This protein carries a mouse IgG2a Fc tag at the C-terminus. The protein has a calculated MW of 40.6 kDa. The protein migrates as 50 kDa on a SDS-PAGE gel under reducing (R) condition due to glycosylation and 100 kDa under non-reducing (NR) condition.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Human CTLA-4, mouse IgG2a Fc tag, low endotoxin on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Human B7-1, Fc Tag (Cat. No. B71-H5259) at 2 μg/mL (100 μL/well) can bind Human CTLA-4, mouse IgG2a Fc tag, low endotoxin (Cat. No. CT4-H52A4) with a linear range of 0.1-2 ng/mL (QC tested).
Authors: Zhao Y, et al
Journal: Immunity 2019
Application: Cell Culture
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High quality SARS-CoV-2 S protein, S1 protein and human ACE2 protein based on the human cell expression platform of HEK293.
ACROBiosystems has developed SARS-CoV-2 antigens specifically designed and optimized for serological test kits which are customized formulation available, capable of supplying in bulk order and functionality confirmed.
The affinity constant of SARS-CoV-2 S protein (active trimer) binding to human ACE2 protein was 35.3 nM as verified by SPR.
Binding signal of biotinylated human ACE2 and SARS-CoV-2 S protein RBD was inhibited by SARS-CoV-2 inhibitor with the IC50 10.69 nM.
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