A-Z> Antigens & Antibodies for Diagnostic Kit Development
The current methods of detecting SARS-CoV-2 infection include nucleic acid detections, antibody detections, and antigen detections. Compared to nucleic acid detections, antibody detections and antigen detections are faster and more convenient. Therefore, they are suitable for scenarios such as rapid screening and instant detection.
The sensitivity and specificity of the serological diagnostic kits are significantly affected by the quality of antigen and antibody reagents. To better support the development of the diagnostic kits, ACROBiosystems has developed a serial of high-quality antigen and antibody reagents. Furthermore, several in vitro diagnostics companies are using ACRO's reagents to develop COVID-19 serological diagnostics products. Among them, Babson Diagnostics, a US diagnostics company, successfully obtained the Emergency Use Authority from FDA and launched their chemiluminescent COVID-19 antibody detection kit employing ACRO's biotin-labeled S1 protein.
ACROBiosystems has launched matched antibody pairs that are specifically designed and screened for antigen detection kit development, which are suitable for different diagnostic assays, such as chemiluminescent immunoassay, ELISA, gold immunochromatography assay, etc. The sensitivity of the sandwich ELISA can be as low as 12 pg/mL in the verification study using recombinant antigens. In addition, the antibody pairs have been verified using inactivated virus samples. The titer, which was determined using our LFA assay, is as low as 102CCID50.
We now offer free samples to the researchers working on the diagnostic kit development. Get it now!
| Category | Cat. No. | Species | Product Description | |
|---|---|---|---|---|
| Nucleocapsid protein | NUN-S46 | Mouse | Anti-SARS-CoV-2 Nucleocapsid Antibody, Mouse IgG1 (AS46) (Trehalose free) | Inquiry |
| NUN-S47 | Mouse | Anti-SARS-CoV-2 Nucleocapsid Antibody, Mouse IgG1 (AS47) (Trehalose free) | Inquiry | |
| NUN-S57 | Mouse | Anti-SARS-CoV-2 Nucleocapsid Antibody, Mouse IgG1 (AS57) (Trehalose free) | Inquiry | |
| NUN-S60 | Mouse | Anti-SARS-CoV-2 Nucleocapsid Antibody, Mouse IgG1 (AS60) (Trehalose free) | Inquiry | |
| NUN-S61 | Mouse | Anti-SARS-CoV-2 Nucleocapsid Antibody, Mouse IgG1 (AS61) (Trehalose free) | Inquiry | |
| S1 protein | S1N-M12A1 | Mouse | Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 (AM122) (Trehalose free) | Inquiry |
| S1N-M13A1 | Mouse | Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgG1 (AM130) (Trehalose free) | Inquiry | |
| S1N-S58 | Mouse | Anti-SARS-CoV-2 Spike S1 Antibody, Mouse IgG1 (AS58) (Trehalose free) | Inquiry | |
| S1N-S59 | Mouse | Anti-SARS-CoV-2 Spike S1 Antibody, Mouse IgG1 (AS59) (Trehalose free) | Inquiry |
These antibodies were recommended for antigen detection by double antibody sandwich.
>>> Virus lysis buffer (Cat.No. LY-13) is available for antigen detection kit development.
>>> If you have any bulk order inquiry, please feel free to click here.
Fig1. The titer is as low as 102CCID50 in the verification study using inactivated virus.
Related article: COVID-19 anti-nucleocapsid antibodies verified with inactivated virus samples
Fig 2 Immobilized Anti- SARS-CoV-2 Nucleocapsid antibody, mouse Mab (Cat. No. NUN-S46) at 4 μg/mL, add increasing concentrations of SARS-CoV-2 Nucleocapsid protein and then add Biotinylated Anti- SARS-CoV-2 Nucleocapsid antibody, mouse Mab (Cat. No. NUN-S47). Detection was performed using HRP-conjugated streptavidin with sensitivity of 12 pg/mL.
Fig 4 Demonstration of the specificity of Anti-SARS-CoV-2 N antibody pairs (Cat. No. NUN-S46 & Cat. No. NUN-S47) to the Nucleocapsid protein.
Fig 5 Demonstration of the Batch to Batch Consistency of Anti-SARS-CoV-2 Spike S1 Antibody, Chimeric Mab (Cat. No. S1N-M122) to the S1 protein.
ACROBiosystems has designed Spike and Nucleocapsid antigens specifically for diagnostic kits development. The antigens are customizable and bulk order is available. They are perfect for different antibody detection methods, such as chemiluminescent immunoassay, ELISA, gold immunochromatography assay, etc.
| Molecule | Cat. No. | Host | Tag | Recommended application | Recommended method | |
|---|---|---|---|---|---|---|
| S protein | SPN-C52H7 | HEK293 | His Tag | Detect IgG/IgM by capture method | ELISA GICA CLIA | Inquiry |
| S1 protien | S1N-C52H2 | HEK293 | His Tag | Detect IgG/IgM by capture method | Inquiry | |
| S1N-C82E8 | HEK293 | His,Avi tag™ | Detect IgG/IgM by capture method | Inquiry | ||
| S2 protein | S2N-C52H2 | HEK293 | His Tag | Detect IgG/IgM by capture method | Inquiry | |
| RBD protein | SPD-C52H1 | HEK293 | His Tag | Detect total antibodies by double antigen sandwich method (detection) | Inquiry | |
| SPD-C52H2 | HEK293 | His Tag | Detect IgG/IgM by capture method | Inquiry | ||
| SPD-C5251 | HEK293 | Mouse Fc Tag | Detect total antibodies by double antigen sandwich method (capture) | Inquiry | ||
| SPD-C82E9 | HEK293 | His,Avi tag™ | Detect IgG/IgM by capture method | Inquiry | ||
| Nucleocapsid protein | NUN-C5221 | HEK293 | His Tag | Detect IgG/IgM by capture method | Inquiry | |
| NUN-C51H2 | E.coli | His Tag | Detect IgG/IgM by capture method | Inquiry | ||
| NUN-C81Q8 | E.coli | His,Avi tag™ | Detect IgG/IgM by capture method | Inquiry | ||
| Envelope Protein | ENN-C5128 | HEK293 | GST,His Tag | Detect IgG/IgM by capture method | Inquiry |
>>> If you have any bulk order inquiry, please feel free to click here.
>>> If you have any custom inquiries or suggestions for SARS-CoV-2 (COVID-19) related products, please click here.
IgG/IgM control antibodies are available for antibody detection kit development.
| Category | Cat. No. | Species | Product Description | |
|---|---|---|---|---|
| S protein RBD | SAD-S35 | Human | Anti-SARS-CoV-2 Spike RBD Neutralizing Antibody, Human IgG1 (AS35) | Inquiry |
| SPD-M128 | Mouse | Anti-SARS-CoV-2 RBD Potent Neutralizing Antibody, Mouse/Human Chimeric mAb, Human IgG1 (AM128) | Inquiry | |
| SPD-M141 | Human | Anti-SARS-CoV-2 Spike RBD Antibody, Chimeric mAb, Human IgM (AM130) | Inquiry |
Fig.6 Immobilized SARS-CoV-2 Nucleocapsid protein, His Tag at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 NP Antibody, Rabbit MAb (CLN27) with a linear range of 0.02-0.3 ng/mL.
>>> All SARS-CoV-2 related products 
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