol or forskolin, and there is a linear relationship between the concentration of the drug and the beating rate over a range of concentrations.
  • Product Specification
    The basic medium of this differentiation kit is a serum-free, well-defined medium with minimal batch variation to which differentiation factors are added. This medium does not contain antibiotics, the addition of which may affect cardiac organoid differentiation.
  • Storage

    The unopened Box A is stable for 12 months from the date of manufacture if stored under 2-8°C.
    The unopened Box B is stable for 12 months from the date of manufacture if stored under -20°C.
    The opened kit should be stored according to components table.

Product Diagram
 - PRODUCT DIAGRAM

Protocol Diagram of cardiac organoid differentiation.

Validation Data
Organoid Morphology and State
 - Organoid Morphology And State

The cardiac organoids differentiated using the Human iPSC-Derived Cardiac Organoid Differentiation Kit (Ca. No. RIPO-HWM002K) show regular beating from day 7-13 of differentiation.

Marker Expression
 - MARKER EXPRESSION

The cardiac organoids differentiated using the Human iPSC-Derived Cardiac Organoid Differentiation Kit (Ca. No. RIPO-HWM002K) demonstrate the expression of cardiomyocyte marker cTnT (green).

Organoid Activity
 - ORGANOID ACTIVITY

The electrical activity of the cardiac organoids differentiated using the Human iPSC-Derived Cardiac Organoid Differentiation Kit (Ca. No. RIPO-HWM002K) was recorded using silicon probes (Ca. No. P64-7), revealing electrophysiological activity that closely resembles that of the actual human heart.

  • Clinical and Translational Updates

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