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Your Position: Home > Protein > Alpha-Synuclein > ALN-H52H8

Human Alpha-Synuclein Protein, His Tag

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  • Synonym
    SNCA,NACP,PARK1,alpha-Synuclein
  • Source
    Human Alpha-Synuclein, His Tag(ALN-H52H8) is expressed from E. coli cells. It contains AA Met 1 - Ala 140 (Accession # P37840-1 ).
    Predicted N-terminus: Met 1
  • Molecular Characterization
    Alpha-Synuclein Structure

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 16.3 kDa. The protein migrates as 19 kDa under reducing (R) condition (SDS-PAGE).

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Sterility
    The sterility testing was performed by membrane filtration method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 100 mM NaAC, pH7.0 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Alpha-Synuclein SDS-PAGE

Human Alpha-Synuclein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ThT Assay
 Alpha-Synuclein THT

Thioflavin T is a sensitive fluorescence reporter of fibrils formulation that binds to beta sheet-rich structures. The alpha synuclein pre-formed fibrils (Cat. No. ALN-H51H4) is able to induce the aggregation of alpha synuclein monomers (Cat. No. ALN-H52H8) (Routinely tested).

  • Background
    Neuronal protein that plays several roles in synaptic activity such as regulation of synaptic vesicle trafficking and subsequent neurotransmitter release. Participates as a monomer in synaptic vesicle exocytosis by enhancing vesicle priming, fusion and dilation of exocytotic fusion pores. Mechanistically, acts by increasing local Ca2+ release from microdomains which is essential for the enhancement of ATP-induced exocytosis. Acts also as a molecular chaperone in its multimeric membrane-bound state, assisting in the folding of synaptic fusion components called SREs (Soluble NSF Attachment Protein REceptors) at presynaptic plasma membrane in conjunction with cysteine string protein-alpha/DJC5. This chaperone activity is important to sustain normal SRE-complex assembly during aging. Plays also a role in the regulation of the dopamine neurotransmission by associating with the dopamine transporter (DAT1) and thereby modulating its activity.
  • Clinical and Translational Updates

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Price(USD) : $160.00

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  • Number of Launched Drugs:0 Details
  • Number of Drugs in Clinical Trials:20 Details
  • Latest Research Phase:Phase 3 Clinical

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