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5e5 of Anti-CD19 CAR-293 cells were stained with 100μL of 20 ug/mL Biotinylated Human CD19 (20-291) Protein, Fc,Avitag™, premium grade (Cat. No. CD9-H82F6) and negative control protein respectively, washed and then followed with 2.5 μg/mL of Streptavidin Protein-CY3 (Cat. No. STN-NC114) and analyzed with FACS. PE signal was used to evaluate the binding activity (QC tested).
5e5 of Anti-CD19 CAR-293 cells were stained with 100 μL of 20 ug/mL Biotinylated Human CD19 (20-291) Protein, Fc, Avitag™, premium grade (Cat. No. CD9-H82F6) and negative control protein respectively, washed and then followed with 2.5 μg/mL of Streptavidin Protein-Cy5 (Cat. No. STN-NC113) and analyzed with FACS. Cy5 signal was used to evaluate the binding activity (QC tested).
Immobilized Biotinylated Canine PD-1, His&Avi tag at 1000 ng/mL (100 μL/well) on Streptavidin Coated Plates, Clear, 96-Well, Clear Frame (For Serological Testing) (Cat. No. SP-15), can bind PD-L1 with a linear range of 0.01-20ng/mL, EC50=1.562ng/ml (QC tested).
The purity of Streptavidin Protein-Acridinium ester (Cat. No. STN-NA114) is more than 90% and the molecular weight of this protein is around 55-75 kDa verified by SEC-MALS.
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