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Your Position: Home > Leukocyte Surface Antigen CD47

Leukocyte Surface Antigen CD47

CD47,or Integrin-associated protein (IAP), is first discovered in a study aimed  at studying integrin αvβ3. It turned out to be associated in cis with integrin αvβ3 on the cell membrane. Followup studies has established CD47 as the quintessential "marker of self" expressed in all cell types. It's a key regulator of tissue homeostasis, and is also involved in a variety of diseases ranging from atherosclerosis to cancer. CD47 protects the host cells by suppressing phagocytosis. It's well established that cancer cell-expressed CD47 interacts with the NK cell-expressed SIRP family proteins, SIRP alpha and SIRP gamma. By so doing, it protects the cancer cell from being eliminated by the innate immune response.

In addition, CD47 is also essential for TSP-1 mediated vascular NO signaling.  It regulates CD36 and VEGFR2 to inhibit angiogenesis. Long term dysregulation  of NO signaling is a hallmark for several types of cardiovascular diseases,  which makes CD47 a promissing target for atherosclerosis. Indeed, Anti-CD47 mAb  has been demonstrated to reduce the atherosclerotic burden in several in vivo  models.

CD47 proteins

Given its significant role in pathogenesis of cancer and cardiovascular diseases, there is no surprise that CD47 signaling has been heavily investigated for new targeted therapeutics. ACROBiosystems provides a unique set of CD47-relevant products, including mutilple avi tag pre-biotinylated proteins, for rapid high throughput screening. The following pairs of products can be easily adapted to ELISA, Alphalisa, and MSD platform to screen neutralizing compound.

Product Validation Data

FACS analysis showed that human CD47 can bind to SIRP-α, and the binding signal was inhibited by neutralizing SIRP-α antibody.
FACS analysis of human CD47 protein, Fc tag binding to ACHN cell surface SIRP-α

Fig.1 FACS assay shows that Human CD47, Fc Tag (HPLC-verified) (Cat. No. CD7-H5256) can bind to ACHN cell expressing SIRP-a. The concentration of CD47 used is 10 μg/mL.

 

Competitive experiment of neutralizing human CD47, Fc tag
 

Fig.2 FACS analysis shows that the binding of Human CD47 to ACHN expressing SIRP-a was inhibited by increasing concentration of neutralizing SIRP-a antibody with the IC50 15.02 μg/mL. The concentration of Human CD47 used is 10 μg/mL.

BLI verified that the affinity constant of anti-human CD47 mAb to human CD47 protein was 6.18 nM.

Fig.3 Loaded Anti-Human CD47 mAb (Human IgG4) on AHC Biosensor, can bind Human CD47, His Tag (Cat. No. CD7-H5227) with an affinity constant of 6.18 nM as determined in BLI assay (ForteBio Octet Red96e).

ELISA verified that the linear range of SIRP-α to human CD47 protein was 8-250ng/mL. The binding signal was inhibited by anti-human neutralizing antibody with the IC50 is 124.5 ng/mL.

Fig.4 Immobilized Human SIRP alpha, His Tag (HPLC-verified) (Cat. No. SIA-H5225) at 2 μg/mL (100 μL/well) can bind Biotinylated Human CD47, Fc,Avitag (Cat. No. CD7-H82F6) with a linear range of 8-250 ng/mL.

 

Fig.5 Serial dilutions of Anti-Human CD47 Neutralizing Antibody were added into Human SIRP alpha, His Tag (HPLC-verified) (Cat. No. SIA-H5225): Biotinylated Human CD47, Fc,Avitag (Cat. No. CD7-H82F6) binding reactions. The half maximal inhibitory concentration (IC50) is 124.5 ng/mL.

