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Human beta-Galactosidase-1 Protein, His Tag (active enzyme)

COADS MS
DS

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Cat. No. / Size
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BG1-H52H3-100ug
$360.00
BG1-H52H3-1mg
$2575.00
ETA of in-stock products:2 business days
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Product Details

  • Synonym

    EBP, ELNR1, MPS4B

  • Source

    Human beta-Galactosidase-1 Protein, His Tag (BG1-H52H3) is expressed from human 293 cells (HEK293). It contains AA Leu 24 - Val 677 (Accession # P16278-1).

    Predicted N-terminus: Leu 24

    Request for sequence

  • Molecular Characterization

    beta-Galactosidase-1 Structure

    Other Tags and Version Biotin & Other Labeled Version

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 75.5 kDa. The protein migrates as 80-95 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin

    Less than 1.0 EU per μg by the LAL method / rFC method.

  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Supplied as 0.2 μm filtered solution in 50 mM Tris, 150 mM NaCl, 20% Glycerol, pH7.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Shipping

    This product is supplied and shipped with dry ice, please inquire the shipping cost.

  • Storage

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -70°C or lower upon receipt;
    2. -70°C for 3 months under sterile conditions.

  • ACRO Quality Management System

    1. QMS(ISO, GMP)
    2. Quality Advantages
    3. Quality Control Process

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Performance Data

  • SDS-PAGE

    beta-Galactosidase-1 SDS-PAGE

    Human beta-Galactosidase-1 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

  • Bioactivity

    Measured by its ability to cleave a fluorogenic substrate, 4-Methylumbelliferyl-β-D-galactopyranoside. The specific activity is >1,800 pmol/min/μg, as measured under the described conditions (QC tested).

    Protocol

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Background

This gene encodes a member of the glycosyl hydrolase 35 family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature lysosomal enzyme. This enzyme catalyzes the hydrolysis of a terminal beta-linked galactose residue from ganglioside substrates and other glycoconjugates. Mutations in this gene may result in GM1-gangliosidosis and Morquio B syndrome. [provided by RefSeq, Nov 2015]

Recent Advances

 
Drug Development Progress
  • English Name：

    Beta-galactosidase

  • Category：

  • Approved Drugs：

    0 Details

  • Drugs in Clinical Trials：

    1 Details

  • Highest Development Stage：

    Phase 2 Clinical

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