5e5 of Anti-CD19 CAR-293 cells were stained with 100 μL of 20 μg/mL Biotinylated Human CD19 (20-291) Protein, Fc,Avitag, premium grade (Cat. No. CD9-H82F6) and negative control respectively, washed and then followed with 2.5 μg/mL of Streptavidin Protein-APC, premium grade (Cat. No. STN-NA112) and analyzed with FACS. APC signal was used to evaluate the binding activity (QC tested).
5e5 of Anti-CD19 CAR-293 cells were stained with 100μL of 20 ug/mL Biotinylated Human CD19 (20-291) Protein, Fc,Avitag™, premium grade (Cat. No. CD9-H82F6) and negative control protein respectively, washed and then followed with 2.5 μg/mL of Streptavidin Protein-CY3 (Cat. No. STN-NC114) and analyzed with FACS. PE signal was used to evaluate the binding activity (QC tested).
Immobilized Biotinylated Human Dkk-1, Fc,Avitag (Cat. No. DK1-H82F5) at 5 μg/mL (100 μL/well) on Streptavidin (A24C) Protein, His Tag (Cat. No. STN-N51H3) precoated (0.5 μg/well) plate can bind Human LRP-5, Mouse IgG2a Fc Tag (Cat. No. LR5-H5254) with a linear range of 0.5-16 ng/mL (QC tested).
The purity of Recombinant Streptavidin Protein, premium grade (Cat. No. STN-N5114) is more than 95% and the molecular weight of this protein is around 45-60 kDa verified by SEC-MALS.
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