0
There is no goods in the shopping cart !
A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 0-9
Your Position: Home > Antigens & Antibodies for Diagnostic Kit Development

Antigens & Antibodies for Diagnostic Kit Development

   Committed to high sensitivity & specificity

 Antigens & Antibodies for Diagnostic kit development – Dedicated to high sensitivity & specificity
Background

The current methods of detecting SARS-CoV-2 infection include nucleic acid detections, antibody detections, and antigen detections. Compared to nucleic acid detections, antibody detections and antigen detections are faster and more convenient. Therefore, they are suitable for scenarios such as rapid screening and instant detection.

The sensitivity and specificity of the serological diagnostic kits are significantly affected by the quality of antigen and antibody reagents. To better support the development of the diagnostic kits, ACROBiosystems has developed a serial of high-quality antigen and antibody reagents. Furthermore, several in vitro diagnostics companies are using ACRO's reagents to develop COVID-19 serological diagnostics products. Among them, Babson Diagnostics, a US diagnostics company, successfully obtained the Emergency Use Authority from FDA and launched their chemiluminescent COVID-19 antibody detection kit employing ACRO's biotin-labeled S1 protein.

Matched antibody pairs

ACROBiosystems has launched matched antibody pairs that are specifically designed and screened for antigen detection kit development, which are suitable for different diagnostic assays, such as chemiluminescent immunoassay, ELISA, gold immunochromatography assay, etc. The sensitivity of the sandwich ELISA can be as low as 12 pg/mL in the verification study using recombinant antigens. In addition, the antibody pairs have been verified using inactivated virus samples. The titer, which was determined using our LFA assay, is as low as 102CCID50.

Product list

We now offer free samples to the researchers working on the diagnostic kit development. Get it now!

CategoryCat. No.SpeciesProduct Description
Nucleocapsid proteinNUN-S46MouseAnti-SARS-CoV-2 Nucleocapsid Antibody, Mouse IgG1 (AS46) (Trehalose free)Inquiry
NUN-S47MouseAnti-SARS-CoV-2 Nucleocapsid Antibody, Mouse IgG1 (AS47) (Trehalose free)Inquiry
NUN-S57MouseAnti-SARS-CoV-2 Nucleocapsid Antibody, Mouse IgG1 (AS57) (Trehalose free)Inquiry
NUN-S60MouseAnti-SARS-CoV-2 Nucleocapsid Antibody, Mouse IgG1 (AS60) (Trehalose free)Inquiry
NUN-S61MouseAnti-SARS-CoV-2 Nucleocapsid Antibody, Mouse IgG1 (AS61) (Trehalose free)Inquiry
S1 proteinS1N-M12A1MouseAnti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 (AM122) (Trehalose free)Inquiry
S1N-M13A1MouseAnti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 (AM130) (Trehalose free)Inquiry
S1N-S58MouseAnti-SARS-CoV-2 Spike S1 Antibody, Mouse IgG1 (AS58) (Trehalose free)Inquiry
S1N-S59MouseAnti-SARS-CoV-2 Spike S1 Antibody, Mouse IgG1 (AS59) (Trehalose free)Inquiry

These antibodies were recommended for antigen detection by double antibody sandwich.

>>>  Virus lysis buffer (Cat.No. LY-13) is available for antigen detection kit development.
>>>  If you have any bulk order inquiry, please feel free to click here.

Assay data

Verification study using inactivated virus

Fig1.  The titer is as low as 102CCID50 in the verification study using inactivated virus.

Related article: COVID-19 anti-nucleocapsid antibodies verified with inactivated virus samples

High sensitivity of 12 pg/mL (ELISA assay)

Fig 2 Immobilized Anti- SARS-CoV-2 Nucleocapsid antibody, mouse Mab (Cat. No. NUN-S46) at 4 μg/mL, add increasing concentrations of SARS-CoV-2 Nucleocapsid protein and then add Biotinylated Anti- SARS-CoV-2 Nucleocapsid antibody, mouse Mab (Cat. No. NUN-S47). Detection was performed using HRP-conjugated streptavidin with sensitivity of 12 pg/mL.

