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Immune Checkpoint Proteins

Immune Checkpoint Proteins
Background

lmmune checkpoint proteins are key regulators of immune response against threats while preserving self-tolerance. The classic immune checkpoint consists of co-inhibitory receptors (CTLA-4, PD-1, TIGIT, LAG-3), which inhibit T-cell function and co-stimulatory receptors (GITR, 4-1BB, OX40, CD40) which stimulate T cell activation. Research on the co-inhibitory receptor CTLA-4 (cytotoxic T-lymphocyte antigen 4) and PD-1 (programmed cell death protein 1) showed significantly improved survival rates of patients with metastatic solid tumors after inhibition of these receptor pathways. Therefore, antibody drugs against immune checkpoints for cancer, tumors and other diseases therapeutics is a key mode in cancer therapy.

Pathways of Immune Checkpoint
CD155 ICOSL CD86 CD80 MHC CD40 PD-L2 PD-L1 4-1BBL OX40L GITRL MHC PD-L1 Galectin 9 TIGIT ICOS CTLA-4 CD28 TCR CD40L PD-1 PD-1 4-1BB OX40 GITR TCR PD-1 TIM-3 TCR PD-1 PD-1 LAG-3 MHC PD-L1 PD-L2 MHC-LL Dendritic cell Tumor cell Macrophage T-cells

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Stimulatory signal

Inhibitory signal

Click to learn more immune checkpoint molecules

Product Features & Applications
Product Features:
High purity verified by MALS or HPLCHigh bioactivity verified by ELISA/SPR/FACS, etcHigh batch to batch consistency by strict QC tested
Main Applications:
Product List

