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FITC-labeled and PE-labeled Proteins for biologic drug development

FITC-labeled and PE-labeled

Fluorescent-labeled proteins can be powerful tools for the detection of CAR expression and antibody screening through flow cytometry. However, the development of high-quality fluorescent-labeled proteins presents a major challenge and there are few products commercially available so far.

Positioned as a reliable partner to provide antigens for biologic drug discovery and development, ACROBiosystems has developed an exclusive collection of FITC-labeled and PE-labeled proteins by using our in-house developed chemical labeling technique. These fluorescent-labeled proteins are uniquely suitable for flow cytometry-based assays, such as detection of anti-CD19 CAR expression and cell-based ligand binding assays.

Features and benefits

  1. Bioactivity validated by flow cytometry and protocol offered

  2. Identical binding capacity before and after conjugation

  3. High batch-to-batch consistency

Case Studies

Evaluation of CAR expression by flow cytometry

FACS Analysis of Anti-CD19 CAR Expression

293 cells were transfected with anti-CD19-scFv and RFP tag. 2e5 of the cells were stained with B. FITC-Labeled Human CD19 (20-291), Fc Tag (Cat. No. CD9-HF251, 10 μg/ml) and C. FITC-labeled protein control. A. Nin-transfected 293 cells and C. FITC-labeled protein control were used as negative control. RFP was used to evaluate CAR (anti-CD19-scFv) expression and FITC was used to evaluate the binding activity of FITC-Labeled Human CD19 (20-291), Fc Tag (Cat. No. CD9-HF251) (QC tested).

Flow cytometry-based ligand binding assay

Flow Cytometry assay shows that PE-Labeled Human PD-1, Fc Tag, His Tag (recommended for neutralizing assay) (Cat. No. PD1-HP2F2) can bind to 293T cells overexpressing human PD-L1. 1μl stock solution per million cells (QC tested).

Flow cytometry-based neutralizing antibody screening assay

FACS analysis shows that the binding of PE-Labeled Human PD-1, Fc Tag, His Tag (recommended for neutralizing assay) (Cat.No.PD1-HP2F2) to 293T overexpressing PD-L1 was inhibited by increasing concentration of neutralizing antihuman PD-L1 antibody. The IC50 is 0.29 μg/ml (QC tested).

Product list

PE Labeled Protein
Molecule Cat. No. Species Product Description Host Structure
SIRP alpha SIA-HP252 Human PE-Labeled Human SIRP alpha, Fc Tag
(recommended for neutralizing assay)
HEK293
PD-1 PD1-HP2F2 Human PE-Labeled Human PD-1, Fc Tag, His Tag
(recommended for neutralizing assay)
HEK293
Mesothelin MSN-HP223 Human PE-Labeled Human Mesothelin (296-580) HEK293
FITC Labeled Protein
Molecule Cat. No. Species Product Description Host Structure
Mesothelin MSN-HF25x Human FITC-Labeled Human Mesothelin (296-580), Fc Tag HEK293
MSN-HF223 Human PE-Labeled Human Mesothelin (296-580) HEK293
MSN-HF253 Human FITC-Labeled Human Mesothelin (296-580), Fc Tag HEK293
CD30 CD0-HF2H3 Human FITC-Labeled Human CD30, His Tag HEK293
SLAMF7 SL7-HF2H7 Human FITC-Labeled Human SLAMF7, His Tag HEK293
BCMA BCA-HF2H1 Human FITC-Labeled Human BCMA, His Tag HEK293
BCA-HF254 Human FITC-Labeled Human BCMA, Fc Tag HEK293
Siglec-2 SI2-HF2H6 Human FITC-Labeled Human Siglec-2 HEK293
CD19 CD9-HF251 Human FITC-Labeled Human CD19 (20-291), Fc Tag HEK293
CD9-HF2H2 Human FITC-Labeled Human CD19 (20-291) HEK293
Glypican 3 GP3-HF258 Human FITC-Labeled Human Glypican 3, Fc Tag HEK293
GP3-HF2H1 Human FITC-Labeled Human Glypican 3, His Tag HEK293
BAFF BAF-HF268 Human FITC-Labeled Human BAFF / TNFSF13B / CD257 Protein, Fc Tag HEK293
Customized Request

We are actively developing the Fluorescent-labeled product line to fulfill our customer needs for biologic drug discovery and development. So we are highly appreciated if you would like to share your ideas and needs in this field. Please don’t hesitate to contact us if you have any suggestions or any customized request.

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