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Your Position: Home > Star Staining Fluorescent-labeled Products New Generation Tools for CAR Detection

Star Staining Fluorescent-labeled Products
New Generation Tools for CAR Detection

Star Staining
INTRODUCTION
Star Staining fluorescent-labeled products are developed by a new-generation site-specific labeling technology with "Star Standard" quality at ACROBiosystems. These products are uniquely designed for detecting and monitoring CAR-T cells in clinical trials.
Fluorescent-labeled protein is a powerful tool for the detection of CAR expression in research and clinical samples by flow cytometry. These proteins are pre-labeled fluorescent dyes that can detect CAR expression by one-step staining with minimal background. However, the development of high-quality fluorescent-labeled proteins presents a major challenge and there are only few products commercially available so far.
Conjugation technique is a major bottleneck for developing high-quality fluorescent-labeled proteins. Currently, the most widely used labeling technique in the market is the traditional chemical labeling approach. It is a very easy method to covalently label protein with a fluorescent dye in a non-specific manner. However, random modification can lead to heterogeneous and may affect the bioactivity of the protein via blocking the protein-active site. Studies indicate that the new-generation site-specific labeling technology can attach fluorescent dyes to specific protein in site-specific manner. It provides a guarantee to the uniformity of the fluorescent-labeled products and avoids affecting protein activity. This property makes it a useful weapon for the development of high-quality fluorescent-labeled protein products with high specificity and sensitivity.
PRODUCT FEATURE
Traditional Chemical Labeling
Claudin18.2-VLP
Non-selective random labeling and affect natural structure
May cover the active sites to reduce bioactivity
Easy to aggregate due to change the hydrophilicity of proteins
High variation in batch-to-batch consistency
New-generation Site-specific Labeling
Nanodisc Platform
The labeling happens at the specific tag and far away from active sites of proteins
Maintain natural conformation and modification
Efficiently bio-orthogonal technology
High batch-to-batch consistency and uniformity.
STAR STAINING FEATURES

ACROBiosystems is striving for developing the high-standard detection reagents to support the development of CAR-T cell therapy. After years of research and experience by our technicians, we successfully established the "Star Staining" site-specific labeling platform and developed a series of exclusive fluorescent-labeled proteins with "Star Standard" quality. "Star Staining" products can be the powerful tools for detecting and monitoring CAR-T cells in clinical trials.

Using new-generation site-specific labeling technology to maintain natural bioactivity

High specificity and sensitivity verified by flow cytometry

Non-specific binding to non-transduced PBMCs

High batch-to-batch consistency and uniformity

PRODUCT LIST
MoleculeCat.No.Product descriptionPreorder/Order
BCMABCA-HF2H3FITC-Labeled Human BCMA / TNFRSF17 Protein, His Tag star staining

Order

CD19CD9-HF2H3FITC-Labeled Human CD19 (20-291) Protein, His Tag star staining

Order

MesothelinMSN-HF2H3FITC-Labeled Human Mesothelin / MSLN (296-580) Protein, His Tag star staining

Preorder

Siglec-2SI2-HF2H4FITC-Labeled Human Siglec-2 / CD22 Protein, His Tag star staining

Preorder

FMC63FM3-FY57P1FITC-Labeled Monoclonal Anti-FMC63 scFv Antibody, Mouse IgG1 (Y45) star staining

Preorder

DATA DISPLAY

High purity

High purity than 90% of Star Staining FITC-labeled Human BCMA
BCMA

FITC-Labeled Human BCMA, His Tag (Cat. No. BCA-HF2H3) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

BCMA

The purity of FITC-Labeled Human BCMA, His Tag (Cat. No. BCA-HF2H3) was more than 90% and the molecular weight of this protein is around 24-34 kDa verified by SEC-MALS.

High bioactivity

Higher binding activity than that of other competitors
FITC-labeled
FITC-labeled

Binding activity of FITC-Labeled Human BCMA and CD19 protein from two different vendors were evaluated by the FACS analysis. The result showed that ACRO's Star Staining FITC-Labeled Human BCMA (Cat. No. BCA-HF2H3) and CD19 (Cat. No. CD9-HF2H3) protein have a much higher binding activity than that of the other competitor.

Non-specific binding to non-transduced PBMCs

FITC-labeled

Non-specific binding to non-transduced PBMCs between FITC-Labeled Human BCMA Protein of Acro and competitor. 5e5 of non-transduced PBMCs were stained with FITC-Labeled Human BCMA Protein and anti-CD3 antibody, washed and then analyzed with FACS. PE signal was used to evaluate the expression of CD3+ T cells in non-transduced PBMCs, and FITC signal was used to evaluate the non-specific binding activity to non-transduced PBMCs.

Maintain natural bioactivity

High binding capacity before and after conjugation, verified by FACS and SPR
FITC-labeled

Binding activity of the Human BCMA before and after FITC labeling was evaluated in the above FACS analysis. The result shows that FITC-Labeled BCMA (Cat. No. BCA-HF2H3) and unconjugated Human BCMA have almost the same level of binding activity.

FITC-labeled
FITC-labeled

Binding affinity of the Human BCMA before and after FITC labeling was evaluated in the above SPR analysis (Biacore T200). The result shows that FITC-Labeled (Cat. No. BCA-HF2H3) and unconjugated Human BCMA, His Tag have almost the same level of affinity.

High batch-to-batch consistency

Binding activity of different lots of FITC-labeled Human BCMA protein was verified by FACS
FITC-labeled
FITC-labeled

Binding activity of two different size(left) or three different lots(right) of FITC-Labeled Human BCMA (Cat. No. BCA-HF2H3) against anti-BCMA CAR-293 cells was evaluated by flow cytometry. The result shows very high batch-to-batch consistency.

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REFERENCES

[1] Abbasov ME, Kavanagh ME, Ichu TA, et al. A proteome-wide atlas of lysine-reactive chemistry [published online ahead of print, 2021 Sep 9]. Nat Chem. 2021;10.1038/s41557-021-00765-4.

[2] Xu L, Kuan SL, Weil T. Contemporary Approaches for Site-Selective Dual Functionalization of Proteins. Angew Chem Int Ed Engl. 2021;60(25):13757-13777.

[3] Devabhaktuni A, Lin S, Zhang L, et al. TagGraph reveals vast protein modification landscapes from large tandem mass spectrometry datasets. Nat Biotechnol. 2019;37(4):469-479.

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