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Chemokine family proteins

Chemokine family proteins
Introduction
Schematic diagram of IGF-targeted drugs

Chemokine network in the tumoral microenvironment immune response

Chemokines are small cytokines or signaling proteins secreted by cells. According to the number and arrangement of conserved L-Cysteine (C) at the N-terminal, chemokines can be classified into four classes: C, CC, CXC and CX3C. These chemokines signal through the seven-pass transmembrane chemokine receptor of G-protein coupling on the cell surface, which stimulate the cell directional migration. Homeostatic chemokines are constitutively expressed under physiological conditions and play a role in cell migration and homing, whereas inflammatory chemokines are rapidly secreted at sites of inflammation, thereby recruiting effector cells into inflamed tissues. Thus, chemokines play a central role in the development and homeostasis of the immune system and are involved in promoting the activation, differentiation, proliferation and apoptosis of immune cells and in all protective or destructive immune and inflammatory responses.

Furthermore, chemokine signaling and the chemotaxis of various cell populations also play a central role in the tumor microenvironment (TME). In the TME, many chemokines are released by immune cells, tumor cells and tumor-associated cells. These chemokines can change with time and space, attracting different types of tumor-promoting and anti-tumor immune cells. The migration of immune cells will further affect the progression and metastasis of tumors, and directly affect the immune response. Studies have revealed that chemokines have pleiotropic functions that affect anti-tumor immune responses and tumor progression. With this in mind, therapeutic strategies that utilize or target chemokines have been established over the past decade, leading to the testing of many new drug candidates in clinical trials. Notably, in chemokine-based therapies, chemokines are highly redundant in binding to receptors, the same receptor can bind to different chemokines, and vice versa. Therefore, only by simultaneously inhibiting chemokine and its receptor with the same physiological function can the therapeutic effect be healthier and lasts longer.

ACROBiosystems has developed a series of CC, CXC and CX3C subfamily chemokine proteins expressed by HEK293. The products are available in a variety of species and labels. In addition, based on the “FLAG” technology platform, we developed full-length chemokine receptor such as CCR5, CCR8, CXCR3, CXCR4, CXCR5, etc. which with high biological activity was verified by binding to antibody. Our recombinant chemokine family proteins can accelerate your scientific research and drug development programs.

Product Features

Various molecules, species, and tags

High purity verified by SDS-PAGE

High structural homogeneity verified by SEC-MALS and DLS

High activity verified by ELISA/SPR

Highly stable and convenient for storage and transportation

Product List
Molecule Cat. No. Species Product Description Preorder/Order
CCL1 CC1-H82E9 Human Biotinylated Human CCL1 / I-309 / C-C motif chemokine 1 Protein, His,Avitag™ (MALS verified)

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CC1-H5254 Human Human CCL1 / I-309 Protein, Fc Tag (MALS verified)

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CCL2 CC2-H5255 Human Human CCL2 / MCP-1 Protein, Fc Tag (MALS verified)

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CCL3 CC3-H5249 Human Human CCL3 / MIP-1 alpha Protein, His Tag

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CCL5 CC5-H5251 Human Human CCL5/RANTES Protein, Fc Tag (MALS verified)

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CCL7 CC7-H52H6 Human Human CCL7 / MCP-3 Protein, His Tag

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CCL14 CC4-H5249 Human Human CCL14 / HCC-1 Protein, His Tag

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CCL17 CC7-H5256 Human Human CCL17 / TARC Protein, Fc Tag (MALS verified)

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CCL20 CC0-M82E5 Mouse Biotinylated Mouse CCL20 / MIP-3 alpha Protein, His,Avitag™

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CCL22 CC2-H5257 Human Human CCL22 / MDC Protein, Fc Tag

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CCL24 CC4-H52H1 Human Human CCL24/Eotaxin-2 Protein, His Tag

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CXCL1 CX1-H5253 Human Human CXCL1 Protein, Fc Tag

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CXCL4 CX4-H52H9 Human Human CXCL4 / PF4 Protein, His Tag

