Antibody-Drug Conjugates (ADCs): the Magic Bullets for Treatment

Home
Products

products by category

Recombinant proteins
GMP Grade CytokinesNew Fc receptor proteins FcRn Proteins IgG Fc Proteins (Isotype Control) CGT development solutions New Targets of CAR-T cell therapy New Generation Tools for CAR DetectionStar Staining FDA DMF filed proteins Multi-pass TpsHot Immune checkpoint proteins ADC targets BsAb targets TME pathway targets CytokinesHot Enzymes Llama IgG Fc fusion protein Biosimilar drug targets Biomarkers & CD antigens All recombinant proteins

Antibodies
Anti-idiotypic antibody Anti-FMC63 mAb Neutralizing antibodies IgG Isotype Controls: Anti-DNP Antibodies
Kits/Beads
Pre-coupling magnetic beads Inhinitor screening kits ELISA Assay Kits for Quantitative analysis of therapeutic antibodies in serum
Support on research about infectious diseases Hot
Core reagents for infectious disease researchNew SARS-CoV-2 related products SARS-CoV-2 mutant products SARS-CoV-2 related kits Reagents for vaccine development Reagents for diagnostic development

Featured products

Pre-labeled proteins
New Generation Tools for CAR DetectionStar Staining Biotinylated protein collection FITC & PE labeled proteins
Full-length multi-pass TpsHot
Multi-pass Tps Claudin 18.2 GPCRs CD20 proteins
Proteins for Neuroscience
Proteins for Neuroscience New
Cytokines
ActiveMax Cytokines Hot Promising Therapeutic Targets Hot
Streptavidin (SA) series Products
SA, SA Coated Plates, SA Magnetic Beads New
Isotype Controls
Anti-DNP AntibodiesHot IgG Fc ProteinsHot Serum AlbuminHot

Hot targets
CD19 BCMA CD3 proteins IL-2 and IL-2 receptor proteins IL-6 and IL-6 receptor proteins Claudin 18.2 GPCRs CD20 proteins CD39/CD73 proteins B7 families TNFSF proteins VEGF proteins PD1 proteins TIGIT Siglec-15 proteins Integrin CD24&siglec-10

Brands
FLAG Star Staining ViruStop Aneuro ComboX ActiveMax GENPower
Services

Innovation Lab
SPR /BLI analytical serviceNew Enzyme-Linked Immunosorbent Assay (ELISA) Service New Meso Scale Discovery (MSD) ServiceNew Anti-Idiotypic Antibody Development ServiceNew Pseudovirus Neutralization Assay ServiceNew MHC Class I Complex Custom ServiceNew

Licensing Opportunities
Target 4-1BB monoclonal antibody New PD-1/CD80 bispecific antibodyNew Antagonizing TSP1/CD36 Polypeptide Blocking the activation of TGF-β1New Anti-PD-L1 / TIGIT bispecific antibodyNew TIGIT-Fc proteinNew Novel small molecule drug for cancer cachexiaNew
Supports

How to Order Shipping & Payments Global Distributors Testimonials FAQs Download COA
Applications

The Process of How SARS-CoV-2 Infects the Host Quantitative Analysis of mAb in Serum Evaluation of CAR Expression Claudin18.2-VLP：designed for CAR detection SPR & BLI：Binding activity assay SEC-MALS: Aggregation state and purity
News

News Insights Promotions Exhibitions
Resources

Download Brochures Download E-resources Download COA
ACROBiosystems

About Us Join Us Contact Us

Background

In recent years, Antibody-Drug Conjugates (ADCs) may serve as a novel therapeutic modality for many cancer patients. Indeed, three key elements define an ADC: the antibody directed against a specific tumor antigen, the cytotoxin/payload and the cleavable/uncleavable linker connecting the payload to the antibody ^[1]. Specific target recognition and effective toxin make ADCs veritable "magic bullet". These 'magic bullets' deliver treatment that is both highly targeted and highly potent, seeking out and attacking cancerous cells throughout the body, while avoiding harm to healthy cells. Reasonable selection of targets, antibody, linker, payload, and their rational combinations are crucial for an effective treatment. Among these elements, target selection, linker selection and evaluating preclinical /clinical efficacy are considered critical factors for ADCs development. >>> Download the ebook: Key point of ADCs design

To meet the needs of ADCs development, ACROBiosystems can provide:

A variety of high-quality target proteins;
SPR/BLI analytical service;
MMPs/Cathepsin/uPA for cleavable linker;
Anti-payload antibodies, anti-idiotypic antibodies(ADA) for immunogenicity and PK analysis.

All products and services can meet the entire process of ADCs from antibody preparation, initial screening, production to quality control, facilitating your ADCs development.

