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Your Position: Home > Viral Proteins > Ebolavirus Envelope Glycoprotein 1 (GP1)(subtype Bundibugyo,strain Uganda 2007)

Recombinant Ebolavirus (subtype Bundibugyo,strain Uganda 2007) Envelope Glycoprotein 1 (GP1)

  • Synonym
    Glycoprotein GP55,Envelope glycoprotein B
  • Source
    Ebolavirus(subtype Bundibugyo,2007)GP1 (BEP-V5221) is expressed from human 293 cells (HEK293). It contains AA Ile 33 - Gln 304 (Accession # ACI28624).
    Predicted N-terminus: Ile 33
  • Molecular Characterization
    gB (HCMV)(Ile 33 - Gln 304) ACI28624

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 31.8 kDa. The protein migrates as 40-60 kDa under reducing (R) condition (SDS-PAGE).

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    No activity loss is observed after storage at:

    1. 4-8°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Ebolavirus(subtype Bundibugyo,2007)GP1 (Cat. No. BEP-V5221) SDS-PAGE gel

Ebolavirus(subtype Bundibugyo,2007)GP1 on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

  • Background
    EBOV encodes seven structural proteins: nucleoprotein (NP), polymerase cofactor (VP35), (VP40), GP, transcription activator (VP30), VP24, and RNA polymerase (L). GP protein contains 160-kDa envelope-attached glycoprotein (GP) and a 110 kDa secreted glycoprotein (sGP). GP is a class I fusion protein which assembles as trimers on viral surface and plays an important role in virus entry and attachment. Mature GP is a disulfide-linked heterodimer formed by two subunits, GP1 and GP2, which are generated from the proteolytical process of GP precursor (pre-GP) by cellular furin during virus assembly . GP1 is responsible for binding to the receptor(s) on target cells. Interacts with CD209/DC-SIGN and CLEC4M/DC-SIGNR which act as cofactors for virus entry into the host cell. GP2 acts as a class I viral fusion protein. GP1,2 mediates endothelial cell activation and decreases endothelial barrier function. sGP seems to possess an anti-inflammatory activity as it can reverse the barrier-decreasing effects of TNF alpha.
  • References
  • Please contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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Price(USD) : $260.00

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