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| ID | Components | Size |
| RIPO-BWM001K-C01 | Basal medium A | 22.5ml |
| RIPO-BWM001K-C02 | Basal medium B | 22.5ml |
| RIPO-BWM001K-C03 | Basal medium MM | 90ml |
| ID | Components | Size |
| RIPO-BWM001K-1-C01 | Supplement A-1 | 2ml |
| RIPO-BWM001K-1-C02 | Supplement A-2 | 0.5ml |
| RIPO-BWM001K-1-C03 | Supplement B-1 | 2ml |
| RIPO-BWM001K-1-C04 | Supplement B-2 | 0.5ml |
| RIPO-BWM001K-1-C05 | Supplement MM-1 | 8ml |
| RIPO-BWM001K-1-C06 | Supplement MM-2 | 2ml |
The unopened Box A is stable for 12 months from the date of manufacture if stored under 2-8°C.
The unopened Box B is stable for 12 months from the date of manufacture if stored under -20°C.
The opened kit should be stored according to components table.
Protocol Diagram of cerebral organoid differentiation.
Left: Early-stage cerebral organoid show rosette-like structures (neural stem cells), which become smaller as organoids develop.
Right: Day 109 cerebral organoids show uniform morphology and show no dead core inside.
Presence of NESTIN positive cells and TUJ1 positive cells in early stage (day 36) cerebral organoid.
TUJ1: early-stage neuronal marker.
NESTIN : a cytoskeletal intermediate filament characterized in neural stem cells.
Presence of TH and MAP2 positive neurons in day 92 cerebral organoid.
TH: used as cell marker of dopaminergic neurons.
MAP2: mature neuron cell marker.
Left: Presence of GFAP positive cells at day 109 cultured cerebral organoid.
Right: Presence of OLIG2 positive cells at day 119 cultured cerebral organoid.
GFAP: marker for astrocyte.
OLIG2: marker for oligodendrocyte.
Presence of TH and IBA1 positive cell in day 147 cultured cerebral organoid.
IBA1: cell marker for microglia.
A: patch-clamp recording of excitatory neurons sectioned from cerebral organoids at day 102. Excitatory neuron showed stable response to step injection currents.
B: Recording of cerebral organoid (day 60) using silicon probe. Neurons showed spontaneous activities and different waveforms.
C: MEA recording indicated spontaneous bursting activities for cerebral organoid (day 86).
Using cerebral organoids for modelling Parkinson's Disease. Cerebral organoids are treated with Human Alpha-Synuclein Pre-formed Fibrils Protein (Ca. No. ALN-H51H4). Dopaminergic neurons and MAP2 positive neurons are damaged with both 0.1μM and 1μM Alpha-Synuclein Pre-formed Fibrils.
AAVs selection using cerebral organoids: Different capsid of AAVs were incubated with cerebral organoids. As the results, different affinities of each AAV to the cerebral organoid were observed.
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