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Anti-Idiotypic Antibody

Background

Anti-idiotypic antibody is an antibody that recognizes the variable region of another antibody thus producing a specific binding. Anti-idiotypic antibodies are widely used in drug development. They can be used as an important reference for immunogenicity analysis and can specifically detect antibody drug levels in the body. Anti-idiotypic antibody is, therefore, an important reagent for pharmacokinetics research.

As a global brand focusing on protein technology, products, and services in the development of biological drugs, ACROBiosystems is committed to providing high quality target antigens and key reagents and related services required in the development of targeted therapeutic drugs. ACRO provides series of anti-idiotypic antibodies with high affinity, high specificity, and high sensitivity. At the same time, in order to meet the diverse needs of customers, ACRO also provides one-stop services from antigen preparation to monoclonal anti-idiotype antibody, polyclonal anti-idiotype antibody, pharmacokinetic and immunogenicity test kit developments.

Service content
Anti-idiotypic antibody
ada_2
Antigen blocking type(Neutralizing)
Paratope specific
Block antigen-antibody binding
Detection of free antibody drugs
Detection of neutralizing antibodies
ada_3
Antigen non-blocking type (Non-neutralizing)
Paratope non-specific
Not block antigen-antibody binding
Detection of total antibody drugs (free, semi-bound, bound)
ada_4
Drug target compound type
Specific binding drug target complex
Not block antigen- antibody binding
Specific detection of bound antibody drugs
NamePeriodDeliveryPrice

Anti-idiotypic rabbit polyclonal antibody preparation

10-14 weeks

  • 1. Pre-immunization serum;

  • 2. Rabbit antigen serum (can be prepared as freeze-dried powder)

  • 3. 1-10mg immunoaffinity chromatography purified antibody IgG, which can be freeze-dried (for long-term storage). If need to add preservatives and protein stabilizers, please inform us;

  • 4. 1-3mg of synthetic antigen;

  • 5. Complete experiment report;

One-on-one service of the project team, according to your needs, we will issue a customized  plan for you


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Anti-idiotypic mouse monoclonal antibody preparation

4-6 months

  • 1. Subclonal cell line;

  • 2. Ascites (freeze-dried powder);

  • 3. IgG: (immunopurified) (freeze-dried powder);

  • 4. IgG: (Protein G purification) (freeze-dried powder);

  • 5. Prepare report.

Development of a quantitative detection kit for blood drug concentration

6-8 weeks

  • 1. Methodology verification report;

  • 2. Instructions, COA;

  • 3. ELISA kit

Preparation of monoclonal anti-idiotypic antibodies

Monoclonal anti-idiotypic antibodies can be used in the detection of free type, bound type and total antibody drugs in pharmacokinetic studies. According to the antibody drug subtype, ACROBiosystems can provide anti-idiotypic antibodies of antigen-neutralizing and non-neutralizing subtypes.

Polyclonal anti-idiotypic antibody preparation service

Highly sensitive and diverse polyclonal anti-idiotypic antibodies can be used as a positive reference in immunogenicity studies, simulating the production of ADA inside the human body. ACROBiosystems can provide high-affinity and specific polyclonal anti-idiotypic antibody development services with short service cycles and low development costs.

Development of a quantitative detection kit for blood drug concentration

Pharmacokinetic (PK) is a subject that quantitatively studies the absorption, distribution, metabolism, and excretion of drugs in organisms. Since blood is the medium for the absorption, distribution, metabolism and excretion of any drugs and their metabolites in the body, the drug concentration in various body fluids and tissues have proportional relationship with the drug concentration in the blood. In order to facilitate the preclinical and clinical PK research of antibody drugs, it is particularly important to design an accurate experimental method to determine the blood drug concentration.

ACROBiosystems can customize and develop blood drug concentration detection kits for customers' diversified needs and applications while also providing key reagents for PK and immunogenicity analysis of biological drugs such as monoclonal antibodies, bispecific antibody, ADC, CAR-T, etc.

CAR-T
Promotion
Case study
PK-assay--Specific detection of antibody drug levels in the body
Testing methodCoatedSampleTesting
Antigen capture ELISACD20Antibodies to be testedGoat anti-human IgG
Anti-idiotypic capture ELISAAnti-Rituximab AntibodiesAntibodies to be testedGoat anti-human IgG
Bridging ELISA by anti-idiotypic antibodiesAnti-Rituximab AntibodiesAntibodies to be testedBiotinylated Anti-Rituximab Antibodies
Figure1. Detection of rituximab by antigen-capture ELISA (0.1% serum).
Figure2. Detection of rituximab by anti-idiotypic capture ELISA (0.1% serum).
Figure3. Detection of rituximab by anti-idiotypic bridging ELISA (10% serum).
Testing methodLinear range
(µg/mL)
Sensitivity
(µg/mL)
AdvantageDisadvantage
Antigen-capture ELISASimple method and good versatilityHigh background, no activity
Anti-idiotypic capture ELISA0.156-100.156Solve the difficulty in obtaining CD20, simple methodHigh background, only suitable for Rituxa biosimilar
Bridging ELISA by anti-idiotypic antibodies0.012-0.780.012Solve the difficulty in obtaining CD20, good sensitivity and low backgroundOnly applicable to Rituxan biosimilar
Advantage

Efficient

Fast response, one-on-one service of the project team, real-time follow-up of the experiment progress

Professional

Provide key reagents for ADA analysis for a variety of drugs (monoclonal antibody, bispecific antibody, ADC, CAR-T)

Multifunctional

Provide one-stop service from antigen preparation to monoclonal anti-idiotype antibodies, polyclonal anti-idiotype antibodies, PK and immunogenicity test kit developments

Reliable

Ensure delivery of PK/ADA test kits with sensitivity that meets regulatory requirements

Inquiry and order

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