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Your Position: Home > Immune Checkpoint Proteins > CTLA-4 > CT4-H52A4

Human CTLA-4 / CD152 Protein, mouse IgG2a Fc Tag, low endotoxin

  • Synonym
    CTLA4,CD152
  • Source
    Human CTLA-4, mouse IgG2a Fc tag, low endotoxin (CT4-H52A4) is expressed from human 293 cells (HEK293). It contains AA Ala 37 - Phe 162 (Accession # NP_005205.2).
    Predicted N-terminus: Ala 37
  • Molecular Characterization
    Online(Ala 37 - Phe 162) NP_005205.2

    This protein carries a mouse IgG2a Fc tag at the C-terminus. The protein has a calculated MW of 40.6 kDa. The protein migrates as 50 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation and 100 kDa under non-reducing (NR) condition.

  • Endotoxin
    Less than 0.1 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;

    2. -70°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE
Human CTLA-4, mouse IgG2a Fc tag, low endotoxin (Cat. No. CT4-H52A4) SDS-PAGE gel

Human CTLA-4, mouse IgG2a Fc tag, low endotoxin on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Human CTLA-4, mouse IgG2a Fc tag, low endotoxinHuman CTLA-4, mouse IgG2a Fc tag, low endotoxin (Cat. No. CT4-H52A4) ELISA bioactivity

Immobilized Human B7-1, Fc Tag (Cat. No. B71-H5259) at 2 μg/mL (100 μL/well) can bind Human CTLA-4, mouse IgG2a Fc tag, low endotoxin (Cat. No. CT4-H52A4) with a linear range of 0.1-2 ng/mL (QC tested).

Human CTLA-4, mouse IgG2a Fc tag, low endotoxinHuman CTLA-4, mouse IgG2a Fc tag, low endotoxin (Cat. No. CT4-H52A4) ELISA bioactivity

Immobilized Human B7-2, Fc Tag (Cat. No. CD6-H5257) at 2 μg/mL (100 μL/well) can bind Human CTLA-4, mouse IgG2a Fc tag, low endotoxin (Cat. No. CT4-H52A4) with a linear range of 0.5-7.8 ng/mL (Routinely tested).

Human CTLA-4, mouse IgG2a Fc tag, low endotoxinHuman CTLA-4, mouse IgG2a Fc tag, low endotoxin (Cat. No. CT4-H52A4) ELISA bioactivity

Immobilized Ipilimumab at 5 μg/mL (100 μL/well) can bind Human CTLA-4, mouse IgG2a Fc tag, low endotoxin (Cat. No. CT4-H52A4) with a linear range of 0.1-0.8 ng/mL (Routinely tested).

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