This protein carries a mouse IgG2a Fc tag at the C-terminus. The protein has a calculated MW of 40.6 kDa. The protein migrates as 50 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation and 100 kDa under non-reducing (NR) condition.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human CTLA-4, mouse IgG2a Fc tag, low endotoxin on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Human B7-1, Fc Tag (HPLC-verified) (Cat. No. B71-H5259) at 2 μg/mL (100 μL/well) can bind Human CTLA-4, mouse IgG2a Fc tag, low endotoxin (Cat. No. CT4-H52A4) with a linear range of 0.06-1 ng/mL (QC tested).
Loaded Human CTLA-4, mouse IgG2a Fc tag, low endotoxin (Cat. No. CT4-H52A4) on Protein A Biosensor, can bind Human B7-1, His Tag (Cat. No. B71-H5228) with an affinity constant of 0.52 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Loaded Human CTLA-4, mouse IgG2a Fc tag, low endotoxin (Cat. No. CT4-H52A4) on Protein A Biosensor, can bind Human B7-2, His Tag (Cat. No. CD6-H5223) with an affinity constant of 1.6 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Authors: Zhao Y, et al
Journal: Immunity 2019
Application: Cell Culture
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