|Items||Size （2mg）||Size（5mg X 2）|
|Particle size||2 μm||2 μm|
|Physical appearance||Powder mixture||Powder mixture|
|Amount of Coupled Protein||>287 pmol (40μg) SARS-CoV-2 (Delta) Spike Trimer/mg beads||>287 pmol (40μg) SARS-CoV-2 (Delta) Spike Trimer/mg beads|
|Binding Capacity||>108 pmol (15μg) ACE2/mg beads||>108 pmol (15μg) ACE2/mg beads|
|Formulation||PBS, pH7.4, with 10% Trehalose||PBS, pH7.4, with 10% Trehalose|
|Reconstitution||2 mL ultrapure water (1 mg beads/mL)||5 mL ultrapure water (1 mg beads/mL)|
Upon receipt, please store the beads at -20℃ for 1 year in lyophilized state.
Please avoid more than 3 freeze-thaw cycles. Immediate use after reconstitution is highly recommended.
1. Resuspend the lyophilized beads by adding the buffer of choice.
2. Add analyte to the suspension, mix and incubate to enable specific binding of the beads and the target protein.
3. Magnetize beads, remove supernatant, and wash unbound protein fractions to capture target protein-bound beads.
4. Wash, magnetize the beads and collect purified target protein for use in downstream applications.
Immobilized 46.8 μg SARS-CoV-2 (Delta) Spike Trimer/1mg Beads can bind human ACE2 (Cat. No. AC2-H5257) with an EC50 of 1.260 μg/mL (QC tested).
Accelerated stability test. After placing the lyophilized beads at 37℃ for 7 days, binding activity between the SARS-CoV-2 (Delta) Spike Trimer-coupled Magnetic Beads (Cat.No. MBS-K038) and Human ACE2 proteins showed little deviation from the unaccelerated sample (%RSD<15%). Data were measured on day 0, 3, 7 respectively.
Freeze-thaw stability test. After different freeze-thaw cycles, binding activity between the SARS-CoV-2 (Delta) Spike Trimer-coupled Magnetic Beads (Cat.No. MBS-K038) and Human ACE2 proteins showed little deviation from the unfreeze-thaw sample (%RSD<10%). Three freeze-thaw cycles were performed.
Price(USD) : $1300.00
Price(USD) : $3990.00
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