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  • Co-inhibitory receptors

  • Co-stimulatory receptors

  • Others

  • Inhibitor screening kits

MoleculesSpeciesTagsConjugates Features
(Available protocols)
PD-1Human, Mouse, Canine, Cynomolgus, Rabbit, Rat, Rhesus macaqueFc, His, Fc & His, Fc & Avi & His, Fc & Avi, His & Avi, mFc& Avi, Twin-Strep, Llama Fc , mFcBiotin-labeled, PE-labeled, UnconjugatedSDS-PAGE, MALS, HPLC, ELISA, BLI, SPR, FACS
PD-L1Human, Mouse, Rabbit, Canine, Cynomolgus / Rhesus macaqueFc, His, Fc & Avi, His & Avi, Fc & Avi & His, mFc, LlamaFc, mFc& Avi, Twin-StrepBiotin-labeled, UnconjugatedSDS-PAGE, MALS, ELISA, BLI, SPR, FACS
PD-L2Human, MouseFc, His, Fc & Avi, Avi & His, mFcBiotin-labeled, UnconjugatedSDS-PAGE, HPLC, ELISA, SPR, BLI, FACS, Cell based assay
B7-H3Human, Mouse, CynomolgusFc & Avi, Avi & His, His, Fc, Avi & HisBiotin-labeled, UnconjugatedSDS-PAGE, MALS, ELISA, Cell based assay
B7-H4Human, MouseHis, FcBiotin-labeled, UnconjugatedSDS-PAGE, ELISA
B7-H5Human, Mouse, Rhesus macaqueFc & Avi, Avi & His, His, Fc, mFcBiotin-labeled, UnconjugatedSDS-PAGE, MALS, ELISA, SPR, BLI
BTLAHuman, MouseFc & Avi, His & Avi, His, mFc, FcBiotin-labeled, UnconjugatedSDS-PAGE, ELISA
CD80(B7-1)Human, Mouse, Cynomolgus / Rhesus macaqueFc, Fc & Avi, Avi & His, mFc, HisBiotin-labeled, UnconjugatedSDS-PAGE, MALS, HPLC, ELISA, SPR, BLI, FACS
CD86(B7-2)Human, Mouse, Cynomolgus / Rhesus macaqueFc & Avi, Fc, HisBiotin-labeled, UnconjugatedSDS-PAGE, MALS, HPLC, ELISA, BLI, FACS
CD96HumanHis & Avi, His, mFcBiotin-labeled, UnconjugatedSDS-PAGE, MALS, ELISA, BLI, FACS
CD47Human, Mouse, Rabbit, Rat, Cynomolgus / Rhesus macaque, CanineFc, His, Fc & Avi, His & Avi, mFc, mFc & Avi, LlamaFcBiotin-labeled, FITC-labeled, UnconjugatedSDS-PAGE, MALS, ELISA, SPR, BLI, FACS
CD155Human, Mouse, Rhesus macaqueFc & Avi, His & Avi, mFc, Fc, HisBiotin-labeled, UnconjugatedSDS-PAGE, MALS, ELISA, BLI, FACS
CTLA-4Human, Mouse, Rabbit, Rat, Cynomolgus / Rhesus macaqueFc, His, Fc & Avi, His & Avi, mFc, mFc& AviBiotin-labeled, UnconjugatedSDS-PAGE, MALS, ELISA, SPR, BLI, FACS
LAG-3Human, Mouse, Rat, Cynomolgus, MarmosetFc, His, Fc & Avi, His & Avi, mFc, mFc & Avi, LlamaFcBiotin-labeled, UnconjugatedSDS-PAGE, MALS, ELISA, SPR, BLI, FACS
TIGITHuman, Mouse, Rabbit, Rat, Cynomolgus / Rhesus macaque, CaninemFc& Avi, Fc & Avi, His & Avi, His, Fc, mFcBiotin-labeled, UnconjugatedSDS-PAGE, MALS, HPLC, ELISA, SPR, BLI
TIM-3Human, Mouse, CynomolgusAvi & His, mFc, Fc, HisBiotin-labeled, UnconjugatedSDS-PAGE, MALS, ELISA, SPR
CD111HumanHis, FcUnconjugatedSDS-PAGE, ELISA, BLI
DNAM-1Human, CynomolgusFc & Avi, His, FcBiotin-labeled, UnconjugatedSDS-PAGE, ELISA, BLI, FACS
Galectin-9HumanHis, MBP & HisUnconjugatedSDS-PAGE
HVEMHumanHis, Twin-Strep, Fc, mFc, Fc & AviBiotin-labeled, Unconjugated ,SDS-PAGE, ELISA, BLI
Nectin-2HumanHis, FcBiotin-labeled, UnconjugatedSDS-PAGE, HPLC, ELISA, BLI
Nectin-3HumanHis, FcBiotin-labeled, UnconjugatedSDS-PAGE, MALS, ELISA, BLI
PVRIGHuman, Mouse, CynomolgusFc & Avi, mFc& Avi, His & Avi, His, mFcBiotin-labeled, UnconjugatedSDS-PAGE, MALS, ELISA, BLI
