A-Z> SARS-CoV-2 Related Kit Products
SARS-CoV-2 is an enveloped positive-stranded RNA virus that consists of four structural proteins including spike (S) glycoprotein, envelope (E) protein, membrane (M) protein, and nucleocapsid (N) protein. Spike glycoprotein is the most important surface protein of SARS-CoV-2, which can mediate the virus entry into human respiratory epithelial cells by interacting with cell surface receptor angiotensin-converting enzyme 2 (ACE2). S, S1 and S RBD proteins are important targets in the development of SARS-COV-2 therapeutics and vaccines.
High-quality recombinant antigen proteins and kit products are critical in accelerating the research and development of anti-SARS-CoV-2 drugs and vaccines. ACROBiosystems has developed a series of kit products using its high-quality SARS-CoV-2 antigen proteins to improve the efficiency of anti-SARS-CoV-2 drugs and vaccines development. These kit products include SARS-CoV-2 inhibitor screening kits, antibody titer serologic assay kits, antigen detection kit, antigen pre-coated plates and these products can be used for SARS-CoV-2 inhibitor screening, neutralizing antibody screening, antibody detection, antigen detection.
SARS-CoV-2 inhibitor screening kit can be used to screen small molecule inhibitors, polypeptide inhibitors and neutralizing antibodies efficiently and rapidly.
High specificity > Biotinylated ACE2 protein was employed to ensure the high specificity
High reproducibility > The kit is produced under strict quality control system with high batch-to-batch consistency
High versatility > The kit can be used to screen a variety of antibodies and small molecule compound inhibitors
High Throughput > The package sizes of 96 tests and 480 tests make the simultaneous screening of a large number of samples available
High-efficiency > Fast and easy-to-use
Fig.1 Inhibition of Human ACE2 [Biotinylated]: SARS-CoV-2 S protein RBD Binding by SARS-CoV-2 inhibitor
Serial dilutions of SARS-CoV-2 inhibitor (Cat. No. AC2-NA005) (1:2 serial dilutions, from 20 μg/mL to 0.039 μg/mL (180 nM to 0.35 nM)) was added into Biotinylated ACE2: SARS-CoV-2 S protein RBD binding reactions. The assay was performed according to the above described protocol. Background was subtracted from data points prior to log transformation and curve fitting.
* Corresponding buffer set offered.（Cat.No. EBS-001 ELISA Buffer Set-96T）
ACROBiosystems has developed a series of antibody titer assay kit, including IgG antibody titer assay kit, IgM antibody titer assay kit, total antibody titer assay kit, total protective neutralizing antibody titer assay kit and so on. These antibody titer assay kits can be used for vaccine research and development, to verify the effectiveness of the vaccine by detecting the antibody titer, and also to evaluate the scientific research of antibody titer in infected patients. The results of neutralizing antibody titer indicate its neutralizing ability, which can be used as one of the criterions for antibody assessment.
Excellent performance > Developed by ACRO’s high-quality protein with high specificity, sensitivity and reproducibility
Wide diversity > different antigen coated plates to apply for different application
Fig.2 Detection of Monoclonal Anti-SARS-CoV-2 Antibody, Human IgG1 titer by Indirect-ELISA Assay
Immobilized SARS-CoV-2 S protein RBD at 0.2 μg/mL (100 μL/well) can bind Monoclonal Anti-SARS-CoV-2 Antibody, Human IgG1 in 1:50 human serum.Detection was performed using HRP-Conjugated Anti-human IgG antibody with sensitivity of 24 ng/mL.
Fig.3 Determination of Anti-SARS-CoV-2 Neutralizing Antibody titer in different concentrations of human serum.
Neutralizing titer of Anti-SARS-CoV-2 Neutralizing Antibody, Human IgG1 (Cat.No.SAD-S35) measured by Anti-SARS-CoV-2 neutralizing antibody titer serologic assay kit (Cat. No. TAS-K003) in different concentrations of human serum (CV < 30%).
