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Human BACE-1 Protein, Tag Free (active enzyme, MALS verified)

COADS MS
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Cat. No. / Size
Price
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BA1-H5213-100ug
$290.00
BA1-H5213-1mg (200ug X 5)
$2040.00
ETA of in-stock products:2 business days
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Product Details

  • Synonyms

    BACE1, ASP2, BACE, FLJ90568, HSPC104, KIAA1149, Memapsin-2, Beta-Secretase, β-secretase

  • Source

    Human BACE-1, Tag Free (BA1-H5213) is expressed from human 293 cells (HEK293). It contains AA Thr 22 - Thr 457 (Accession # NP_036236.1).

    Predicted N-terminus: Thr 22

    Request for sequence

  • Molecular Characterization

    BACE-1 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries no "tag".

    The protein has a calculated MW of 49.0 kDa. The protein migrates as 53-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >95% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 50 mM Tris, pH8.0 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Shipping and Storage

    This product is shipped at ambient temperature.

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.

  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    BACE-1 SDS-PAGE

    Human BACE-1, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

  • SEC-MALS

    BACE-1 SEC-MALS

    The purity of Human BACE-1, Tag Free (Cat. No. BA1-H5213) is more than 95% and the molecular weight of this protein is around 55-65 kDa verified by SEC-MALS.

    Report

  • Bioactivity

    Measured by its ability to cleave a fluorogenic peptide substrate, Mca-SEVNLDAEFRK(Dpn)RR-NH2. The specific activity is >3.5 pmol/min/µg, as measured under the described conditions (QC tested).

    Protocol

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Background

Beta-secretase 1 (BACE1) is also known as beta-site APP cleaving enzyme 1 (beta-site amyloid precursor protein cleaving enzyme 1), memapsin-2 (membrane-associated aspartic protease 2), and aspartyl protease 2 (ASP2), β-Secretase , and is a member of the peptidase A1 protein family, BACE1 is a type I integral membrane glycoprotein and aspartic protease that is found mainly in the Golgi. BACE1 is an aspartic-acid protease important in the pathogenesis of Alzheimer's disease, and in the formation of myelin sheaths in peripheral nerve cells. The transmembrane protein contains two active site aspartate residues in its extracellular protein domain and may function as a dimer. This protease is responsible for the proteolytic processing of the amyloid precursor protein (APP). Generation of the 40 or 42 amino acid-long amyloid-β peptides that aggregate in the brain of Alzheimer's patients requires two sequential cleavages of the APP. Extracellular cleavage of APP by BACE creates a soluble extracellular fragment and a cell membrane-bound fragment referred to as C99. The elevation of BACE1 levels can be induced by amyloid plaques surrounding neurons at early stages of pathology before neuron death occurs, and may drive a positive-feedback loop in AD.

Recent Advances

 
Drug Development Progress
  • English Name：

    Beta-secretase 1

  • Category：

  • Approved Drugs：

    0 Details

  • Drugs in Clinical Trials：

    3 Details

  • Highest Development Stage：

    Phase 3 Clinical

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