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Your Position: Home > Viral Proteins > S protein RBD > SPD-M52H6

MERS S protein RBD, His Tag

  • Synonym
    Spike,S protein RBD,Spike glycoprotein Receptor-binding domain,S glycoprotein RBD,Spike protein RBD,COVID-19
  • Source
    MERS S protein RBD, His Tag (SPD-M52H6) is expressed from human 293 cells (HEK293). It contains AA Glu 367 - Tyr 606 (Accession # K0BRG7-1).
    Predicted N-terminus: Glu 367
  • Molecular Characterization
    Online(Glu 367 - Tyr 606) K0BRG7-1

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 28.2 kDa. The protein migrates as 33-40 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 . Normally Trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
MERS S protein RBD, His Tag (Cat. No. SPD-M52H6) SDS-PAGE gel

MERS S protein RBD, His Tag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

Bioactivity-ELISA
MERS S protein RBD, His TagMERS S protein RBD, His Tag (Cat. No. SPD-M52H6) ELISA bioactivity

Immobilized Human DPPIV, Fc Tag (Cat. No. DP4-H5266) at 5 μg/mL (100 μL/well) can bind MERS S protein RBD, His Tag (Cat. No. SPD-M52H6) with a linear range of 0.6-5 ng/mL (QC tested).

MERS S protein RBD, His TagMERS S protein RBD, His Tag (Cat. No. SPD-M52H6) ELISA bioactivity

Immobilized MERS S protein RBD, His Tag (Cat. No. SPD-M52H6) at 5 μg/mL (100 μL/well) can bind Monoclonal Anti-SARS-CoV-S protein RBD Antibody, Human IgG1 with a linear range of 0.313-2.5 μg/mL (Routinely tested).

  • Background
    It's been reported that SARS-CoV-2 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
  • References
    • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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    Price(USD) : $400.00

    Price(USD) : $2600.00

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