This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 77.0 kDa. The protein migrates as 90-116 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
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Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Human Siglec-2, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Human Siglec-2, His Tag (Cat. No. CD2-H52H8) was more than 90% and the molecular weight of this protein is around 100-125 kDa verified by SEC-MALS.
Immobilized Human Siglec-2, His Tag (Cat. No. CD2-H52H8) at 2 μg/mL (100 μL/well) can bind Anti-Human CD22 MAb with a linear range of 0.3-5 ng/mL (QC tested).
Immobilized Human Siglec-2, His Tag (Cat. No. CD2-H52H8) at 2 μg/mL, add increasing concentrations of anti-CD22xCD19 scFv and then add Biotinylated Human CD19 (20-291), Fc,Avitag (Cat. No. CD9-H82F7) at 0.2 μg/mL. Detection was performed using HRP-conjugated streptavidin with sensitivity of 3.9 ng/mL (Routinely tested).
Loaded Anti-Human CD22 MAb (human IgG1) on AHC Biosensor, can bind Human Siglec-2, His Tag (Cat. No. CD2-H52H8) with an affinity constant of 133 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
293 cells were transfected with anti-CD22-scFv and RFP tag. 2e5 of the cells were first stained with B. Human Siglec-2, His Tag (Cat. No. CD2-H52H8, 3 μg/mL) and C. His Tag Protein Control, followed by FITC Anti-6xHis Tag Antibody. A. Non-transfected 293 cells and C. His Tag Protein Control were used as negative control. RFP was used to evaluate CAR (anti-CD22-scFv) expression and FITC was used to evaluate the binding activity of Human Siglec-2, His Tag (Cat. No. CD2-H52H8).
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