SIRP alphaHuman, Mouse, CynomolgusFc, His & Avi, mFc& Avi, mFc, HisBiotin-labeled, PE-labeled, UnconjugatedSDS-PAGE, MALS, HPLC, ELISA, BLI, FACS
SIRP alpha V2HumanHisBiotin-labeled, UnconjugatedSDS-PAGE, ELISA
SIRP gammaHumanFcUnconjugatedSDS-PAGE, ELISA
CD160HumanHisUnconjugatedSDS-PAGE, ELISA
MoleculesSpeciesTagsConjugates Features
(Available protocols)
4-1BB LigandHuman, Mouse, Rat, CynomolgusFc, Fc & Avi, mFc, His & FlagBiotin-labeled, UnconjugatedSDS-PAGE, MALS, ELISA, BLI, Cell based assay
4-1BBHuman, Mouse, Rat, Rabbit, Cynomolgus / Rhesus macaque, CanineHis, Fc, Fc & Avi, His & Avi, mFcBiotin-labeled, UnconjugatedSDS-PAGE, MALS, ELISA, SPR
OX40 LigandHuman, Mouse, Rhesus macaqueFc, His, His & Avi, Avi & Fc, mFcBiotin-labeled, UnconjugatedSDS-PAGE, MALS, ELISA, BLI, FACS
OX40Human, Mouse, Rabbit, Rat, Rhesus macaque, MarmosetHis, Fc, His & Avi, Fc & Avi, Avi & His, mFcBiotin-labeled, UnconjugatedSDS-PAGE, MALS, ELISA, BLI, FACS
ICOS Ligand
(B7-H2)
Human, Mouse, CynomolgusHis, FcUnconjugatedSDS-PAGE, ELISA
ICOSHuman, Mouse, CynomolgusHis, Fc, His & AviBiotin-labeled, UnconjugatedSDS-PAGE, ELISA
GITR LigandHuman, MouseHis, Fc, Avi & FcBiotin-labeled, UnconjugatedSDS-PAGE, ELISA
GITRHuman, Mouse, Rat, Rhesus macaque, CanineHis, Fc, His & Avi, Fc & Avi, mFcBiotin-labeled, UnconjugatedSDS-PAGE, MALS, ELISA
CD48HumanHis, Fc, mFcUnconjugatedSDS-PAGE, ELISA
HVEMHumanHis, Fc, Fc & Avi, mFc, TwiStrepBiotin-labeled, UnconjugatedSDS-PAGE, MALS, BLI
Nectin-2HumanHis, Fc, Fc & Avi, mFcBiotin-labeled, UnconjugatedSDS-PAGE, MALS, HPLC, ELISA, BLI
CD40 LigandHuman, Mouse, Human / Rhesus macaqueHis, Fc, His & Avi, Avi & Fc, Avi & His & Flag, His & Flag, mFcBiotin-labeled, UnconjugatedSDS-PAGE, MALS, ELISA, BLI
CD40Human, Mouse, Rabbit, Cynomolgus, Rhesus macaqueHis, Fc, Avi & His, Fc & Avi, mFcBiotin-labeled, UnconjugatedSDS-PAGE, MALS, ELISA, BLI, FACS
CD30 ligandHumanHis, mFcUnconjugatedSDS-PAGE, ELISA, BLI, FACS
CD30HumanHis, Fc, Avi & His, LlamaFcBiotin-labeled, PE-labeled, FITC-labeled, UnconjugatedSDS-PAGE, MALS, ELISA, BLI, FACS
CD28Mouse, Human / Cynomolgus / Rhesus macaqueHis, Fc, His & Avi, Fc & Avi, mFcBiotin-labeled, UnconjugatedSDS-PAGE, HPLC, ELISA, SPR, BLI
CD27 LigandHuman, MouseHis & Avi, mFc, His, His & Avi & Flag, His & Flag, FcBiotin-labeled, FITC-labeled, UnconjugatedSDS-PAGE, MALS, HPLC, ELISA
CD27Human, MouseHis, Fc, Fc & Avi, mFcBiotin-labeled, UnconjugatedSDS-PAGE, MALS, HPLC, ELISA
B7-1Human, Mouse, Cynomolgus / Rhesus macaqueFc, Fc & Avi, Avi & His, mFc, HisBiotin-labeled, UnconjugatedSDS-PAGE, MALS, HPLC, ELISA, SPR, BLI, FACS
B7-2Human, Mouse, Cynomolgus / Rhesus macaqueHis, Fc, Fc & AviBiotin-labeled, UnconjugatedSDS-PAGE, MALS, HPLC, ELISA, BLI, FACS
LIGHTHumanHis, mFc, FcUnconjugatedSDS-PAGE, MALS, ELISA, BLI
B7-H7Human, CynomolgusHis, Fc, His & Avi, Fc & AviBiotin-labeled, UnconjugatedSDS-PAGE, MALS, BLI
B7-H6Human, CynomolgusHis, His & Avi, Fc & AviBiotin-labeled, UnconjugatedSDS-PAGE, ELISA, SPR
2B4HumanHis, Fc, Avi & HisBiotin-labeled, UnconjugatedSDS-PAGE, ELISA
CD28HHumanHis, FcUnconjugatedSDS-PAGE, ELISA
NKp30HumanHis, FcUnconjugatedSDS-PAGE, SPR
Key Features

