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The kit is used for quantitative detection of residual E. coli DNA in biopharmaceutical production.
It is for research use only.
Figure 1. High sensitivity and broad dynamic range using the E. coli residual DNA quantitative Kit. (A) Typical analysis results obtained with Standard 1 (300 pg/μL) to 6 (3 fg/μL). (B) The standard curve of the 10-fold dilution series. PCR efficiency should be 90-110%.
Figure 2. Assay specificity. Standard curves generated using 10-fold serial dilution of E. coli genomic DNA (included in the kit).
Figure 3. Consistent quantitation across a broad range of fragment sizes. Standard curves were generated using a 10-fold serial dilution of gDNA and fragmented DNA. Fragmented DNA was generated by sonicating total E. coli genomic DNA (8min, 13min, 20min). Fragmentation of the DNA was confirmed by agarose gel analysis.
Figure 4. The amplification efficiency of standard curve (ACRO and Competitor T) is close to 100%, while the amplification efficiency of standard curve (Competitor H) is less than 90%.
Figure 5. Validation of the recovery in four different E. coli DNA samples (gDNA 180 pg, gDNA 1.8 pg, Fragmented DNA 180 pg, Fragmented DNA 1.8 pg). Results show that the recovery using the ACRO products are between 90 and 100%.
| ID | Components | Size |
| OPA-O002-01 | 2×qPCR Master Mix | 1.0 mL×2 |
| OPA-O002-02 | E. coli Primer & Probe Mix | 700 μL |
| OPA-O002-03 | E. coli DNA Control（3ng/μL） | 100 μL |
| OPA-O002-04 | Dilution Buffer | 1.5 mL×3 |
| OPA-O002-05 | DNase/RNase-free Water | 1.0 mL |
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