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Full Length CD20 Protein

ELISA/ SPR Validated and Protocol Offered

PD1 Pathway
Background

CD20 is a hot target for the treatment of lymphoma, leukemia and some autoimmune diseases. However, due to low expression titer and the unstable bioactivity, it is so difficult to find an active CD20 protein on the market for antibody drug development, quality control, and evaluation of CD20-CAR expression level.

To address these issues, ACROBiosystems has developed the highly active full length multi-transmembrane CD20 proteins, expressed by HEK293 cells and insect cells, respectively. The active full length CD20 proteins carry both big and small ECD loops, and bind Rituximab and Ofatumumab with high binding affinity in ELISA and SPR assay, which are suitable for ELISA, SPR, BLI and immunization assays.

Application
Product List
MoleculeCat. No.HostProduct Description
CD20/ MS4A1CD0-H52H3HEK293Human CD20/ MS4A1 Full Length Protein, His Tag
CD0-H55Ha Insect cells-HI5Human CD20/ MS4A1 Full Length Protein, His Tag
Product Features

Full length CD20 protein has a complete conformation with both small and big loops.

Full length CD20 protein has better bioactivity

A) HEK293 Cell (Full Length)

Immobilized Rituximab at 2 μg/mL (100 μL/well) can bind Human CD20 Full Length, His Tag, HEK293 (Cat. No. CD0-H52H3) with a linear range of 2-16 ng/mL.

B) Insect Cell (Full Length)

Immobilized Rituximab at 2 μg/mL (100 μL/well) can bind Human CD20 Full Length, His Tag, Insect cell (Cat. No. CD0-H55Ha) with a linear range of 1-16 ng/mL.

C) E.coli (Extracellular)

Immobilized Rituximab at 5 μg/mL (100 μL/well) can bind Human CD20, TrxA Tag, His Tag (E.coli) with a linear range of 78-2500 ng/mL.

The bioactivity of full length CD20 protein was verified by ELISA/ SPR and the protocols are offered.

ELISA
Related CD20 proteins: Cat. No. CD0-H52H3 (HEK293 expression) & Cat. No. CD0-H55Ha (Insect cells expression)

Immobilized Ofatumumab at 10 μg/mL (100 μL/well) can bind Human CD20 Full Length, His Tag, HEK293 (Cat. No. CD0-H52H3) with a linear range of 2-31 ng/mL.


Immobilized Human CD20 Full Length, His Tag, Insect cell (Cat. No. CD0-H55Ha) at 2 μg/mL (100 μL/well) on Nickel Coated Plate, can bind rituximab with a linear range of 0.313-1.25 μg/mL.

Immobilized Ofatumumab (Human IgG1) at 10 μg/mL (100 μL/well) can bind Human CD20 Full Length, His Tag, Insect cell (Cat. No. CD0-H55Ha) with a linear range of 2-31 ng/mL.


SPR
Related CD20 proteins: Cat. No. CD0-H52H3 (HEK293 expression) & Cat. No. CD0-H55Ha (Insect cells expression)

MabThera® (Rituximab) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human CD20 Full Length, His Tag, HEK293 (Cat. No. CD0-H52H3) with an affinity constant of 1.77 nM as determined in an SPR assay (Biacore T200).

Ofatumumab (Human IgG1) captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human CD20 Full Length, His Tag, HEK293 (Cat. No. CD0-H52H3) with an affinity constant of 3.57 nM as determined in an SPR assay (Biacore T200).

MabThera® (Rituximab) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind Human CD20 Full Length, His Tag, Insect cell (Cat. No. CD0-H55Ha) with an affinity constant of 1.21 nM as determined in an SPR assay (in presence of DDM) (Biacore T200).

Ofatumumab (Human IgG1) captured on CM5 chip via ant-human IgG Fc antibodies surface can bind Human CD20 Full Length, His Tag, Insect cell (Cat. No. CD0-H55Ha) with an affinity constant of 2.977 nM as determined in an SPR assay (Biacore T200).

If you would like ACRO to add more experimental design and application data of this product, please contact us.

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