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Your Position: Home > Immune Checkpoint Proteins > CTLA-4 > Human CTLA-4 / CD152 Protein, mouse IgG2a Fc Tag, low endotoxin

Human CTLA-4 / CD152 Protein, mouse IgG2a Fc Tag, low endotoxin

  • Synonym
    CTLA4,CD152
  • Source
    Human CTLA-4, mouse IgG2a Fc Tag, low endotoxin (CT4-H52A4) is expressed from human 293 cells (HEK293). It contains AA Ala 37 - Phe 162 (Accession # NP_005205.2).
    Predicted N-terminus: Ala 37
  • Molecular Characterization
    CTLA-4(Ala 37 - Phe 162) NP_005205.2

    This protein carries a mouse IgG2a Fc tag at the C-terminus. The protein has a calculated MW of 40.6 kDa. The protein migrates as 50 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation and 100 kDa under non-reducing (NR) condition.

  • Endotoxin
    Less than 0.1 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss was observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;

    2. -70°C for 3 months under sterile conditions after reconstitution.

SDS-PAGE
Human CTLA-4, mouse IgG2a Fc Tag, low endotoxin (Cat. No. CT4-H52A4) SDS-PAGE gel

Human CTLA-4, mouse IgG2a Fc Tag, low endotoxin on SDS-PAGE under reducing (R) and no-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Bioactivity-ELISA
Human CTLA-4, mouse IgG2a Fc Tag, low endotoxinHuman CTLA-4, mouse IgG2a Fc Tag, low endotoxin (Cat. No. CT4-H52A4) ELISA bioactivity

Immobilized Human B7-1, Fc Tag (Cat. No. B71-H5259) at 2 μg/mL (100 μL/well) can bind Human CTLA-4, mouse IgG2a Fc tag, low endotoxin (Cat. No. CT4-H52A4) with a linear range of 0.1-2 ng/mL (QC tested).

Human CTLA-4, mouse IgG2a Fc Tag, low endotoxinHuman CTLA-4, mouse IgG2a Fc Tag, low endotoxin (Cat. No. CT4-H52A4) ELISA bioactivity

Immobilized Human B7-2, Fc Tag (Cat. No. CD6-H5257) at 2 μg/mL (100 μL/well) can bind Human CTLA-4, mouse IgG2a Fc tag, low endotoxin (Cat. No. CT4-H52A4) with a linear range of 0.5-7.8 ng/mL (Routinely tested).

Human CTLA-4, mouse IgG2a Fc Tag, low endotoxinHuman CTLA-4, mouse IgG2a Fc Tag, low endotoxin (Cat. No. CT4-H52A4) ELISA bioactivity

Immobilized Ipilimumab at 5 μg/mL (100 μL/well) can bind Human CTLA-4, mouse IgG2a Fc tag, low endotoxin (Cat. No. CT4-H52A4) with a linear range of 0.1-0.8 ng/mL (Routinely tested).

  • Background
    CTLA-4 (Cytotoxic T-Lymphocyte Antigen 4) is also known as CD152 (Cluster of differentiation 152), is a protein receptor that downregulates the immune system. CTLA4 is a member of the immunoglobulin superfamily, which is expressed on the surface of Helper T cells and transmits an inhibitory signal to T cells. The protein contains an extracellular V domain, a transmembrane domain, and a cytoplasmic tail. Alternate splice variants, encoding different isoforms. CTLA4 is similar to the T-cell co-stimulatory protein, CD28, and both molecules bind to CD80 and CD86, also called B7-1 and B7-2 respectively, on antigen-presenting cells. CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Intracellular CTLA4 is also found in regulatory T cells and may be important to their function. Fusion proteins of CTLA4 and antibodies (CTLA4-Ig) have been used in clinical trials for rheumatoid arthritis.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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CTLA-4 Products

  • Name:Cytotoxic T-Lymphocyte-Associated Antigen 4
  • Classification:Homo sapiens
  • Number of Launched Drugs:1 Details
  • Number of Drugs in Clinical Trials:22 Details
  • Lastest Research Phase:Approved
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