Hot Products

High-quality ADC target proteins

Features

>> 50+ ADC targets, such as Trop-2, Nectin-4,LIV-1 and ROR1;
>> Various species & tag;
>> High purity verified by MALS;
>> High bioactivity verified by ELISA / SPR / BLI / FACS, etc.

Applications

Suitable for immunization / antibody screening / cross-reactivity/cell-based assay/QC.

Type	Molecule	Cat. No.	Product Description	Preorder/Order
Cathepsin	Cathepsin B	CTB-H5222	Human Cathepsin B / CTSB Protein, His Tag (active enzyme)	Order
PLAU	PLAU	PLU-H5229	Human PLAU / uPA Protein, His Tag	Order
PLAU	PLU-H5228	Human PLAU / uPA Protein, His Tag (activated by trypsin) (active enzyme)	Order
MMPs	MMP-1	MM1-H5222	Human MMP-1 Protein, His Tag	Order
MMP-2	MM2-H5225	Human MMP-2 Protein, His Tag	Order
MMP-2	MM2-H82E3	Biotinylated Human MMP-2 Protein, His,Avitag™	Preorder
MMP-2	MM2-M52H3	Mouse MMP-2 Protein, His Tag	Preorder
MMP-9	MM9-H5221	Human MMP-9 Protein, His Tag (active enzyme)	Order

Cat.No.	Product description	Neutralizing activity	Affinity K_D, nM	Application
MME-M5252^NEW	Anti-MMAE Antibodies	/	/	PK assay; ELISA
ADB-Y19	Anti-Adalim*mab Antibodies	Neutralizing Antibody	0.0013	ADA assay; Neutralizing assay; Indirect ELISA
ADB-Y23b	Anti-Adalim*mab Antibodies	Non-Neutralizing Antibody	/	PK bridging ELISA with ADB-BY17; Indirect ELISA
ADB-BY17	Biotinylated Anti-Adalim*mab Antibodies	Neutralizing Antibody	/	PK bridging ELISA with ADB-Y23b
BEB-Y9	Anti-Bevaciz*mab Antibodies	Neutralizing Antibody	1.92	ADA assay; Indirect ELISA
BEB-Y10	Anti-Bevaciz*mab Antibodies	Neutralizing Antibody	/	PK bridging ELISA with BEB-BY13; Neutralizing assay; Indirect ELISA
BEB-BY13	Biotinylated Anti-Bevaciz*mab Antibodies	Neutralizing Antibody	/	PK bridging ELISA with BEB-Y10
CEB-Y27	Anti-Cetux*mab Antibodies	Neutralizing Antibody	0.007	ADA assay; Neutralizing assay; Indirect ELISA
CEB-Y29	Anti-Cetux*mab Antibodies	Neutralizing Antibody	/	PK bridging ELISA with CEB-BY31; Neutralizing assay; Indirect ELISA
CEB-BY31	Biotinylated Anti-Cetux*mab Antibodies	Non-Neutralizing Antibody	/	PK bridging ELISA with CEB-Y29
RIB-Y35c	Anti-Ritux*mab Antibodies	Neutralizing Antibody	/	ADA assay; Neutralizing assay; Indirect ELISA
TRB-Y1b	Anti-Trastuz*mab Antibodies	Neutralizing Antibody	/	PK bridging ELISA with TRB-Y5b; Neutralizing assay; Indirect ELISA
TRB-Y5b	Anti-Trastuz*mab Antibodies	Non-Neutralizing Antibody	/	PK bridging ELISA with TRB-Y1b

Case study

High purity verified by MALS

The purity of Human Her2, His Tag (Cat. No. HE2-H5225) was more than 90% and the molecular weight of this protein is around 80-95 kDa verified by SEC-MALS.

High affinity validated by ELISA

HER2 protein's affinity verified by ELISA

Immobilized Human TROP-2, His Tag (Cat. No. HE2-H5225) at 1 μg/mL (100 μL/well) can bind Mouse Monoclonal Antibody Against Human TROP-2, Mouse IgG1 with a linear range of 0.1-2 ng/mL (QC tested).

Protocol

PSMA protein's affinity verified by ELISA

Immobilized Human PSMA, His Tag (Cat. No. PSA-H52H3) at 2 μg/mL (100 μL/well) can bind Monoclonal Anti-Human PSMA Antibody, Human IgG1 with a linear range of 0.3-39 ng/mL (Routinely tested).

Protocol

High affinity validated by SPR

LIV-1 protein's affinity verified by SPR

Anti-LIV-1 mAb captured on CM5 chip via anti-human IgG Fc antibody can bind Human LIV-1, His Tag (Cat. No. LV1-H5223) with an affinity constant of 5.24 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

ProtocolClick to consult SPR service

Trastuz*mab captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind Human Her2, His Tag (Cat. No. HE2-H5225) with an affinity constant of 1.07 nM as determined in a SPR assay.

