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Your Position: Home > Kits > Hemagglutinin (HA) > RAS-A189

Influenza A (H1N1) Viruses Hemagglutinin (HA) Specific ELISA Kit

For research use only.

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Materials Provided

ID	Components	Size
RAS189-C01	Pre-coated Anti-HA (Influenza A (H1N1)) Antibody Microplate	1 plate
RAS189-C02	HA (Influenza A/Wisconsin/588/2019 (H1N1)) Standard	20 μg
RAS189-C03	HRP-HA (Influenza A (H1N1)) Antibody	20 μg
RAS189-C04	10×Washing Buffer	50 mL
RAS189-C05	2×Dilution Buffer	50 mL
RAS189-C06	Substrate Solution	12 mL
RAS189-C07	Stop Solution	7 mL

Background
Influenza, commonly known as 'the flu', is an infectious disease of birds and mammals caused by RNA viruses of the family Orthomyxoviridae, the influenza viruses. The virus is divided into three main types (Influenzavirus A, Influenzavirus B, and Influenzavirus C), which are distinguished by differences in two major internal proteins (hemagglutinin (HA) and neuraminidase (NA), which are the most important targets for the immune system. Hemagglutinin binds to the sialic acid-containing receptors on the surface of host cells during initial infection and at the end of an infectious cycle which makes it a great target for vaccine studies.
Application
This kit is developed for Specific quantitative detection of Influenza A (H1N1) viruses Hemagglutinin (HA) in samples.
It is for research use only.
Reconstitution
Please see Certificate of Analysis for details of reconstitution instruction and specific concentration.
Storage
1. Unopened kit should be stored at 2℃-8℃ upon receiving.
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.

Assay Principles
This assay kit employs a standard sandwich-ELISA format, providing a rapid detection of HA (Influenza A (H1N1)). The kit consists of Pre-coated Anti-HA (Influenza A (H1N1)) Antibody Microplate and HA (Influenza A/Wisconsin/588/2019 (H1N1)) Standard,HRP-HA (Influenza A (H1N1)) Antibody and buffers.
Your experiment will include 5 simple steps:
a) Bring all reagents and samples to room temperature (20℃-25℃) before use.
b) Add your sample to the plate, take the HA (Influenza A/Wisconsin/588/2019 (H1N1)) as Control sample. The samples and Control sample are diluted by Dilution Buffer.
c) Add the HRP-HA (Influenza A (H1N1)) Antibody diluted by Dilution Buffer to the plate.
d) Wash the plate and add TMB.
e) Stop the substrate reaction by adding diluted acid. Absorbance (OD) is calculated as the absorbance at 450 nm minus the absorbance at 630 nm to remove background prior to statistical analysis. The OD Value reflects the amount of protein bound.

Typical Data Please refer to DS document for the assay protocol.

For each experiment, a standard curve needs to be set for each micro-plate, and the specific OD value may vary depending on different laboratories, testers, or equipments. The following example data is for reference only.

The kit has been tested to specifically identify Influenza A [A/Wisconsin/588/2019 (H1N1)] viruses Hemagglutinin (HA), Influenza A [Victoria/4897/2022 (H1N1)] viruses Hemagglutinin (HA) and Influenza A [Wisconsin/67/2022 (H1N1)] viruses Hemagglutinin (HA) , and do not identify Influenza A Virus (A/Thailand/8/2022) & (A/Massachusetts/18/2022) HA (H3N2) , Influenza A [A/Darwin/6/2021 (H3N2)] Hemagglutinin (HA) and Influenza A [A/Darwin/9/2021 (H3N2)] Hemagglutinin (HA) Protein.