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FITC-labeled and PE-labeled Proteins for biologic drug development

FITC-labeled and PE-labeled

Fluorescent-labeled proteins can be powerful tools for the detection of CAR expression and antibody screening through flow cytometry. However, the development of high-quality fluorescent-labeled proteins presents a major challenge and there are few products commercially available so far.

Positioned as a reliable partner to provide antigens for biologic drug discovery and development, ACROBiosystems has developed an exclusive collection of FITC-labeled and PE-labeled proteins by using our in-house developed chemical labeling technique. These fluorescent-labeled proteins are uniquely suitable for flow cytometry-based assays, such as detection of anti-CD19 CAR expression and cell-based ligand binding assays.

Features and benefits

  1. Bioactivity validated by flow cytometry and protocol offered

  2. Identical binding capacity before and after conjugation

  3. High batch-to-batch consistency

Case Studies

Evaluation of CAR expression by flow cytometry

FACS Analysis of Anti-CD19 CAR Expression

293 cells were transfected with anti-CD19-scFv and RFP tag. 2e5 of the cells were stained with FITC-Labeled Human CD19 (20-291), Fc Tag (Cat. No. CD9-HF251, 10 μg/ml) and FITC-labeled protein control. Non-transfected 293 cells and FITC-labeled protein control were used as negative control. RFP was used to evaluate CAR (anti-CD19-scFv) expression and FITC was used to evaluate the binding activity of FITC-Labeled Human CD19 (20-291), Fc Tag (Cat. No. CD9-HF251) (QC tested).                        

Flow cytometry-based ligand binding assay

Flow Cytometry assay shows that PE-Labeled Human PD-1, Fc Tag, His Tag (recommended for neutralizing assay) (Cat. No. PD1-HP2F2) can bind to 293T cells overexpressing human PD-L1. 1μl stock solution per million cells (QC tested).

Flow cytometry-based neutralizing antibody screening assay

FACS analysis shows that the binding of PE-Labeled Human PD-1, Fc Tag, His Tag (recommended for neutralizing assay) (Cat.No.PD1-HP2F2) to 293T overexpressing PD-L1 was inhibited by increasing concentration of neutralizing antihuman PD-L1 antibody. The IC50 is 0.29 μg/ml (QC tested).

High batch-to-batch consistency

Binding activity of three different lots of FITC-labeled Human MSLN, Fc Tag was evaluated in the above FACS analysis against Anti-MSLN CAR-293 cells. The result shows very high batch-to-batch consistency.

Binding activity of three different lots of FITC-labeled Human MSLN, Fc Tag was evaluated in the above ELISA analysis against Anti-MSLN Antibody. The result shows very high batch-to-batch consistency.

Identical binding capacity before and after conjugation

Binding activity of the Human MSLN, Fc Tag before and after FITC labeling was evaluated in the above ELISA analysis. The result shows that FITC-Labeled and Unconjugated Human MSLN, Fc Tag have almost the same level of binding activity.

Product list

PE Labeled Protein
MoleculeCat. No.SpeciesProduct DescriptionHostStructure
CD19CD9-HP2H3HumanPE-Labeled Human CD19 (20-291) Protein, His TagHEK293
LAG-3LA3-HP2H3HumanPE-Labeled Human LAG-3 / CD223 Protein, His Tag
(recommended for neutralizing assay)
HEK293
MesothelinMSN-HP223HumanPE-Labeled Human Mesothelin (296-580)HEK293
PD-1PD1-HP2F2HumanPE-Labeled Human PD-1, Fc Tag, His Tag
(recommended for neutralizing assay)
HEK293
SIRP alphaSIA-HP252HumanPE-Labeled Human SIRP alpha, Fc Tag
(recommended for neutralizing assay)
HEK293
FITC Labeled Protein
MoleculeCat. No.SpeciesProduct DescriptionHostStructure
BAFFBAF-HF268HumanFITC-Labeled Human BAFF / TNFSF13B / CD257 Protein, Fc TagHEK293
BCMABCA-HF254HumanFITC-Labeled Human BCMA, Fc TagHEK293
BCA-HF2H1HumanFITC-Labeled Human BCMA, His TagHEK293
CD19CD9-HF251HumanFITC-Labeled Human CD19 (20-291), Fc TagHEK293
CD9-HF2H2HumanFITC-Labeled Human CD19 (20-291)HEK293
CD22SI2-HF2H6HumanFITC-Labeled Human Siglec-2HEK293
CD30CD0-HF2H3HumanFITC-Labeled Human CD30, His TagHEK293
CD4CD4-HF255HumanFITC-Labeled Human CD4 Protein, Fc TagHEK293
CD4-HF2H7HumanFITC-Labeled Human CD4 Protein, His TagHEK293
CLL-1CLA-HF247HumanFITC-Labeled Human CLEC12A / MICL / CLL-1, His TagHEK293
EGFRvIIIEGI-HF2H7HumanFITC-Labeled Human EGFRvIII Protein, His TagHEK293
ErbB3ER3-HF2H5HumanFITC-Labeled Human ErbB3 / Her3 Protein, His TagHEK293
FOLR1FO1-HF2H8HumanFITC-Labeled Human FOLR1, His TagHEK293
Glypican 3GP3-HF258HumanFITC-Labeled Human Glypican 3, Fc TagHEK293
GP3-HF2H1HumanFITC-Labeled Human Glypican 3, His TagHEK293
GUCY2CGUC-HF2H8HumanFITC-Labeled Human GUCY2C / Guanylyl cyclase C, His TagHEK293
Her2HE2-HF224HumanFITC-Labeled Human Her2 / ErbB2 Protein, His TagHEK293
IL13RA2IL2-HF2H3HumanFITC-Labeled Human IL-13 R alpha 2 Protein, His TagHEK293
MesothelinMSN-HF223HumanPE-Labeled Human Mesothelin (296-580)HEK293
MSN-HF253HumanFITC-Labeled Human Mesothelin (296-580), Fc TagHEK293
MSN-HF25xHumanFITC-Labeled Human Mesothelin (296-580), Fc TagHEK293
PSMAPSA-HF244HumanFITC-Labeled Human PSMA / FOLH1 Protein, His TagHEK293
SLAMF7SL7-HF2H7HumanFITC-Labeled Human SLAMF7, His TagHEK293
Customized Request

We are actively developing the Fluorescent-labeled product line to fulfill our customer needs for biologic drug discovery and development. So we are highly appreciated if you would like to share your ideas and needs in this field. Please don’t hesitate to contact us if you have any suggestions or any customized request.

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