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Your Position: Home > All Other Proteins > uPAR > UPR-H5226

Human uPAR / PLAUR Protein, His Tag

  • Synonym
  • Source
    Human uPAR, His Tag (UPR-H5226) is expressed from human 293 cells (HEK293). It contains AA Leu 23 - Arg 303 (Accession # AAH02788).
    Predicted N-terminus: Leu 23
  • Molecular Characterization
    Online(Leu 23 - Arg 303) AAH02788

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 32.3 kDa. The protein migrates as 40-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >92% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
Human uPAR, His Tag (Cat. No. UPR-H5226) SDS-PAGE gel

Human uPAR, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 92%.

Human uPAR, His TagHuman uPAR, His Tag (Cat. No. UPR-H5226) ELISA bioactivity

Measured by its inhibitory ability in a competitive ELISA.
Serial dilutions of Human uPAR, His Tag (Cat. No. UPR-H5226) were added into Human PLAU, His Tag (Cat. No. PLU-H5229) : Biotinylated Human uPAR, His,Avitag (Cat. No. UPR-H82E7) binding reactions. The half maximal inhibitory concentration (IC50) is 2.96 μg/mL (QC tested).

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