>Fc Receptor Proteins
The efficacy of a therapeutic antibody not only depends on the Fab fragment and its binding activity to the target antigen, but also depends on the Fc fragment and its interaction with key Fc receptors. The binding affinity of the Fc fragment towards FcRn (FCGRT&B2M) would predict the antibody’s half life, while that between the Fc fragment and FCGRIIIA (CD16a) would influence the antibody’s ability to elicit ADCC (antibody dependent cell mediated cytotoxicity). Hence, screening for desired binding affinity to these Fc receptors is an essential component in the development of a therapeutic mAb (monoclonal antibody).
ACROBiosystems offers a comprehensive collection of recombinant Fc receptor proteins, including their common variants, to help expedite your mAb development.
|Molecule||Catalog No.||Species||Product Name||Structure||Purity||Features|
|FcRn / FCGRT|
|FCM-H5286||FcRn / FCGRT & B2M Heterodimer Protein|
|FCM-H8286||Biotinylated FcRn / FCGRT & B2M, ultra sensitivity (primary amine labeling)|
|FCM-H82W4||Biotinylated FcRn / FCGRT & B2M, Avi Tag (Avitag™)|
|FCM-M52W2||FcRn / FCGRT & B2M Heterodimer Protein|
|FCM-M82W6||Biotinylated FcRn / FCGRT & B2M, Avi Tag (Avitag™)|
|FCM-R5287||FcRn / FCGRT & B2M Heterodimer Protein|
|FCM-R82W7||Biotinylated FcRn / FCGRT & B2M, Avi Tag (Avitag™)|
|FCM-C5284||FcRn / FCGRT & B2M Heterodimer Protein|
|FCM-C82W5||Biotinylated FcRn / FCGRT & B2M, Avi Tag (Avitag™)|
|FCM-P5280||FcRn / FCGRT & B2M Heterodimer Protein|
|FCN-F82W3||Biotinylated Feline FcRn / FCGRT & B2M Heterodimer Protein, His Tag & Avi Tag (Avitag™)|
|Fc gamma RIIIA / CD16a||CD8-H52H4||FCGR3A / CD16a (V176) Protein|
|CDA-H5220||FCGR3A / CD16a (F176) Protein|
|CDA-H82E8||Biotinylated Fc gamma RIIIA / CD16a (F176), Avi Tag (Avitag™)|
|CDA-H82E9||Biotinylated Fc gamma RIIIA / CD16a (V176), Avi Tag (Avitag™)|
|CDA-M52H8||FCGR3A / CD16a Protein|
|Fc gamma RIIIB / CD16b||CDB-H5222||Human Fc gamma RIIIB / CD16b (NA2) Protein|
|CDB-H82E4||Biotinylated Human Fc gamma RIIIB / CD16b (NA1), His Tag & Avi Tag (Avitag™)|
|CDB-H5227||Human Fc gamma RIIIB / CD16b (NA1) Protein, His tag|
|Fc gamma RIIA / CD32a||CD1-H5223||FcGR2A / CD32a (H167) Protein|
|CDA-H5221||Fc gamma RIIA / CD32a (R167) Protein|
|CDA-H82E6||Biotinylated Fc gamma RIIA / CD32a (H167), Avi Tag (Avitag™)|
|CDA-H82E7||Biotinylated Fc gamma RIIA / CD32a (R167), Avi Tag (Avitag™)|
|Fc gamma RIIB / CD32b||CDB-H5228||Fc gamma RIIB / CD32b Protein|
|Fc gamma RI / CD64||FCA-H52H2||Fc gamma RI / CD64 Protein|
|CD4-M5227||Fc gamma RI / CD64 Protein|
|FCA-H82E8||Biotinylated Human Fc gamma RI / CD64, His Tag, Avi Tag (Avitag™)|
|CD23||CD3-H82Q5||Biotinylated Human CD23 / Fc epsilon RII Protein, His Tag & Avi Tag (Avitag™)|
|Fc gamma RIII / CD16||FC6-C82E0||Biotinylated Cynomolgus FCGR3 / CD16 Protein, His Tag & Avi Tag (Avitag™)|
|FC6-C52H9||Cynomolgus FCGR3 / CD16 Protein, His Tag|
ACROBiosystems Fc receptor proteins are exclusively produced in HEK293 cells. The proteins retain their authentic post-translational processing, which often translates into high bioactivity and stability.
The consistency in purity and bioactivity is essential in the successful production of biotinylated proteins. All our proteins are evaluated by a variety of methods throughout the production process to ensure all relevant requirements are met.
Purity greater than 95% by SDS-PAGE and SEC-HPLC
Bioactivity validated in functional ELISA and SPR
Endotoxin less than 1EU/µg by the LAL method.
Fig. 1 The purity of human FcRn (Cat. No. FCM-H5286) was greater than 95% as determined in a HPLC analysis.
Fig. 2 Immobilized Human FcRn / FCGRT & B2M Heterodimer Protein (Cat. No. FCM-H5286) on CM5 Chip, can bind Herceptin with an affinity constant of 0.427 μM as determined in SPR assay (Biacore 8K).
The use of biotin labeling can make your assay development much easier. We offer a variety of ready-to-use biotinylated Fc receptors. These proteins are produced using our in-house developed labeling techniques, which confers high bioactivity and minimal batch-to-batch variation.
Fig. 3 Immobilized Biotinylated Human FcRn / FCGRT & B2M Heterodimer Protein (Cat.No. FCM-H82W4) on SA chip, can bind Herceptin with an affinity constant of 1.47 μM as determined in a SPR assay (Biacore T200).
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