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Use the kit after you extract host cell DNA from test samples. To achieve the best performances, resDNA Sample Preparation Kit (Magnetic Beads) (Cat. No. OPA-R005) is commanded for DNA extraction.
The kit is used for quantitative detection of residual E1A&SV40LTA DNA in biopharmaceutical production.
For use in quality control/manufacturing process only.
It is for research use only.
Figure 1. High sensitivity and broad dynamic range using the E1A&SV40LTA resDNA Quantitative Kit. (A) Typical analysis results of obtained with E1A DNA Control Standard 1 (4×10⁶ copies/µL) to 6 (4×10 copies/µL). (B) The standard curve of the E1A DNA Control 10-fold dilution series. PCR efficiency should be 90-110%. (C) Typical analysis results of obtained with SV40LTA DNA Control Standard 1 (4×10⁶ copies/µL) to 6 (4×10 copies/µL). (D) The standard curve of the SV40LTA DNA control 10-fold dilution series. PCR efficiency should be 90-110%.
To evaluate the specificity of assay using E1A&SV40LTA resDNA Quantitation Kit, no template samples spiked with unrelated DNA were tested. Results shown in the following Table. Unrelated DNA (CHO, Vero, Pichia yeast) were not detected in assay, and the values of E. coli and MDCK were outside the range of standard curve (4×10⁶ copies/µL to 4×10 copies/µL).
To evaluate the recovery of assay using E1A&SV40LTA resDNA Quantitation Kit, three different concentrations of DNA samples were tested. Results shown in the following Table. All samples had recoveries between 78%-100%.
| ID | Components | Size |
| OPA-R007-01 | E1A&SV40LTA Primer & Probe Mix | 550 μL |
| OPA-R007-02 | 2×qPCR Master Mix | 1.6 mL |
| OPA-R007-03 | Linearize DNA Control 4×10⁸ copies/μL | 50 μL |
| OPA-R007-04 | DNA Dilution Buffer | 1.5 mL×3 |
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