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Your Position: Home > Protein > CD73 > CD3-S52H3

Sus scrofa CD73 / NT5E Protein, His Tag (active enzyme, MALS verified)

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  • Synonym
    CD73,NT5E,5'-Nucleotidase,5'-NT,NT5,NTE
  • Source
    Sus scrofa CD73, His Tag (CD3-S52H3) is expressed from human 293 cells (HEK293). It contains AA Trp 27 - Ser 549 (Accession # XP_001927130.1).
    Predicted N-terminus: Trp 27
  • Molecular Characterization
    Online(Trp 27 - Ser 549) XP_001927130.1

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 59.5 kDa. The protein migrates as 60-65 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 20 mM Tris, 120 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Sus scrofa CD73, His Tag (Cat. No. CD3-S52H3) SDS-PAGE gel

Sus scrofa CD73, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-MALS
Sus scrofa CD73, His Tag (Cat. No. ) MALS images

The purity of Sus scrofa CD73, His Tag (Cat. No. CD3-S52H3) is more than 90% and the molecular weight of this protein is around 112-136 kDa verified by SEC-MALS.

Bioactivity

Measured by its ability to hydrolyze the 5’-phosphate group from the substrate adenosine-5’-monophosphate (AMP). The specific activity is > 50000 pmol/min/μg (QC tested).

  • Background
    5'-nucleotidase (5'-NT), also known as ecto-5'-nucleotidase or CD73 (cluster of differentiation 73), is an enzyme that is encoded by the NT5E gene. CD73 commonly serves to convert AMP to adenosine. Ecto-5-prime-nucleotidase (5-prime-ribonucleotide phosphohydrolase) catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from ecto-NT5 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate. Rare allelic variants are associated with a syndrome of adult-onset calcification of joints and arteries (CALJA) affecting the iliac, femoral, and tibial arteries reducing circulation in the legs and the joints of the hands and feet causing pain.
  • Clinical and Translational Updates
      
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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