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CXCL10 CX0-H51H1 Human Human CXCL10 / IP-10 Protein, His Tag

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CXCL13 CX3-H1249 Human Human CXCL13 / BCA-1 Protein, His Tag

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CXCL16 CX6-H5254 Human Human CXCL16 Protein, Fc Tag

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CX3CL1 CX1-H5221 Human Human CX3CL1 / Fractalkine Protein, His Tag

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CX1-H8221 Human Biotinylated Human CX3CL1 Protein, His Tag, ultra sensitivity (primary amine labeling)

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CX1-C5222 Cynomolgus Cynomolgus CX3CL1 / Fractalkine Protein, His Tag

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CXCR3 CX3-H52P4 Human Human CXCR3 Full Length Protein-VLP (HEK293)

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CXCR4 CX4-H5219 Human Human CXCR4 / CD184 Full Length Protein-VLP (HEK293)

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CX4-H5269 Human Human CXCR4 / CD184 Protein, Fc Tag

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CXCR5 CX5-H52P4 Human Human CXCR5 Full Length Protein-VLP (HEK293)

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CCR5 CC5-H52P3 Human Human CCR5 Full Length Protein-VLP (HEK293)

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CC5-H52D1 Human Human CCR5 Protein, Flag,His Tag

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CHEK-ATP043 Human HEK293/Human CCR5 Stable Cell Line

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CCR8 CC8-H52P4 Human Human CCR8 Full Length Protein-VLP (HEK293)

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Verification Data
High purity and structural homogeneity verified by SDS-PAGE, SEC-MALS and DLS
CC5-H5251

The purity of Human CCL5, Fc Tag (Cat. No. CC5-H5251) is more than 85% and the molecular weight of this protein is around 71-96 kDa verified by SEC-MALS.

CX3-H52P4

The mean peak Radius of VLP (Cat. No. CX3-H52P4) is 60-80 nm with more than 95% intensity as determined by dynamic light scattering (DLS).

High biological activity verified by ELISA

CC1-H82E9

Immobilized Biotinylated Human CCL1, His,Avitag (Cat. No. CC1-H82E9) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human CCR8, Flag,His Tag with a linear range of 0.3-39 ng/mL (QC tested).

CX5-H52P4

Immobilized Human CXCR5 Full Length Protein-VLP (Cat. No. CX5-H52P4) at 5 μg/mL (100 μL/well) can bind Monoclonal Anti-Human CXCR5 antibody, Human IgG4 | Human Kappa with a linear range of 1-78 ng/mL (QC tested).

High affinity verified by SPR

CX3-H52P4

Anti-CXCR3 antibody immobilized on CM5 Chip can bind Human CXCR3 Full Length Protein-VLP (Cat. No. CX3-H52P4) with an affinity constant of 60.9 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).

CC5-H52D1

Human CCR5, Flag,His Tag (Cat. No. CC5-H52D1) captured on CM5 chip via anti-His antibody can bind Anti-CCR5 antibody (Human IgG1) with an affinity constant of 0.0737 nM as determined in a SPR assay (in presence of DDM and CHS) (Biacore T200).

References
  • 1. Kohli K, Pillarisetty V G, Kim T S. Key chemokines direct migration of immune cells in solid tumors[J]. Cancer gene therapy, 2022, 29(1): 10-21. https://doi.org/10.1038/s41417-021-00303-x.
  • 2. Bule P, Aguiar S I, Aires-Da-Silva F, et al. Chemokine-directed tumor microenvironment modulation in cancer immunotherapy[J]. International Journal of Molecular Sciences, 2021, 22(18): 9804. https://doi.org/10.3390/ijms22189804.
  • 3. Hughes C E, Nibbs R J B. A guide to chemokines and their receptors[J]. The FEBS journal, 2018, 285(16): 2944-2971.https://doi.org/10.1111/febs.14466.
  • 4. Chow M T, Luster A D. Chemokines in cancer[J]. Cancer immunology research, 2014, 2(12): 1125-1131.https://doi.org/10.1158/2326-6066.CIR-14-0160.

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