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SARS-CoV-2 Proteins and Inhibitor Screening Kit

SARS-CoV-2 Proteins and Inhibitor Screening Kit
Background

Six strains of coronaviruses (CoVs) that are able to infect humans have been identified until last year. HCoV-OC43, HCoV-229E, HCoV-NL63, and HCoVHKU1 are not highly pathogenic and only cause mild respiratory diseases. SARS-CoV and MERS-CoV have caused two large-scale pandemics. Before efficient antiviral drugs or vaccines are developed for SARS-CoV or MERS-CoV, another outbreak of pneumonia caused by a new coronavirus (SARS-CoV-2) has emerged in Wuhan since December 2019.

Coronaviruses are a group of enveloped positive-stranded RNA viruses that consist of four structural proteins including spike (S) glycoprotein, envelope (E) protein, membrane (M) protein, and nucleocapsid (N) protein. Spike glycoprotein is the most important surface protein of coronavirus, which can mediate the entrance to human respiratory epithelial cells by interacting with cell surface receptor angiotensin-converting enzyme 2 (ACE2).

Trimer Structure S Protein Verified by SEC-MALS

An article published in Science determined a 3.5-angstrom-resolution cryo-electron microscopy structure of the SARS-CoV-2 S trimer in the prefusion conformation. To facilitate medical countermeasure development, ACROBiosystems has developed the  S protein with a trimer structure. The molecular weight and polymer form of the protein was verified by SEC-MALS technology. We also offer high quality SARS-CoV-2 S1, S RBD, N, human ACE2 and other proteins. The bioactivity was verified by SPR and BLI. Protocols are offered for free.

Inhibitor Screening Kit

There is no effective treatment for COVID-19 in the market. It is urgent to develop the SARS-CoV-2 inhibitors, for example vaccines, therapeutic antibodies and small molecular compounds against COVID-19. ACRO has developed the SARS-CoV-2 inhibitor screening kit based on the biotinylated ACE2 protein and SARS-CoV-2 S protein RBD. This inhibitor screening ELISA pair is designed to facilitate the identification and characterization of SARS-CoV-2 inhibitors.

Data

Purity of SARS-CoV-2 (COVID-19)  S protein is higher than 85%, and the molecular weight is 640-680 kDa as verified by SEC-MALS.

Fig.1 The purity of SARS-CoV-2  S protein (R683A, R685A), His Tag, active trimer (Cat. No. SPN-C52H8) was more than 85% and the molecular weight is around 640-680 kDa as verified by SEC-MALS.

The affinity constant of human ACE2 protein binding to SARS-CoV-2 (COVID-19) S protein  trimer protein was 35.3 nM as verified by SPR.

Fig.2 Human ACE2, Fc Tag (Cat. No. AC2-H5257) Captured on CM5 chip via anti-human IgG Fc antibodies surface can bind SARS-CoV-2  S protein (R683A, R685A), His Tag, active trimer (Cat. No. SPN-C52H8) with an affinity constant of 35.3 nM as determined in a SPR assay (Biacore 8K).

The linear range of biotinylated human ACE2 protein binding to SARS-CoV-2 (COVID-19) S protein  trimer protein was 4-63 ng/mL as verified by ELISA.

Fig.3 Immobilized SARS-CoV-2  S protein (R683A, R685A), His Tag, active trimer (Cat. No. SPN-C52H8) at 2 μg/mL (100 μL/well) can bind Biotinylated Human ACE2, His,Avitag (Cat. No. AC2-H82E6) with a linear range of 4-63 ng/mL.

