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We offer IHC Antibody Products against multiple biomarker targets with sufficient validation, reliable quality, flexible authorization and service support!
Cynomolgus LRRC32&TGFB1 Heterotrimer protein, His Tag&Tag Free (the molar ratio of LRRC32 & TGF-beta 1 equals 1:2) is produced by co-expression of LRRC32 and TGF-beta 1, which has a calculated MW of 68.1 kDa (LRRC32), 28.5 (LAP) and 12.8 kDa (mature TGF-beta 1) respectively. LRRC32 is fused with a polyhistidine tag at the C-terminus and TGF-beta 1 contains no tag. The reducing (R) Heterotrimer protein migrates as 70 kDa (LRRC32), 37-43 kDa (LAP) and 13 kDa (mature TGF-beta 1) due to glycosylation respectively.
>90% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Cynomolgus LRRC32&TGF-beta 1 Heterotrimer Protein, His Tag&Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90%.
Immobilized Cynomolgus LRRC32&TGF-beta 1 Heterotrimer Protein, His Tag&Tag Free (Cat. No. GA1-C52W7) at 1 μg/mL (100 μL/well) can bind Biotinylated Human ITGAV&ITGB6 Heterodimer Protein, His,Avitag&Tag Free (Cat. No. IT6-H82E4) with a linear range of 1-39 ng/mL (QC tested).
Anti-LRRC32 antibody captured on Protein A Chip can bind Cynomolgus LRRC32&TGF-beta 1 Heterotrimer Protein, His Tag&Tag Free (Cat. No. GA1-C52W7) with an affinity constant of 51.9 pM as determined in SPR assay (Biacore 8K) (Routinely tested).
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