Super affinity of 0.01 nM level(BLI assay)

Fig 3 Anti-SARS-CoV-2 Nucleocapsid Antibody, Mouse MAb(Cat. No. NUN-S47) can bind SARS-CoV-2 Nucleocapsid Protein(Cat. No. NUN-C5227) with an affinity constant of 24.7pM determined in BLI assay (ForteBio Octet Red96e)


Specific binding to SARS-CoV-2 Nucleocapsid protein

Fig 4 Demonstration of the specificity of Anti-SARS-CoV-2 N antibody pairs (Cat. No. NUN-S46 & Cat. No. NUN-S47) to the Nucleocapsid protein.

High batch-to batch consistency

Fig 5 Demonstration of the Batch to Batch Consistency of Anti-SARS-CoV-2 Spike S1 Antibody, Chimeric Mab (Cat. No. S1N-M122) to the S1 protein.

Antigens

ACROBiosystems has designed Spike and Nucleocapsid antigens specifically for diagnostic kits development. The antigens are customizable and bulk order is available. They are perfect for different antibody detection methods, such as chemiluminescent immunoassay, ELISA, gold immunochromatography assay, etc.

Product list

MoleculeCat. No.HostTagRecommended applicationRecommended method
S proteinSPN-C52H7HEK293His TagDetect IgG/IgM by capture methodELISA
GICA
CLIA
Inquiry
S1 protienS1N-C52H2HEK293His TagDetect IgG/IgM by capture methodInquiry
S1N-C82E8HEK293His,Avi tag™Detect IgG/IgM by capture methodInquiry
S2 proteinS2N-C52H2HEK293His TagDetect IgG/IgM by capture methodInquiry
RBD proteinSPD-C52H1HEK293His TagDetect total antibodies by double antigen sandwich method (detection)Inquiry
SPD-C52H2HEK293His TagDetect IgG/IgM by capture methodInquiry
SPD-C5251HEK293Mouse Fc TagDetect total antibodies by double antigen sandwich method (capture)Inquiry
SPD-C82E9HEK293His,Avi tag™Detect IgG/IgM by capture methodInquiry
Nucleocapsid proteinNUN-C5221HEK293His TagDetect IgG/IgM by capture methodInquiry
NUN-C51H2E.coliHis TagDetect IgG/IgM by capture methodInquiry
NUN-C81Q8E.coliHis,Avi tag™Detect IgG/IgM by capture methodInquiry
Envelope ProteinENN-C5128HEK293GST,His TagDetect IgG/IgM by capture methodInquiry

>>>  If you have any bulk order inquiry, please feel free to click here.

>>>  If you have any custom inquiries or suggestions for SARS-CoV-2 (COVID-19) related products, please click here.

IgG/IgM control antibodies are available for antibody detection kit development.

CategoryCat. No.SpeciesProduct Description
S protein RBDSAD-S35HumanAnti-SARS-CoV-2 Spike RBD Neutralizing Antibody, Human IgG1 (AS35)Inquiry
SPD-M128MouseAnti-SARS-CoV-2 RBD Potent Neutralizing Antibody, Mouse/Human Chimeric mAb, Human IgG1 (AM128)Inquiry
SPD-M141HumanAnti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgM (AM130)Inquiry

Assay data

High sensitivity of 0.02 ng/mL (ELISA assay)

Fig.6 Immobilized SARS-CoV-2 Nucleocapsid protein, His Tag at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 NP Antibody, Rabbit MAb (CLN27) with a linear range of 0.02-0.3 ng/mL.

>>> All SARS-CoV-2 related products

>>> SARS-CoV-2 related kits

>>> Pre-coupling beads

>>> Any custom requests, suggestions or feedback are welcome.Click here.

This web search service is supported by Google Inc.

totop