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Product Details
Synonyms
FLJ18683, T3E, TCRE, CD3E, CD3-epsilon
Source
Alexa Fluor 488-Labeled Human CD3 epsilon Protein, His Tag (CDE-HA2H3) is expressed from human 293 cells (HEK293). It contains AA Asp 23 - Asp 126 (Accession # P07766-1). It is the Alexa Fluor™ 488 labeled form of Human CD3 epsilon, His Tag (Cat. No. CDE-H5223).
Predicted N-terminus: Asp 23
Molecular Characterization
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 16.9 kDa. The protein migrates as 20-25 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Conjugate
AF488
Excitation Wavelength: 488 nm
Emission Wavelength: 517 nm
Labeling
The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with AF488 using standard chemical labeling method. The residual AF488 is removed by molecular sieve treatment during purification process.
Protein Ratio
The AF488 to protein molar ratio is 1-2.
Purity
>90% as determined by SDS-PAGE.
Formulation
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Reconstitution
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
Shipping and Storage
This product is shipped at ambient temperature.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please protect from light and avoid repeated freeze-thaw cycles.
This product is stable after storage at:
- -20°C to -70°C for 12 months in lyophilized state;
- -70°C for 3 months under sterile conditions after reconstitution.
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Performance Data
SDS-PAGE
Alexa Fluor 488-Labeled Human CD3 epsilon Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
Bioactivity-ELISA
Immobilized Alexa Fluor 488-Labeled Human CD3 epsilon Protein, His Tag (Cat. No. CDE-HA2H3) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Human CD3 Antibody, Mouse IgG1 (SP34-2) (Cat. No. CDE-M531) with a linear range of 0.2-8 ng/mL (QC tested).
Protocol
Bioactivity-Flow Cytometry
1e5 of Mouse Anti-CD3 antibody coupled beads (5.5μm) were stained with different concentration of Alexa Fluor 488-Labeled Human CD3 epsilon Protein, His Tag (Cat. No. CDE-HA2H3) and negative control protein respectively, AF488 signal was used to evaluate the binding activity (QC tested).
Protocol
1e5 of Mouse Anti-CD3 antibody coupled beads (5.5μm) were stained with different concentration of Alexa Fluor 488-Labeled Human CD3 epsilon Protein, His Tag (Cat. No. CDE-HA2H3) and negative control protein respectively, AF488 signal was used to evaluate the binding activity (QC tested).
Protocol
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FAQ
- product
Can lyophilized proteins remain stable during room-temperature shipping or temporary room-temperature exposure?
In general, lyophilized proteins remain stable during room-temperature shipping or temporary exposure to room-temperature conditions.
Many ACROBiosystems recombinant proteins are supplied in lyophilized (freeze-dried) form. The lyophilization process effectively removes moisture from the product, helping reduce degradation-related reactions and improve protein stability during transportation and storage.
To evaluate the stability of lyophilized proteins under normal temperature conditions, ACROBiosystems conducted a stability study on 11 representative lyophilized protein products at 37°C. The study demonstrated that these proteins maintained good quality stability after storage at 37°C for approximately 20 days. Based on these validation results, temporary room-temperature exposure during shipping or handling is generally not expected to have a significant impact on product quality or downstream application performance.
To ensure optimal long-term stability, products should be stored according to the storage conditions specified in the product Certificate of Analysis (COA) upon receipt.
Supporting Document
Background
Recent Advances
Drug Development Progress- English Name：
T-cell surface glycoprotein CD3 epsilon chain
- Category：
- Approved Drugs：
4 Details
- Drugs in Clinical Trials：
7 Details
- Highest Development Stage：
Approved
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