ProtocolClick to consult SPR service

High affinity validated by BLI

Nectin-4 protein's affinity verified by BLI

Loaded Mouse Nectin-4, His Tag (Cat. No. NE4-M52H3) on HIS1K Biosensor, can bind Human Nectin-1, Fc Tag (Cat. No. PV1-H5253) with an affinity constant of 0.12 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

ProtocolClick to consult BLI service

High affinity validated by FACS

2e5 of anti-ROR1 CAR-293 cells were stained with 100 μL of 10 μg/mL of Human / Cynomolgus / Rhesus macaque ROR1 (39-151, Ig-like domain), His Tag (Cat. No. RO1-H5221) and negative control protein respectively, washed and then followed by PE-anti-His Tag and analyzed with FACS (Routinely tested).

Protocol

Proteases for peptide linker

In the design and development of ADC, linker affects the stability of ADCs in systematic circulation, the speed of lysosomal processing, etc. There are two main categories of ADC linkers in current ADC drugs, cleavable linkers, and non-cleavable linkers.

Peptide linker as a protease-sensitive cleavable linker, is used in most ADCs that are undergoing clinical testing. In the design of peptide linker, it is necessary to consider the selection of a suitable protease for linker cleavage, such as: MMPs/Cathepsin/ PLAU ^[2].

Thus, ACROBiosystems has developed a series of MMPs, Cathepsin and uPA protein products for cleavage of Linker.

More anti-payload antibodies (Anti-Dxd antibody/Anti-SN-38 antibody/Anti-SN-38 antibody) are coming soon！

Case study

Products	Cat.No：CTB-H5222 Human Cathepsin B / CTSB Protein, His Tag (active enzyme)	Cat.No：MM9-H5221 Human MMP-9 Protein, His Tag (active enzyme)
Hydrolyzed substrate	Fluorogenic peptide substrate Z-LR-AMC	Fluorogenic peptide substrate Mca-PLGL-Dpa-AR-NH2
Enzymatic activity （pmol/min/μg）	> 2500	> 2500

PK analysis

Supporting Immunogenicity and PK analysis, ACRO provides series of anti-MMAE antibodies and anti-idiotypic antibodies with high affinity, high specificity and high sensitivity.

Used for the positive reference of ADCs preclinical/clinical immunogenicity and PK analysis;
Save customer's time to prepare antibodies;
Complete methodology protocols are available freely.

Case study

PK assay: Anti-MMAE antibody binding MMAE with high specificity

The EC50 value of anti-MMAE antibody to MMAE-conjugated hRS7 (MMAE-ADC) was 0.12 μg/ml. The unconjugated hRS7 antibody was used as negative control

Protocol

Anti-MMAE antibody binding MMAE-ADC with high specificity

Immobilized Disitamab Vedotin (RC48) at 0.2 μg/mL (100 μL/well) can bind Mouse Anti-MMAE Antibody, Mouse IgG1 (Cat. No. MME-M5252) with a linear range of 0.1-2 ng/mL (QC tested).

Protocol

PK assay: Specific detection of antibody drug levels in the body

PK assay for ADCs: Specific detection of antibody drug levels in the body

Detection of Ritux*mab by antigen-capture ELISA (0.1% serum).

Detection of Ritux*mab by anti-idiotypic capture ELISA (0.1% serum).

Detection of Ritux*mab by anti-idiotypic bridging ELISA (10% serum).

Method	Coated	Sample	Linear range (µg/mL)	Sensitivity (µg/mL)	Advantage
Antigen capture ELISA	CD20	Goat anti-human IgG	—	—	Simple and universal method
Anti-idiotypic capture ELISA	Anti-Ritux*mab Antibodies	Goat anti-human IgG	0.156-10	0.156	It is a simple method for extracting CD20
Bridging ELISA by anti-idiotypic antibodies	Anti-Ritux*mab Antibodies	Biotinylated Anti-Ritux*mab Antibodies	0.012-0.78	0.012	CD20 can be obtained with good sensitivity and low background noise

Comparison between anti-idiotypic capture ELISA and anti-idiotypic bridging elisa for Ritux*mab detection in patient samples

SPR/BLI analytical service & Anti-idiotypic antibody development service

In addition, relying on a very capable and highly professional team, ACROBiosystems also provides customers with high-quality integrated SPR&BLI analytical service and one-stop services about monoclonal/polyclonal anti-idiotype antibody, facilitating your ADCs development.

SPR/BLI analytical service

Cooperated with hundreds of industrial and scientific research users;
Proteins offered for free;
High standard quality management control system;
Next day reports at the earliest;
Support more than 300 drugs until clinical applications.

Anti-idiotypic antibody development service

ACRO proteins supplied for free;
Quick response, one-to-one service from the project team, real-time follow-up;
Provide one-stop service from antigen preparation to detection kit development;
Guaranteed delivery of PK/ADA detection reagents whose sensitivity meets regulatory requirements.