The protocols are available for free.

Authentic structure verified by SEC-MALS

Figure 1. The purity of Human CD27 Ligand, His,Flag Tag (active trimer) (MALS verified) (Cat. No. CDL-H52Da) was more than 95% in HP-SEC, and around 55-70 kDa verified by SEC-MALS.

High batch to batch consistency

The consistency in purity and bioactivity is essential for recombinant proteins.All our proteins have to pass a variety of purity and bioactivity assessments.

Biotinylated Human PD-1 / PDCD1 Protein, Avitag™,His Tag (recommended for biopanning) (MALS verified)(Cat. No. PD1-H82E4

Application 1: Development and screening of antibody drugs

Antibody binding assay

PD-1&PD-L1 pathway

Immobilized Human PD-1, His Tag (Cat. No. PD1-H5221) at 2 μg/mL (100 μL/well) can bind Nivolumab with a linear range of 0.1-3 ng/mL (Routinely tested).                  

PD-1&PD-L1 pathway

Serial dilutions of nivolumab were added into Human PD-1, Fc Tag, low endotoxin (Cat. No. PD1-H5257): Biotinylated Human PD-L1, Fc, Avitag, His Tag (Cat. No. PD1-H82F3) binding reactions. The half maximal inhibitory concentration (IC50) is 0.5381 μg/mL (Routinely tested).

In addition, ACROBiosystems have also developed PD1: PD-L1 (Cat. No. EP-101) and CD47: SIRP alpha (Cat. No. EP-102) inhibitor screening assay kits that can screen neutralizing antibodies or small molecules with high throughput.

Learn more about inhibitor screening kit

Antibody drug affinity assay

LAG-3 pathway

Anti-LAG-3 MAb (Human IgG1) captured on CM5 chip via Anti-Human IgG Fc antibodies surface, can bind Human LAG-3, His Tag (Cat. No. LA3-H5222) with an affinity constant of 4.57 nM as determined in a SPR assay (Biacore T200) (Routinely tested).

LAG-3 pathway

Loaded Anti-LAG-3 MAb (Human IgG1) on AHC Biosensor, can bind Human LAG-3, His Tag (Cat. No. LA3-H5222) with an affinity constant of 7.47 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Neutralizing antibody binding assay

TIGIT pathway

Serial dilutions of Human TIGIT Neutralizing antibody were added into Human TIGIT, Fc Tag (Cat. No. TIT-H5254): Biotinylated Human CD155, Fc,Avitag (Cat. No. CD5-H82F6) binding reactions. The half maximal inhibitory concentration (IC50) is 0.55834 μg/mL (Routinely tested).

CD47&SIPR α pathway(Cell-based assay)

FACS analysis shows that the binding of Human CD47 to ACHN expressing SIRP-a was inhibited by increasing concentration of neutralizing SIRP-a antibody. The concentration of Human CD47 used is 20 μg/mL. IC50=9.334 μg/mL

Cell-based assay

4-1BB pathway

Human 4-1BB Ligand (71-254), Fc Tag, active trimer (Cat. No. 41L-H5265) induce IL-8 secretion in HT1080 human CD137 cell line. The EC50 for this effect is 0.079 μg/mL (Routinely tested).


Pharmacokinetics (PK) analysis

CTLA-4 pathway

Immobilized Human CTLA-4, His Tag, active dimer (Cat. No. CT4-H52H9) at 2 μg/mL, add increasing concentrations of CTLA-4 x OX40 Bispecific Antibody in 50% Human serum and then add Biotinylated Human OX40, Avitag, His Tag(Cat. No. TN4-H82E4) at 1 μg/mL. Detection was performed using HRP-conjugated streptavidin with sensitivity of 4 ng/mL (Intact assay, Routinely tested).

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