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Items | Size (2mg) | Size(5mg X 2) |
Particle size | 2 μm | 2 μm |
Physical appearance | Powder mixture | Powder mixture |
Amount of Coupled Protein | ≈939 pmol (46 μg) Nucleocapsid protein/mg beads | ≈939 pmol (46 μg) Nucleocapsid protein/mg beads |
Binding Capacity | >130 pmol (20 μg) Anti-Nucleocapsid antibody/mg beads | >130 pmol (20 μg) Anti-Nucleocapsid antibody/mg beads |
Formulation | PBS, pH7.4, with 10% Trehalose | PBS, pH7.4, with 10% Trehalose |
Reconstitution | 2 mL sterile deionized water (1 mg beads/mL) | 5 mL sterile deionized water (1 mg beads/mL) |
See Certificate of Analysis (CoA) for detailed instruction.
The magnetic beads technology makes use of the easy and efficient collection of beads in magnetic field to facilitate antibody purification in a simple workflow of “bind-wash-elute”. In contrast to common separation techniques, this method does not require columns or centrifugation, and is therefore ideal in high-throughput applications.
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Immobilized 49 μg SARS-CoV-2 Nucleocapsid protein to 1 mg Beads can bind the Anti-SARS-CoV-2 Nucleocapsid Antibody with an EC50 of 0.6712 μg/mL.
Accelerated stability test. After placing the lyophilized beads at 37°C for 7 days, binding activity between the SARS-CoV-2 Nucleocapsid Protein-coupled Magnetic Beads (Cat.No. MBS-K017) and the Anti-SARS-CoV-2 Nucleocapsid Antibody showed little deviation from the unaccelerated sample (%RSD<10%). Data were measured on day 0, 3, 7 respectively.
Freeze-thaw stability test. After different freeze-thaw cycles, binding activity between the SARS-CoV-2 Nucleocapsid Protein-coupled Magnetic Beads (Cat.No. MBS-K017) and the Anti-SARS-CoV-2 Nucleocapsid Antibody showed little deviation from the unfreeze-thaw sample (%RSD<10%). Three freeze-thaw cycles were performed.
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