A-Z> CD73 A novel target for immunotherapy
CD73 is known as ecto-5-prime-nucleotidase, the enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol (GPI) linkage to the external face of the plasma membrane. The enzyme is used as a marker of lymphocyte differentiation. CD73 works with its upstream signaling molecule CD39 to convert extracellular ATP to adenosine. Adenosine in tumor microenvironment can help regulatory T cells suppress and shut down immune activity. Tumor cells can also express CD73 which can promote the release of adenosine, thereby reducing antitumor activity.
An article[1] published in Nature Medicine identified CD73 as a specific immunotherapeutic target to improve antitumor immune responses to immune checkpoint therapy in glioblastoma multiforme. The researchers pointed out that anti-CD73 antibodies have achieved good results in preclinical and early clinical studies, and proposed a strategy for CD73, PD-1 and CTLA-4 blocking combined therapy for glioblastoma.
ACROBiosystems provides CD73 proteins with high enzyme activity, which can help expedite your anti-CD73 mAb drug development. The high purity and bioactivity of these CD73 proteins have been verified by HPLC, BLI, SPR and ELISA. Protocols are provided for free.
| Cat. No. | Species | Tag |
|---|---|---|
| CD3-H52H7 | Human | His tag |
| CD3-H82E3 | Human | His Tag & Avi Tag |
| CD3-M52H9 | Mouse | His tag |
Measured by its ability to hydrolyze the 5’-phosphate group from the substrate adenosine-5’-monophosphate (AMP). The specific activity is > 37,000 pmol/min/μg.
The purity of CD73 proteins are verified by SEC-HPLC.
Fig.1 The purity of Human CD73, His Tag (HPLC-verified) (Cat. No. CD3-H52H7) was greater than 90% as determined by SEC-HPLC.
The bioactivity of CD73 proteins are verified by BLI, SPR and ELISA, and the protocols are offered.
Fig.2 Loaded Anti-CD73 MAb (Human IgG1) on AHC Biosensor, can bind Human CD73, His Tag (Cat. No.CD3-H52H7) with an affinity constant of 0.179 nM as determined in BLI assay (ForteBio Octet Red96e).
Fig.3 Anti-CD73 antibody (Human IgG1) captured on CM5 chip via anti-human IgG Fc antibodies surface can bind Human CD73, His Tag, HEK293 (Cat. No. CD3-H52H7) with an affinity constant of 0.164 nM as determined in SPR assay (Biacore 8K).
Fig.4 Immobilized Human CD73, His Tag (HPLC-verified) (Cat. No. CD3-H52H7) at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-Human CD73 Antibody, Human IgG1 with a linear range of 0.15-5 ng/mL.
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