Human CXCL4 / PF4 Protein, His Tag

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Cat. No. / Size
Price
Qty
CX4-H52H9-100ug
$430.00
CX4-H52H9-1mg (250ug X 4)
$2720.00
ETA of in-stock products:2 business days

Product Details

  • Synonyms

    PF4, CXCL4, MGC138298, SCYB4, C-X-C motif chemokine 4, Iroplact, Oncostatin-A

  • Source

    Human CXCL4, His Tag (CX4-H52H9) is expressed from human 293 cells (HEK293). It contains AA Glu 32 - Ser 101 (Accession # P02776-1).

    Predicted N-terminus: Glu 32

    Request for sequence
  • Molecular Characterization

    CXCL4 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 9.7 kDa. The protein migrates as 13 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 10 mM Sodium Citrate, 150 mM NaCl, pH5.0 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    CXCL4 SDS-PAGE

    Human CXCL4, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

  • Bioactivity-ELISA

     CXCL4 ELISA

    Immobilized Human CXCL4, His Tag (Cat. No. CX4-H52H9) at 5 μg/mL (100 μL/well) can bind Human CCL5, Fc Tag (Cat. No. CC5-H5251) with a linear range of 0.078-1.25 μg/mL (QC tested).

    Protocol

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Background

CXCL4, or Platelet factor 4 (PF4), is a small cytokine belonging to the CXC chemokine family. This chemokine is released from the alpha granules of activated platelets in the form of a homotetramer which has high affinity for heparin and is involved in platelet aggregation. CXCL4 is chemotactic for neutrophils and monocytes and also functions as an inhibitor of hematopoiesis, angiogenesis and T-cell function. CXCL4/PF4 is up-regulated in human liver fibrosis and that it plays a nonredundant, functional role in experimental liver fibrosis by mediating stellate cell proliferation, migration, and intrahepatic immune cell recruitment.

Recent Advances

 
Drug Development Progress
  • English Name:

    Platelet factor 4

  • Category:

  • Approved Drugs:

    0 Details

  • Drugs in Clinical Trials:

    0 Details

  • Highest Development Stage:

    Preclinical

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