Product List

Inhibitor Screening Kit
MoleculeCat. No.Product DescriptionApplication
CD47EP-102CD47: SIRP alpha [Biotinylated] Inhibitor Screening ELISA Assay PairThis pair is useful for screening for inhibitors of human CD47 binding to human SIRP alpha.
CD47 Product List
MoleculeCat. No.Product DescriptionStructurePurityFeatures
CD47CD7-H5227Human CD47 Protein, His TagCD7-H5227-STC1

CD7-H82E9Biotinylated Human CD47 Protein, His,Avitag™CD7-H82E9-STC1

CD7-H52A5Human CD47 Protein, Mouse IgG2a Fc Tag, low endotoxinCD7-H52A5-STC1

CD7-HA2E9Unconjugated Human CD47 Protein, His,Avitag™CD7-HA2E9-STC1

CD7-H522aHuman CD47 Protein, His Tag, low endotoxinCD7-H522a-STC1

CD7-H5256Human CD47 Protein, Fc Tag (HPLC-verified)CD7-H5256-STC1

CD7-H5251Human CD47 Protein, Llama IgG2b Fc Tag, low endotoxinCD7-H5251-STC1
 
CD7-M82E4Biotinylated Mouse CD47 Protein, His,Avitag™CD7-M82E4-STC1

CD7-H82F6Biotinylated Human CD47 Protein, Fc,Avitag™CD7-H82F6-STC1

CD7-H82F8Biotinylated Human CD47 Protein, Fc (L234A, L235A, P329G),Avitag™CD7-H82F8-STC1

CD7-M522bMouse CD47 Protein, His TagCD7-M522b-STC1

CD7-M5251Mouse CD47 Protein, Fc TagCD7-M5251-STC1

CD7-M525aMouse CD47 Protein, Mouse IgG2a Fc Tag, low endotoxinCD7-M525a-STC1

CD7-C52H1Cynomolgus / Rhesus macaque CD47 Protein, His TagCD7-C52H1-STC1

CD7-C5252Cynomolgus / Rhesus macaque CD47 Protein, Fc TagCD7-C5252-STC1

CD7-R5256Rat CD47 Protein, Fc TagCD7-R5256-STC1

CD7-R5257Rabbit CD47 Protein, Fc TagCD7-R5257-STC1
 
CD7-C52H5Canine CD47 Protein, His TagCD7-C52H5-STC1

Relevant Products
MoleculeCat. No.Product DescriptionStructurePurityFeatures
SIRP alphaSIA-M5258Mouse SIRP alpha / CD172a Protein, Fc TagSIA-M5258-STC1

SIA-H52A8Human SIRP alpha / CD172a Protein, Mouse IgG1 Fc Tag (HPLC-verified)SIA-H52A8-STC1

SIA-C52H7Cynomolgus SIRP alpha / CD172a Protein, His TagSIA-C52H7-STC1

SIA-HP252PE-Labeled Human SIRP alpha / CD172a Protein, Fc Tag (recommended for neutralizing assay)SIA-HP252-STC1

SIA-H5225Human SIRP alpha / CD172a Protein, His Tag (HPLC-verified)SIA-H5225-STC1

SIA-H5251Human SIRP alpha / CD172a Protein, Fc Tag (HPLC-verified)SIA-H5251-STC1

SIA-H82E0Biotinylated Human SIRP alpha / CD172a Protein, His,Avitag™ (HPLC-verified)SIA-H82E0-STC1

CDA-H82F2Biotinylated Human SIRP alpha / CD172a Protein, Fc,Avitag™CDA-H82F2-STC1

SIA-M52H4Mouse SIRP alpha / CD172a Protein, His TagSIA-M52H4-STC1

SIA-M5252Mouse SIRP alpha / CD172a Protein, Mouse IgG2a Fc Tag, low endotoxinSIA-M5252-STC1

SIA-C5254Cynomolgus SIRP alpha / CD172a Protein, Fc TagSIA-C5254-STC1

SIRP betaSIA-H5257Human SIRP beta / CD172b Protein, Fc TagSIA-H5257-STC1
 
SIRP gammaSIG-H5253Human SIRP gamma / CD172g Protein, Fc TagSIG-H5253-STC1

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References

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