Cytokine Release Detection Kits
ACROBiosystems has independently developed a series of cytokine detection kits based on the Cytometric Bead Array Platform. Rigorously validated through comprehensive methodological studies, these assays enable accurate quantitative detection of single or multiple cytokines simultaneously using flow cytometry. Designed to support applications across preclinical research, clinical studies, and manufacturing quality control, they provide a comprehensive, efficient, and cost-effective solution to accelerate drug development workflows.
Cytometric Bead Array Platform Detection Principles：
1. Flow cytometry separates magnetic beads based on the fluorescent wavelength emitted by each bead. Specific antibodies are coupled to the surface of each bead which binds to a specific, different molecule.
2. PE-labeled detection antibodies are added to detect the molecules captured on the magnetic bead.
3. PE signal is detected by flow cytometry and quantified against a standard curve.
Principle diagram
Product Advantages
Internationally Standardized: Calibrated against NIBSC/WHO international reference standards.
Low sample volume: 10-30 uL per sample.
Fast results: quantitatively evaluate multiple cytokines in a single experiment.
Less steps: easy-to-perform protocol with less steps avoids magnetic bead loss.
Wide detection range: 4 order magnitude standard curve.
Universally compatible: adaptable to various flow cytometers and software.
Protein signal-to-noise ratio was used to screen antibody pairs to ensure performance. Using antibodies with the required sensitivity, cell supernatant, serum, plasma and other samples were used to determine the specific recognition of antibodies to real samples
Magnetic beads are used as the carrier to bind antibodies to the magnetic beads through a chemical reaction. After coupling, antibody coupling amounts were evaluated for consistency between batches and validated.
Non-specific signals from biotin and avidin are reduced by directly labeling by PE.
PBS is used to adjust the concentration to meet the interference level of common interfering substances in the kit samples, such as EDTA and hemoglobin.
Multiplex Cytokine Detection
Flow Cytometry–Based Multiplex Cytokine Assay Reagents
We offer a comprehensive range of fluorescently labeled magnetic beads, antibodies, and related reagents specifically designed for cytokine detection. These reagents enable the simultaneous quantitative measurement of multiple cytokines — including IL-2, IL-4, IL-6, IL-10, IL-15, IL-17A, TNF-α, and IFN-γ — in cell culture supernatants and serum samples. The platform supports cytokine profiling across Th1, Th2, Th17, and other immune cell subsets, significantly improving research efficiency and maximizing data acquisition from precious or limited samples.
Through rigorous methodological validation, we have independently developed the Th1/Th2 Cytokine Detection Kit (Catalog No. FCM-C05R) based on our proprietary flow cytometry multi-factor detection technology platform. This kit allows for the simultaneous quantitative detection of IFN-γ, TNF-α, IL-2, IL-4, IL-6, and IL-10 in cell culture media, serum, and plasma.
FAQ
- Background
- Product Advantages
- Multiplex Cytokine Detection
- FAQ
Online Message
[North America]: +1 800-810-0816
[Switzerland]: +41 800 040 012
[Asia & Pacific]: +86 400-682-2521
[North America]: 1 Innovation Way, Newark, DE 19711, USA
[Switzerland]: ACRObiosystems AG, c/o Zug Dammstrasse Centre AG, Dammstrasse 19, 6300 Zug Switzerland