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In our efforts to provide the right blend of innovation and value to our clients, ACROBiosystems has NEW Lab Services! Our scientists have implemented and mastered numerous assay platforms over the years. Our recent introduction of the INNOVATION LAB offers TCR Screening Technology and the MSD SQ120 into our assay development portfolio.

  • MSD – ECL Multiplexing Services

MSD-ECL Platform: Broad Range Multiplex Detection via Electrochemiluminescence

ACROBiosystems offers an array of MSD-based immunoassays with a proven increase in both dynamic range and sensitivity, made possible through electro chemiluminescent labeling. ACROBiosystems utilizes our expertise to accurately access clients’ needs and to guide in the design of assays more sensitive than traditional ELISA.

i. How the Meso Scale Discovery Platform (MSD-ECL) Works?

Using electrochemiluminescently-labeled detection antibodies, the MSD-ECL platform detects excitation near electrodes located in the bottom of each microplate. As electricity is applied to the plate electrodes, the electrochemiluminescent labels are stimulated to emit light. The intensity of those emissions is then converted to a quantitative measurement of the target analytes in cell supernatant, serum, plasma or whole blood.

ii. Ideal MSD Applications: For Multiplexing and Complex Matrices

The MSD-ECL platform offers great flexibility with stable, non-radioactive, and easily conjugated labels. Because of these factors, this technology can be applied to all types of traditional ELISAs, such as direct, indirect, sandwich or competitive assays at an increased sensitivity level that can detect samples with concentrations as low as 0.1pg/mL.

We have also found MSD assays are optimal for complex matrices as they typically yield low interference from components like serum or plasma. MSD is particularly advantageous in that its bridging formats are not specific for species or antibody subclass.

Examples of samples:
  1. Tissue culture: Quantification of protein in tissue culture supernatant.

  2. Serum/plasma: Serum/plasma kits provide assay-specific components for the quantitative determination of biomarkers in serum and plasma samples.

  3. Whole cell lysate: Detection of biomarkers in whole cell lysates.

  4. Other: Common high-throughput screening and assay development.

Meso Scale Discovery (MSD-ECL) can be applied in our work with biomarker screening, immunogenicity, pharmacokineƟcs (PK), pharmacodynamics (PD) and CAR-T cytotoxicity.

  • Biomarker
  • Immunogenicity
  • Pharmaceutical
  • 微信图片_20181218090140.png

    CAR-T cytotoxicity
iii. Key MSD-ECL Benefits: High Sensitivity and Broad Dynamic Range
Top Benefits of MSD Assays:
  • Ultra-Sensitive

  • Small Sample Volumes

  • Multiplexing

  • Broad Dynamic Range

  • Low Background

  • High Throughput

MSD’s proprietary multi-array technology can provide significantly increased sensitivity and dynamic range over standard ELISA with the added benefit of multiplexing. Fast, yet continuous, non-destructive reads produce consistent assay data with low sample volume requirements and minimal reagent consumption. An added benefit of the electrochemiluminescence (ECL) detection technology is that it reduces assay background by separating the excitation mechanism from the signal.

With a broad range of quantitation, assays conducted using MSD technology are cost-effective and exhibit a reduced need for repeats stemming from readings above the ULOQ or below the LLOQ. This means more valid data points from sample sets in a single run than with other similar platforms.

The combination of rapid read times and the ability to perform multiple, simultaneous tests on a single sample increases productivity, conserves sample, and delivers results quickly.

iv. Case Study: Analysis of Proinflammatory Cytokines in Mouse Serum

Samples: Mouse Serum


10 Mouse Cytokines: IFN-γ, IL-10, IL-12p70, IL-1β, IL-2, IL-4, IL-5, IL-6, KC/GRO, TNF-α

4 Human Cytokines: FasL, Granzyme B, IFN-γ, IL-1β

  1. Up to 10 analytes can be processed in the same panel,which is very efficient and cost effective

  2. Large linear range can be 3 to 4+ log

  3. MSD exhibits excellent sensitivity,precision,minimal matrix effect and free drug interaction

V. Samples and Turnaround
  1. Sample requirements: Serum,plasma and cell culture supernatants are all compatible with the platform.
    Sample volume depends on desired analytes amout.(25ul sample per panel)

  2. Turnaround time: MSD operation time mainly depends on sample size and the number of analytes.
    Conservatively,5 to 10 business days. Please contact us to get an estimate.

  3. What is provided to the clients: Raw data file and data report.

Please complete this questionnaire to receive additional information related to your service and/or product needs. An ACROBiosystems scientist will respond to your request within 24 hours of submission.
Biomarker Screening
Sample Analysis
Assay Development
PK Study
ELISA Conversion
For additional information about ACROBiosystems Innovation Lab’s platforms or services, please call us at: +1 302-644-5472 or email at: services@acrobiosystems.com.

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