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Your Position: Home > Fc Receptor Proteins > Fc gamma RIIA / CD32a > CDA-H5221

Human Fc gamma RIIA / CD32a (R167) Protein, His Tag (BLI verified)

  • Synonym
  • Source
    Human CD32a (R167), His Tag (BLI verified) (CDA-H5221) is expressed from human 293 cells (HEK293). It contains AA Ala 36 - Ile 218 (Accession # P12318-1 (R167)).
    Predicted N-terminus: Ala 36
  • Molecular Characterization
    Online(Ala 36 - Ile 218) P12318-1 (R167)

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 21.6 kDa. The protein migrates as 25-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;

    2. -70°C for 3 months under sterile conditions after reconstitution.

Human CD32a (R167), His Tag (BLI verified) (Cat. No. CDA-H5221) SDS-PAGE gel

Human CD32a (R167), His Tag (BLI verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.


Immobilized Human CD32a (R167), His Tag (BLI verified) (Cat. No. CDA-H5221) on CM5 Chip via anti-His antibody, can bind  Rituximab with an affinity constant of 3.12 μM as determined in a SPR assay (Biacore T200) (Routinely tested).


Loaded Rituximab on Protein A Biosensor, can bind Human CD32a (R167), His Tag (BLI verified) (Cat. No. CDA-H5221) with an affinity constant of 1.5 μM as determined in BLI assay (ForteBio Octet Red96e) (QC tested).


Loaded Human CD32a (R167), His Tag (BLI verified) (Cat. No. CDA-H5221) on HIS1K Biosensor, can bind Rituximab with an affinity constant of 1.4 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

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