Double antibody sandwich method was employed in the detection of SARS-COV-2 antigen protein. The antigen assay kit with high sensitivity can be used to determine the viral infection status and guide the design of vaccine development.
ACROBiosystems are developing antigen detection kit for SARS-CoV-2 S1 antigen protein and N antigen protein.
Fig.4 Detection SARS-CoV-2 Nucleocapsid Protein by ELISA Assay.
Immobilized Anti-SARS-CoV-2 Nucleocapsid Antibody at 4 μg/mL (100 μL/well) can bind SARS-CoV-2 Nucleocapsid Protein. And then add Biotinylated Anti-SARS-CoV-2 Nucleocapsid Antibody at 1:5000. Detection was performed using high sensitivity HRP-conjugated streptavidin with sensitivity of 12.5 pg/mL
Plates precoated with S1 and S RBD protein can be used to quantify anti-SARS-COV-2 antibodies or ACE2 protein. These plates with high sensitivity, good reproducibility and high throughput are suitable for neutralizing antibody screening, biopanning, ELISA detection and so on. They can help improve the efficiency of the research and development of anti-SARS-CoV-2 drugs and vaccines.
Fig.5 Binding with Anti-SARS-CoV-2 S1 antibody
Immobilized biotinylated SARS-CoV2 Spike protein RBD at 1 μg/mL (100 μL/well) on Streptavidin Coated Plates, Clear, 96-Well (Cat. No. SP-11), can bind anti-SARS-CoV-2 S1 antibody with a linear range of 0.1-2 ng/mL.
| Category | Molecule | Cat.No. | Product Description |
|---|---|---|---|
| Neutralizing antibody titer assay kit | S protein RBD | TAS-K003 | Anti-SARS-CoV-2 neutralizing antibody titer serologic assay kit |
| Antibody titer assay kit | S protein | TAS-K007 | Anti-SARS-CoV-2 Antibody IgG Titer Serologic Assay kit (S protein trimer) |
| S1 protein | TAS-K001 | Anti-SARS-CoV-2 Antibody IgG Titer Serologic Assay kit (S protein S1) | |
| S protein RBD | TAS-K002 | Anti-SARS-CoV-2 Antibody IgG Titer Serologic Assay kit (S protein RBD) | |
| Nucleocapsid protein | TAS-K008 | Anti-SARS-CoV-2 Antibody IgG Titer Serologic Assay kit (Nucleocapsid Protein) | |
| S protein | TAS-K009 | Anti-SARS-CoV-2 Total Antibody Titer Serologic Assay kit | |
| S1 protein | TAS-K012 | Anti-SARS-CoV-2 Antibody IgM Serologic Assay kit (S1 protein) | |
| S protein RBD | TAS-K013 | Anti-SARS-CoV-2 Antibody IgM Serologic Assay kit (Spike RBD protein) | |
| Antibody isotype assay kit | S protein RBD | TAS-K014 | Anti-SARS-CoV-2 IgG1 Titer Serologic Assay Kit (S Protein RBD) |
| S protein RBD | TAS-K015 | Anti-SARS-CoV-2 IgG2 Titer Serologic Assay Kit (S Protein RBD) | |
| S protein RBD | TAS-K016 | Anti-SARS-CoV-2 IgG3 Titer Serologic Assay Kit (S Protein RBD) | |
| S protein RBD | TAS-K017 | Anti-SARS-CoV-2 IgG4 Titer Serologic Assay Kit (S Protein RBD) | |
| Antigen assay kit | Nucleocapsid Protein | TAS-K010 | SARS-CoV-2 Nucleocapsid Protein Titer Assay Kit |
| S protein | TAS-K011 | SARS-CoV-2 Spike Protein Titer Assay Kit | |
| Inhibitor screening kit | S protein RBD | EP-105 | SARS-CoV-2 Inhibitor Screening Kit |
| Antigen pre-coated plate | S1 protein | SP-12 | SARS-CoV-2 Spike S1 protein-Coated Plates, Clear, 96-Well |
| S protein RBD | RP-13 | SARS-CoV-2 Spike protein RBD-Coated Plates, Clear, 96-Well |
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