Product List

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Kits  For research use only.
MoleculeCat. No.SpeciesProduct DescriptionOrder/Preorder
S protein RBDEP-105SARS-CoV-2SARS-CoV-2 Inhibitor Screening Kit

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S1 proteinMBS-K001SARS-CoV-2SARS-CoV-2 Spike S1 protein-coupled magnetic beads

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S protein RBDMBS-K002SARS-CoV-2SARS-CoV-2 Spike protein RBD-coupled magnetic beads

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TAS-K003SARS-CoV-2Anti-SARS-CoV-2 neutralizing antibody titer serologic assay kit

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S1 proteinSP-12SARS-CoV-2SARS-CoV2 Spike S1 protein-Coated Plates, Clear, 96-Well

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S protein RBDRP-13SARS-CoV-2SARS-CoV2 Spike protein RBD-Coated Plates, Clear, 96-Well

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S1 proteinTAS-K001SARS-CoV-2SARS-CoV-2 antibody titer assay kit (Spike protein S1)

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S protein RBDTAS-K002SARS-CoV-2SARS-CoV-2 antibody titer assay kit (Spike protein RBD)

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Antibody
MoleculeCat. No.SpeciesProduct DescriptionOrder/Preorder
S protein RBDSAD-S35HumanAnti-SARS-CoV-2 neutralizing antibody (Human IgG1)

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Proteins
MoleculeCat. No.SpeciesHostProduct DescriptionOrder/Preorder
ACE2AC2-H82E6HumanHEK293Biotinylated Human ACE2 / ACEH Protein, His,Avitag™ (MALS verified)

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AC2-H82F9HumanHEK293Biotinylated Human ACE2 / ACEH Protein, Fc,Avitag™

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AC2-H5257HumanHEK293Human ACE2 / ACEH Protein, Fc Tag (MALS verified)

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AC2-C52H7CynomolgusHEK293Cynomolgus ACE2 / ACEH Protein, His Tag

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AC2-H52H8HumanHEK293Human ACE2 / ACEH Protein, His Tag (MALS verified)

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AC2-R5246RatHEK293Rat ACE2 / ACEH Protein, His Tag (MALS verified)

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AC2-M5248MouseHEK293Mouse ACE2 / ACEH Protein, His Tag (MALS verified)

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AC2-P5248Paguma larvataHEK293Paguma larvata ACE2 / ACEH Protein, His Tag

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S proteinSPN-C52H4SARS-CoV-2HEK293SARS-CoV-2 (COVID-19)  S protein (R683A, R685A), His Tag

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SPN-C52H8SARS-CoV-2HEK293SARS-CoV-2 (COVID-19)  S protein (R683A, R685A) , His Tag, active trimer (MALS verified)

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SPN-S52H5SARSHEK293SARS S protein (R667A), His Tag

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S1 proteinS1N-C52H3SARS-CoV-2HEK293SARS-CoV-2 (COVID-19) S1 protein, His Tag

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S1N-C82E8SARS-CoV-2HEK293Biotinylated SARS-CoV-2 (COVID-19) S1 protein, His,Avitag™ (MALS verified)

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S1N-S52H5SARSHEK293SARS S1 protein, His Tag (MALS verified)

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S1N-C52H4SARS-CoV-2HEK293SARS-CoV-2 (COVID-19) S1 protein, His Tag (MALS verified)

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S1N-C5255SARS-CoV-2HEK293SARS-CoV-2 (COVID-19) S1 protein, Fc Tag

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S1N-C5257SARS-CoV-2HEK293SARS-CoV-2 (COVID-19) S1 protein, Mouse IgG2a Fc Tag

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S protein RBDSPD-C82E9SARS-CoV-2HEK293Biotinylated SARS-CoV-2 (COVID-19) S protein RBD, His,Avitag™ (MALS verified)

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SPD-C5255SARS-CoV-2HEK293SARS-CoV-2 (COVID-19) S protein RBD, Fc Tag (MALS verified)

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SPD-S52H6SARSHEK293SARS S protein RBD, His Tag (MALS verified)

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SPD-C52H3SARS-CoV-2HEK293SARS-CoV-2 (COVID-19) S protein RBD, His Tag (MALS verified)

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SPD-C5259SARS-CoV-2HEK293SARS-CoV-2 (COVID-19) S protein RBD, Mouse IgG2a Fc Tag

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SPD-S52H4SARS-CoV-2HEK293SARS-CoV-2 (COVID-19) S protein RBD (V367F), His Tag

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SPD-S52H5SARS-CoV-2HEK293SARS-CoV-2 (COVID-19) S protein RBD (N354D), His Tag

