Rat FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag (SPR verified), produced by co-expression of FCGRT and B2M, has a calculated MW of 31.8 kDa (FCGRT) and 13.1 kDa (B2M). Subunit FCGRT is fused with a polyhistidine tag at the C-terminus and subunit Beta-2 microglobulin (B2M) is fused with Strep II-tag at the C-terminus. The reducing (R) protein migrates as 43-55 kDa (FCGRT) and 14 kDa (B2M) respectively due to different glycosylation.
>95% as determined by SDS-PAGE.
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Rat FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag (SPR verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Rat FCGRT&B2M Heterodimer Protein, His Tag&Strep II Tag (SPR verified) (Cat. No. FCM-R5287) captured on NTA-Series S sensor chip can bind Herceptin® with an affinity constant of 19.9 nM as determined in a SPR assay (Biacore T200) (QC tested).
Authors: Kenniston JA, et al.
Journal: J Biol Chem 2017
Application: ELISA & SPR
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