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Use the kit after you extract residual DNA from test samples. For achieving the better DNA recovery, it is recommended to use the resDetect™ resDNA Sample Preparation Kit (Magnetic Beads) (Cat. No. OPA-R005) in combination.
The kit is used for quantitative detection of residual Plasmid DNA in biopharmaceutical production.
For use in quality control/manufacturing process only.
It is for research use only.
Figure 1 High sensitivity and broad dynamic range using the Plasmid resDNA Quantitation Kit. (A) Typical analysis results of obtained with Linearize DNA control Standard 1 (4×10⁶ copies/µL) to 6 (4×10 copies/µL). (B) The standard curve of the Linearize DNA control 10-fold dilution series. PCR efficiency should be 90-110%. (C) Typical analysis results of obtained with Circular DNA control Standard 1 (2×10⁶ copies/µL) to 5 (2×10² copies/µL). (D) The standard curve of the Circular DNA control 10-fold dilution series. PCR efficiency should be 90-110%.
To evaluate the specificity of assay using Plasmid resDNA Quantitation Kit, no template samples spiked with unrelated DNA were tested. Results shown in the following Table. Unrelated DNA (HEK293, CHO, Vero, Pichia pastoris, and MDCK) were detected in assay, however the values of Mean were outside the range of standard curve (4×106 copies/µL to 4×10 copies/µL).
To evaluate the recovery of assay using Plasmid resDNA Quantitation Kit, three different concentrations of DNA samples were tested. Results shown in the following Table. All samples had recoveries between 80%-110%.
| ID | Components | Size |
| OPA-R009-01 | Plasmid Primer & Probe Mix | 550 μL |
| OPA-R009-02 | 2×qPCR Master Mix | 1.6 mL |
| OPA-R009-03 | Circular DNA Control 2×10⁸ copies/μL | 50 μL |
| OPA-R009-04 | Linearize DNA Control 4×10⁸ copies/μL | 50 μL |
| OPA-R009-05 | DNA Dilution Buffer | 1.5 mL×3 |
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