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SPD-S52H7SARS-CoV-2HEK293SARS-CoV-2 (COVID-19) S protein RBD (W436R), His Tag

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SPD-S52H8SARS-CoV-2HEK293SARS-CoV-2 (COVID-19) S protein RBD (R408I), His Tag

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SPD-S52H3SARS-CoV-2HEK293SARS-CoV-2 (COVID-19) S protein RBD (N354D, D364Y), His Tag

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Nucleocapsid proteinNUN-C51H9SARS-CoV-2E.coliSARS-CoV-2 (COVID-19) Nucleocapsid protein, His Tag

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NUN-C5227SARS-CoV-2HEK293SARS-CoV-2 (COVID-19) Nucleocapsid protein, His Tag

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NUN-C81Q6SARS-CoV-2E.coliBiotinylated SARS-CoV-2 (COVID-19) Nucleocapsid protein, His,Avitag™

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Envelope proteinENN-C5128SARS-CoV-2E.coliSARS-CoV-2 (COVID-19) Envelope protein, His Tag

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Papain-like ProteasePAE-C5148SARS-CoV-2E.coliSARS-CoV-2 (COVID-19) Papain-like Protease Protein, His Tag

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NSP1NS1-C51H7SARS-CoV-2E.coliSARS-CoV-2 (COVID-19) NSP1 Protein, His Tag

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NSP7NS7-C51H6SARS-CoV-2E.coliSARS-CoV-2 (COVID-19) NSP7 Protein, His Tag

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NSP8NS8-C5149SARS-CoV-2E.coliSARS-CoV-2 (COVID-19) NSP8 Protein, His Tag

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NSP7 & NSP8NS8-C5125SARS-CoV-2E.coliSARS-CoV-2 (COVID-19) NSP7&NSP8 Protein, His Tag

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S1 proteinS1N-C5256SARS-CoV-2HEK293SARS-CoV-2 (COVID-19) S1 protein (D614G), His Tag

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Expected launch date: Jun. 4

SIN-V52H3HCoV-NL63HEK293HCoV-NL63 S1 protein, His Tag

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SIN-V52H4HCoV-229EHEK293HCoV-229E S1 protein, His Tag

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SIN-V52H5HCoV-OC43HEK293HCoV-OC43 S1 protein, His Tag

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SIN-V52H6HCoV-HKU1(isolate N5)HEK293HCoV-HKU1(isolate N5) S1 protein, His Tag

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S1 protein CTDS1D-C52H3SARS-CoV-2HEK293SARS-CoV-2 (COVID-19) S1 protein CTD, His Tag

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S1 protein NTDS1D-C52H6SARS-CoV-2HEK293SARS-CoV-2 (COVID-19) S1 protein NTD, His Tag

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S protein RBDSPD-C52H4SARS-CoV-2HEK293SARS-CoV-2 (COVID-19) S protein RBD (G476S), His Tag

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SPD-C52H5SARS-CoV-2HEK293SARS-CoV-2 (COVID-19) S protein RBD (V483A), His Tag

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S2 proteinS2N-C5253SARS-CoV-2HEK293SARS-CoV-2 (COVID-19) S2 protein, Fc Tag

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Expected launch date: Jun. 3

S2N-C5254SARS-CoV-2HEK293SARS-CoV-2 (COVID-19) S2 protein, Mouse IgG2a Fc Tag

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Expected launch date: Jun.1

Envelope proteinENN-C51P3SARS-CoV-2E.coliSARS-CoV-2 (COVID-19) Envelope protein, His,BBP Tag

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Expected launch date: Jun. 11

NSP15NS5-C5145SARS-CoV-2E.coliSARS-CoV-2 (COVID-19) NSP15 Protein, His Tag

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Expected launch date: May. 31

NSP16 & NSP10NS0-C51W3SARS-CoV-2E.coliSARS-CoV-2 (COVID-19) NSP16&NSP10 Heterodimer Protein, His Tag&Twin Strep Tag

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Expected launch date: May. 31

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