Organoids are three-dimensional tissue structures composed of a variety of cell types. Through in vitro 3D cell culture, they can continually be expanded in vitro for a long time and retain the key characteristics of organs. It can simulate the architectural and functional characteristics of human organs and even the tumor microenvironment. With recent advancements in organoid culture and the elucidation of growth factors needed to direct cell differentiation, organoids have become an indispensable tool in the process of drug development and evaluation. Currently, most organs in the human body can be formed into organoids in vitro, reproducing the cellular milleau of liver, lung, heart, blood vessels, and many others. As such, organoids have the potential to become an essential preclinical model for human disease research and drug development.
To support research on organoid cell culturing, ACROBiosystems has developed a series of high-quality ActiveMax® cytokines, including EGF, Noggin, R-Spondin 1, FGF10, FGF2, and Activin A. These products are suitable as growth factors for organoid culture that have been verified to promote organoid growth.
Human EGF (Cat. EGF-H52H3), Noggin (Cat. NON-H5257) and R-spondin1 (Cat.RS6-H4220) could support the growth of mouse intestinal organoids.
Human FGF basic (Cat. No. BFF-H4117) stimulates proliferation of HUVEC in the range of 0-20 ng/mL. The EC50 for this effect is 0.416-0.630 ng/mL (Routinely tested).
The bio-activity of Human Activin A (Cat. No. ACA-H421b) was determined by dose-dependent inhibition of the proliferation of MPC-11 cells. The EC50 for this effect is typically ≤ 9.5 ng/mL (Routinely tested).
Human BMP-2, Tag Free(Cat. No. BM2-H4117) induces alkaline phosphatase production in ATDC5 cells. The EC50 for this effect is 1684-1736 ng/ml (Routinely tested).
The bio-activity of Human PDGF-BB Protein (Cat. No. PDB-H4112) was determined by dose-dependent stimulation of the proliferation of mouse 3T3 cells. The ED50 was 0.5-20 ng/mL (Routinely tested).